| Literature DB >> 3023852 |
G Casey, R Smith, D McGillivray, G Peters, C Dickson.
Abstract
int-2 is one of two cellular genes (int-1 and int-2) currently implicated in the genesis of mammary carcinomas by mouse mammary tumor virus and may constitute a novel cellular proto-oncogene. Using low-stringency hybridization with mouse int-2 probes, we established that homologous genes exist in a variety of mammalian species, including humans, but failed to detect related sequences in other classes and phyla. Recombinant bacteriophage clones and a single cosmid encompassing the human int-2 gene were isolated and characterized by restriction enzyme mapping. A survey of nine primary human breast tumors, three breast tumor cell lines, and three normal individuals revealed no evidence for gross amplification or rearrangement of the int-2 locus. Three distinct restriction fragment length polymorphisms were observed which could prove useful in future linkage studies. By a combination of in situ hybridization of metaphase chromosomes and somatic cell genetics, the human int-2 gene was mapped to chromosome 11, band q13.Entities:
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Year: 1986 PMID： 3023852 PMCID： PMC367539 DOI： 10.1128/mcb.6.2.502-510.1986
Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272