Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Genetic interaction mapping in mammalian cells using CRISPR interference.

Literature DB >> 28481362

Genetic interaction mapping in mammalian cells using CRISPR interference.

Dan Du^1,2,3, Assen Roguev^4,5, David E Gordon^4,5, Meng Chen^1,6, Si-Han Chen^4,5, Michael Shales^4,5, John Paul Shen^7,8, Trey Ideker^7,8, Prashant Mali⁸, Lei S Qi^1,2,3, Nevan J Krogan^4,5,6.

Abstract

We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein-protein-interaction data revealed a functional map of chromatin regulation.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Year: 2017 PMID： 28481362 PMCID： PMC5584685 DOI： 10.1038/nmeth.4286

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

27 in total

Review 1. RNA interference.

Authors: Gregory J Hannon
Journal: Nature Date: 2002-07-11 Impact factor: 49.962

2. Production of endoribonuclease-prepared short interfering RNAs for gene silencing in mammalian cells.

Authors: Ralf Kittler; Anne-Kristin Heninger; Kristin Franke; Bianca Habermann; Frank Buchholz
Journal: Nat Methods Date: 2005-10 Impact factor: 28.547

3. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking.

Authors: Pablo S Aguilar; Florian Fröhlich; Michael Rehman; Mike Shales; Igor Ulitsky; Agustina Olivera-Couto; Hannes Braberg; Ron Shamir; Peter Walter; Matthias Mann; Christer S Ejsing; Nevan J Krogan; Tobias C Walther
Journal: Nat Struct Mol Biol Date: 2010-06-06 Impact factor: 15.369

4. Quantitative genetic-interaction mapping in mammalian cells.

Authors: Assen Roguev; Dale Talbot; Gian Luca Negri; Michael Shales; Gerard Cagney; Sourav Bandyopadhyay; Barbara Panning; Nevan J Krogan
Journal: Nat Methods Date: 2013-02-13 Impact factor: 28.547

5. A lipid E-MAP identifies Ubx2 as a critical regulator of lipid saturation and lipid bilayer stress.

Authors: Michal A Surma; Christian Klose; Debby Peng; Michael Shales; Caroline Mrejen; Adam Stefanko; Hannes Braberg; David E Gordon; Daniela Vorkel; Christer S Ejsing; Robert Farese; Kai Simons; Nevan J Krogan; Robert Ernst
Journal: Mol Cell Date: 2013-07-25 Impact factor: 17.970

6. ASCOM controls farnesoid X receptor transactivation through its associated histone H3 lysine 4 methyltransferase activity.

Authors: Dae-Hwan Kim; Jeongkyung Lee; Bora Lee; Jae W Lee
Journal: Mol Endocrinol Date: 2009-06-25

7. A global genetic interaction network maps a wiring diagram of cellular function.

Authors: Michael Costanzo; Benjamin VanderSluis; Elizabeth N Koch; Anastasia Baryshnikova; Carles Pons; Guihong Tan; Wen Wang; Matej Usaj; Julia Hanchard; Susan D Lee; Vicent Pelechano; Erin B Styles; Maximilian Billmann; Jolanda van Leeuwen; Nydia van Dyk; Zhen-Yuan Lin; Elena Kuzmin; Justin Nelson; Jeff S Piotrowski; Tharan Srikumar; Sondra Bahr; Yiqun Chen; Raamesh Deshpande; Christoph F Kurat; Sheena C Li; Zhijian Li; Mojca Mattiazzi Usaj; Hiroki Okada; Natasha Pascoe; Bryan-Joseph San Luis; Sara Sharifpoor; Emira Shuteriqi; Scott W Simpkins; Jamie Snider; Harsha Garadi Suresh; Yizhao Tan; Hongwei Zhu; Noel Malod-Dognin; Vuk Janjic; Natasa Przulj; Olga G Troyanskaya; Igor Stagljar; Tian Xia; Yoshikazu Ohya; Anne-Claude Gingras; Brian Raught; Michael Boutros; Lars M Steinmetz; Claire L Moore; Adam P Rosebrock; Amy A Caudy; Chad L Myers; Brenda Andrews; Charles Boone
Journal: Science Date: 2016-09-23 Impact factor: 47.728

8. A strategy for extracting and analyzing large-scale quantitative epistatic interaction data.

Authors: Sean R Collins; Maya Schuldiner; Nevan J Krogan; Jonathan S Weissman
Journal: Genome Biol Date: 2006 Impact factor: 13.583

9. A map of directional genetic interactions in a metazoan cell.

Authors: Bernd Fischer; Thomas Sandmann; Thomas Horn; Maximilian Billmann; Varun Chaudhary; Wolfgang Huber; Michael Boutros
Journal: Elife Date: 2015-03-06 Impact factor: 8.140

10. Functional organization of the S. cerevisiae phosphorylation network.

Authors: Dorothea Fiedler; Hannes Braberg; Monika Mehta; Gal Chechik; Gerard Cagney; Paromita Mukherjee; Andrea C Silva; Michael Shales; Sean R Collins; Sake van Wageningen; Patrick Kemmeren; Frank C P Holstege; Jonathan S Weissman; Michael-Christopher Keogh; Daphne Koller; Kevan M Shokat; Nevan J Krogan
Journal: Cell Date: 2009-03-06 Impact factor: 41.582

54 in total

1. Unbiased Fitness Estimation of Pooled Barcode or Amplicon Sequencing Studies.

Authors: Fangfei Li; Marc L Salit; Sasha F Levy
Journal: Cell Syst Date: 2018-11-01 Impact factor: 10.304

2. Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.

Authors: Simone Rauch; Emily He; Michael Srienc; Huiqing Zhou; Zijie Zhang; Bryan C Dickinson
Journal: Cell Date: 2019-06-20 Impact factor: 41.582

Review 3. Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect.

Authors: Yu-Fan Chuang; Andrew J Phipps; Fan-Li Lin; Valerie Hecht; Alex W Hewitt; Peng-Yuan Wang; Guei-Sheung Liu
Journal: Cell Mol Life Sci Date: 2021-01-03 Impact factor: 9.261

4. A case of mistaken identity.

Authors: Ruth E Hanna; John G Doench
Journal: Nat Biotechnol Date: 2018-09-06 Impact factor: 54.908

Review 5. Genetic interaction networks in cancer cells.

Authors: Barbara Mair; Jason Moffat; Charles Boone; Brenda J Andrews
Journal: Curr Opin Genet Dev Date: 2019-04-08 Impact factor: 5.578

Review 6. Time for remodeling: SNF2-family DNA translocases in replication fork metabolism and human disease.

Authors: Sarah A Joseph; Angelo Taglialatela; Giuseppe Leuzzi; Jen-Wei Huang; Raquel Cuella-Martin; Alberto Ciccia
Journal: DNA Repair (Amst) Date: 2020-08-15

7. High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells.

Authors: Hui Kwon Kim; Sungtae Lee; Younggwang Kim; Jinman Park; Seonwoo Min; Jae Woo Choi; Tony P Huang; Sungroh Yoon; David R Liu; Hyongbum Henry Kim
Journal: Nat Biomed Eng Date: 2020-01-14 Impact factor: 25.671