Literature DB >> 2920024

Structural and electrostatic differences between actinidin and papain account for differences in activity.

R W Pickersgill, I G Sumner, M E Collins, P W Goodenough.   

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Year:  1989        PMID： 2920024      PMCID： PMC1135577          DOI： 10.1042/bj2570310

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


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  7 in total

1.  Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.

Authors:  E Salih; J P Malthouse; D Kowlessur; M Jarvis; M O'Driscoll; K Brocklehurst
Journal:  Biochem J       Date:  1987-10-01       Impact factor: 3.857

2.  The electrostatic fields in the active-site clefts of actinidin and papain.

Authors:  R W Pickersgill; P W Goodenough; I G Sumner; M E Collins
Journal:  Biochem J       Date:  1988-08-15       Impact factor: 3.857

3.  The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain.

Authors:  K Brocklehurst; M O'Driscoll; D Kowlessur; I R Phillips; W Templeton; E W Thomas; C M Topham; C W Wharton
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

4.  A rapid method of calculating charge-charge interaction energies in proteins.

Authors:  R W Pickersgill
Journal:  Protein Eng       Date:  1988-09

5.  Cloning and sequencing of papain-encoding cDNA.

Authors:  L W Cohen; V M Coghlan; L C Dihel
Journal:  Gene       Date:  1986       Impact factor: 3.688

6.  Structure of actinidin, after refinement at 1.7 A resolution.

Authors:  E N Baker
Journal:  J Mol Biol       Date:  1980-08-25       Impact factor: 5.469

7.  Structure of papain refined at 1.65 A resolution.

Authors:  I G Kamphuis; K H Kalk; M B Swarte; J Drenth
Journal:  J Mol Biol       Date:  1984-10-25       Impact factor: 5.469
  7 in total
  5 in total

1.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

2.  Ionization characteristics and chemical influences of aspartic acid residue 158 of papain and caricain determined by structure-related kinetic and computational techniques: multiple electrostatic modulators of active-centre chemistry.

Authors:  M A Noble; S Gul; C S Verma; K Brocklehurst
Journal:  Biochem J       Date:  2000-11-01       Impact factor: 3.857

3.  Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.

Authors:  G W Mellor; S K Sreedharan; D Kowlessur; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

4.  Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.

Authors:  M P Thomas; C M Topham; D Kowlessur; G W Mellor; E W Thomas; D Whitford; K Brocklehurst
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

5.  Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis.

Authors:  C M Topham; E Salih; C Frazao; D Kowlessur; J P Overington; M Thomas; S M Brocklehurst; M Patel; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1991-11-15       Impact factor: 3.857
  5 in total

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