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Literature DB >> 270731

Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

Abstract

beta-Endorphin was isolated from 200 rat pituitaries by means of high-performance column chromatography, using sensitive fluorometric methods and a radioreceptor assay for opioid activity. The beta-endorphin was characterized as to molecular weight, amino acid composition, and mapping of tryptic peptides by a new microtechnique. It was found that rat beta-endorphin is identical to camel and sheep beta-endorphin. Furthermore, alpha-endorphin, Met-enkephalin, a nonpeptide morphine-like compound (MLC), and an additional unidentified opioid compound were detected in the extract of rat pituitary.

Entities: Chemical Species

Mesh：

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Year: 1977 PMID： 270731 PMCID： PMC432079 DOI： 10.1073/pnas.74.11.4969

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

6 in total

1. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

Authors: P Böhlen; S Stein; J Stone; S Udenfriend
Journal: Anal Biochem Date: 1975-08 Impact factor: 3.365

2. Antibodies as a means of isolating and characterizing biologically active substances: presence of a non-peptide, morphine-like compound in the central nervous system.

Authors: A R Gintzler; A Levy; S Spector
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

3. Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

Authors: C H Li; D Chung
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

4. Homology between a prolyl hydroxylase subunit and a tissue protein that crossreacts immunologically with the enzyme.

Authors: S Chen-Kiang; G J Cardinale; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

5. Amino acid analysis with fluorescamine at the picomole level.

Authors: S Stein; P Böhlen; J Stone; W Dairman; S Udenfriend
Journal: Arch Biochem Biophys Date: 1973-03 Impact factor: 4.013

6. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

Authors: M Rubinstein; S Stein; L D Gerber; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1977-07 Impact factor: 11.205

6 in total

13 in total

1. Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin.

Authors: S Kimura; R V Lewis; L D Gerber; L Brink; M Rubinstein; S Stein; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1979-04 Impact factor: 11.205

9. Isolation, characterization and opiate activity of beta-endorphin from the pituitary gland of the ostrich, Struthio camelus.

Authors: R J Naudé; W Oelofsen; R Maske
Journal: Biochem J Date: 1980-04-01 Impact factor: 3.857

10. Pituitary somatotrophs contain [Met]enkephalin-like immunoreactivity.

Authors: E Weber; K H Voigt; R Martin
Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

1. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

2. Antibodies as a means of isolating and characterizing biologically active substances: presence of a non-peptide, morphine-like compound in the central nervous system.

3. Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

4. Homology between a prolyl hydroxylase subunit and a tissue protein that crossreacts immunologically with the enzyme.

5. Amino acid analysis with fluorescamine at the picomole level.

6. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

1. Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin.

2. Isolation and characterization of beta-endorphin-like peptides from bovine brains.

3. Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin.

4. High molecular weight opioid-containing proteins in striatum.

5. Distribution of active and inactive forms of endorphins in rat pituitary and brain.

Review 6. Distribution of beta-endorphin-related peptides in rat pituitary and brain.

7. Prediction of peptide retention times in high-pressure liquid chromatography on the basis of amino acid composition.

8. Human leukocyte interferon: production, purification to homogeneity, and initial characterization.

9. Isolation, characterization and opiate activity of beta-endorphin from the pituitary gland of the ostrich, Struthio camelus.

10. Pituitary somatotrophs contain [Met]enkephalin-like immunoreactivity.