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Literature DB >> 284389

Human leukocyte interferon: production, purification to homogeneity, and initial characterization.

M Rubinstein, S Rubinstein, P C Familletti, R S Miller, A A Waldman, S Pestka.

Abstract

A method of fractionating proteins by high-performance liquid partition chromatography has been developed and used for isolation and purification to homogeneity of one of the species of human leukocyte interferon. The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Extraction of the gel gave a single sharp peak of antiviral activity coinciding with the protein band. The specific activity of pure interferon was found to be 2--4 X 10(8) units/mg, based on amino acid analysis. The molecular weight is 17,500--18,000.

Entities: Chemical Species

Mesh：

Substances：
Amino Acids
Interferons

Year: 1979 PMID： 284389 PMCID： PMC383004 DOI： 10.1073/pnas.76.2.640

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

32 in total

1. [Inhibition of vaccinia infection by a liquid factor in tissues infected by homologous virus].

Authors: Y NAGANO; Y KOJIMA
Journal: C R Seances Soc Biol Fil Date: 1958

2. Virus interference. II. Some properties of interferon.

Authors: A ISAACS; J LINDENMANN; R C VALENTINE
Journal: Proc R Soc Lond B Biol Sci Date: 1957-09-12

3. Virus interference. I. The interferon.

Authors: A ISAACS; J LINDENMANN
Journal: Proc R Soc Lond B Biol Sci Date: 1957-09-12

4. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

Authors: M Rubinstein; S Stein; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

5. Purification of two components of mouse L cell interferon: electrophoretic demonstration of interferon proteins.

Authors: Y Yamamoto; Y Kawade
Journal: J Gen Virol Date: 1976-11 Impact factor: 3.891

Review 6. Synthesis of interferon in heterologous cells, cell-free extracts, and Xenopus laevis oocytes.

Authors: R L Cavalieri; S Pestka
Journal: Tex Rep Biol Med Date: 1977

7. De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts.

Authors: P Tucker; S Pestka
Journal: J Biol Chem Date: 1977-07-10 Impact factor: 5.157

8. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

Authors: M Rubinstein; S Stein; L D Gerber; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

9. Purification of mouse interferon by sequential affinity chromatography on poly(U)--and antibody--agarose columns.

Authors: J De Maeyer-Guignard; M G Tovey; I Gresser; E De Maeyer
Journal: Nature Date: 1978-02-16 Impact factor: 49.962

10. Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.

Authors: R L Cavalieri; E A Havell; J Vilcek; S Pestka
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

39 in total

Review 1. Functions of the cytoplasmic RNA sensors RIG-I and MDA-5: key regulators of innate immunity.

Authors: Paola M Barral; Devanand Sarkar; Zao-zhong Su; Glen N Barber; Rob DeSalle; Vincent R Racaniello; Paul B Fisher
Journal: Pharmacol Ther Date: 2009-07-15 Impact factor: 12.310

2. Effects of recombinant leukocyte interferon on serum immunoglobulin concentrations and lymphocyte subpopulations in chronic hepatitis B.

Authors: H Tsuji; K Murai; K Akagi; M Fujishima
Journal: J Clin Immunol Date: 1990-01 Impact factor: 8.317

Human leukocyte interferon: production, purification to homogeneity, and initial characterization.

1. [Inhibition of vaccinia infection by a liquid factor in tissues infected by homologous virus].

2. Virus interference. II. Some properties of interferon.

3. Virus interference. I. The interferon.

4. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

5. Purification of two components of mouse L cell interferon: electrophoretic demonstration of interferon proteins.

Review 6. Synthesis of interferon in heterologous cells, cell-free extracts, and Xenopus laevis oocytes.

7. De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts.

8. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

9. Purification of mouse interferon by sequential affinity chromatography on poly(U)--and antibody--agarose columns.

10. Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.

Review 1. Functions of the cytoplasmic RNA sensors RIG-I and MDA-5: key regulators of innate immunity.

2. Effects of recombinant leukocyte interferon on serum immunoglobulin concentrations and lymphocyte subpopulations in chronic hepatitis B.

3. Purification and partial characterization of human lymphoblast interferon.

4. Molecular cloning of human interferon cDNA.

5. Amino-terminal amino acid sequence of human leukocyte interferon.

6. Glycosylation of human leukocyte interferon: effects of tunicamycin.

7. Human leukocyte interferon: relationship between molecular structure and species specificity.

Review 8. Induction of type I interferon by RNA viruses: cellular receptors and their substrates.

9. Sepharose-immobilized triazine dyes as adsorbants for human lymphoblastoid interferon purification.

10. Characterization of the bovine type I IFN locus: rearrangements, expansions, and novel subfamilies.