Literature DB >> 6280136

Gene shuttling: moving of cloned DNA into and out of eukaryotic cells.

W Lindenmaier, H Hauser, I G de Wilke, G Schütz.   

Abstract

Successful shuttling of cloned DNA in eukaryotic cells should allow isolation of expressed genes. We tested the utility of cosmids for moving DNA into and out of eukaryotic cells. The unique cleavage of DNA at the cos site by the terminase function of lambda was exploited to maintain the linkage between the vector and inserted gene sequences, a prerequisite for successful rescue of the transforming DNA from high molecular weight DNA of the eukaryotic transformant. A cosmid recombinant containing the HSV thymidine kinase gene and a lambda recombinant containing the chicken thymidine kinase gene were used to test the feasability of this method. It was found that these recombinants can be rescued with high efficiency from DNA of HAT-resistant cells.

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Year:  1982        PMID： 6280136      PMCID： PMC320522          DOI： 10.1093/nar/10.4.1243

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Polarized packaging of bacteriophage lambda chromosomes.

Authors:  M Feiss; A Bublitz
Journal:  J Mol Biol       Date:  1975-06-05       Impact factor: 5.469

2.  Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

3.  Arrangement of coding and intervening sequences of chicken lysozyme gene.

Authors:  W Lindenmaier; M C Nguyen-Huu; R Lurz; M Stratmann; N Blin; T Wurtz; H J Hauser; A E Sippel; G Schütz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

4.  Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.

Authors:  B Cami; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

5.  Packaging of ColE1 DNA having a lambda phage cohesive end site.

Authors:  K Umene; K Shimada; Y Takagi
Journal:  Mol Gen Genet       Date:  1978-02-07

6.  On maturation of the bacteriophage lambda chromosome.

Authors:  M Feiss; T Margulies
Journal:  Mol Gen Genet       Date:  1973-12-31

7.  A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors:  L Clarke; J Carbon
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

8.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

9.  Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1.

Authors:  N M Wilkie; J B Clements; W Boll; N Mantei; D Lonsdale; C Weissmann
Journal:  Nucleic Acids Res       Date:  1979-10-25       Impact factor: 16.971

10.  Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage lambda: "cosmids".

Authors:  J Collins; H J Brüning
Journal:  Gene       Date:  1978-10       Impact factor: 3.688
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  21 in total

1.  Rescue of unstable cosmids by in vitro packaging.

Authors:  K Yokobata; B Trenchak; P J de Jong
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

2.  Characterization of the human 5-lipoxygenase gene.

Authors:  C D Funk; S Hoshiko; T Matsumoto; O Rdmark; B Samuelsson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

3.  Isolation and characterization of the human chorionic gonadotropin beta subunit (CG beta) gene cluster: regulation of transcriptionally active CG beta gene by cyclic AMP.

Authors:  J L Jameson; C M Lindell
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

4.  Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.

Authors:  P M Glazer; S N Sarkar; W C Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

5.  In vivo repackaging of recombinant cosmid molecules for analyses of Salmonella typhimurium, Streptococcus mutans, and mycobacterial genomic libraries.

Authors:  W R Jacobs; J F Barrett; J E Clark-Curtiss; R Curtiss
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

6.  Composite human VK genes and a model of their evolution.

Authors:  H R Jaenichen; M Pech; W Lindenmaier; N Wildgruber; H G Zachau
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

7.  Selective isolation of cosmid clones by homologous recombination in Escherichia coli.

Authors:  A Poustka; H R Rackwitz; A M Frischauf; B Hohn; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  Novel cluster of alpha-interferon gene sequences in a placental cosmid DNA library.

Authors:  B Lund; T Edlund; W Lindenmaier; T Ny; J Collins; E Lundgren; A von Gabain
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

9.  Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.

Authors:  H Schrewe; J Thompson; M Bona; L J Hefta; A Maruya; M Hassauer; J E Shively; S von Kleist; W Zimmermann
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

10.  The structure of the human tissue-type plasminogen activator gene: correlation of intron and exon structures to functional and structural domains.

Authors:  T Ny; F Elgh; B Lund
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205
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