| Literature DB >> 20526336 |
Pablo S Aguilar1, Florian Fröhlich, Michael Rehman, Mike Shales, Igor Ulitsky, Agustina Olivera-Couto, Hannes Braberg, Ron Shamir, Peter Walter, Matthias Mann, Christer S Ejsing, Nevan J Krogan, Tobias C Walther.
Abstract
The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.Entities:
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Year: 2010 PMID： 20526336 PMCID： PMC3073498 DOI： 10.1038/nsmb.1829
Source DB: PubMed Journal: Nat Struct Mol Biol ISSN： 1545-9985 Impact factor: 15.369