| Literature DB >> 29334379 |
Daniel R Garalde1, Elizabeth A Snell1, Daniel Jachimowicz1, Botond Sipos1, Joseph H Lloyd1, Mark Bruce1, Nadia Pantic1, Tigist Admassu1, Phillip James1, Anthony Warland1, Michael Jordan1, Jonah Ciccone1, Sabrina Serra1, Jemma Keenan1, Samuel Martin1, Luke McNeill1, E Jayne Wallace1, Lakmal Jayasinghe1, Chris Wright1, Javier Blasco1, Stephen Young1, Denise Brocklebank1, Sissel Juul2, James Clarke1, Andrew J Heron1, Daniel J Turner1.
Abstract
Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.Mesh：
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Year: 2018 PMID： 29334379 DOI： 10.1038/nmeth.4577
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547