Literature DB >> 2920023

The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain.

K Brocklehurst, M O'Driscoll, D Kowlessur, I R Phillips, W Templeton, E W Thomas, C M Topham, C W Wharton.   

Abstract

Mesh：

Substances：

Year:  1989        PMID： 2920023      PMCID： PMC1135576          DOI： 10.1042/bj2570309

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


× No keyword cloud information.
  16 in total

1.  Kinetics of papain-catalyzed hydrolysis of -N-benzoyl-L-arginine-p-nitroanilide.

Authors:  J E Mole; H R Horton
Journal:  Biochemistry       Date:  1973-02-27       Impact factor: 3.162

2.  Ionization behavior of the catalytic carboxyls of lysozyme.

Authors:  S M Parsons; M A Raftery
Journal:  Biochem Biophys Res Commun       Date:  1970-10-09       Impact factor: 3.575

3.  Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.

Authors:  K Brocklehurst; S M Brocklehurst; D Kowlessur; M O'Driscoll; G Patel; E Salih; W Templeton; E Thomas; C M Topham; F Willenbrock
Journal:  Biochem J       Date:  1988-12-01       Impact factor: 3.857

4.  Cloning and sequencing of papain-encoding cDNA.

Authors:  L W Cohen; V M Coghlan; L C Dihel
Journal:  Gene       Date:  1986       Impact factor: 3.688

5.  Thiol proteases. Comparative studies based on the high-resolution structures of papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain.

Authors:  I G Kamphuis; J Drenth; E N Baker
Journal:  J Mol Biol       Date:  1985-03-20       Impact factor: 5.469

6.  Calculation of the electric potential in the active site cleft due to alpha-helix dipoles.

Authors:  J Warwicker; H C Watson
Journal:  J Mol Biol       Date:  1982-06-05       Impact factor: 5.469

7.  Effects of conformational selectivity and of overlapping kinetically influential ionizations on the characteristics of pH-dependent enzyme kinetics. Implications of free-enzyme pKa variability in reactions of papain for its catalytic mechanism.

Authors:  K Brocklehurst; S J Willenbrock; E Salih
Journal:  Biochem J       Date:  1983-06-01       Impact factor: 3.857

8.  Structure of actinidin, after refinement at 1.7 A resolution.

Authors:  E N Baker
Journal:  J Mol Biol       Date:  1980-08-25       Impact factor: 5.469

9.  Chemical evidence for the pH-dependent control of ion-pair geometry in cathepsin B. Benzofuroxan as a reactivity probe sensitive to differences in the mutual disposition of the thiolate and imidazolium components of cysteine proteinase catalytic sites.

Authors:  F Willenbrock; K Brocklehurst
Journal:  Biochem J       Date:  1986-08-15       Impact factor: 3.857

10.  Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding.

Authors:  K Brocklehurst; D Kowlessur; M O'Driscoll; G Patel; S Quenby; E Salih; W Templeton; E W Thomas; F Willenbrock
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857
View more
  5 in total

1.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

2.  Structural and electrostatic differences between actinidin and papain account for differences in activity.

Authors:  R W Pickersgill; I G Sumner; M E Collins; P W Goodenough
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

3.  Variation in the pH-dependent pre-steady-state and steady-state kinetic characteristics of cysteine-proteinase mechanism: evidence for electrostatic modulation of catalytic-site function by the neighbouring carboxylate anion.

Authors:  Syeed Hussain; Surapong Pinitglang; Tamara S F Bailey; James D Reid; Michael A Noble; Marina Resmini; Emrys W Thomas; Richard B Greaves; Chandra S Verma; Keith Brocklehurst
Journal:  Biochem J       Date:  2003-06-15       Impact factor: 3.857

4.  Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.

Authors:  M P Thomas; C M Topham; D Kowlessur; G W Mellor; E W Thomas; D Whitford; K Brocklehurst
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

5.  Identification of Thermal Conduits That Link the Protein-Water Interface to the Active Site Loop and Catalytic Base in Enolase.

Authors:  Emily J Thompson; Adhayana Paul; Anthony T Iavarone; Judith P Klinman
Journal:  J Am Chem Soc       Date:  2021-01-04       Impact factor: 15.419
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.