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Literature DB >> 342973

Purification of mouse interferon by sequential affinity chromatography on poly(U)--and antibody--agarose columns.

J De Maeyer-Guignard, M G Tovey, I Gresser, E De Maeyer.

Abstract

Mouse interferon has been purified to homogeneity by twostep affinity chromatography. Two polypeptide bands were obtained on sodium dodecyl sulphate--polyacrylamide gel electrophoresis migrating at molecular weights 35,000 and 22,000, both having antiviral activity. The 35,000 but not the 22,000 band, also stained with periodic acid--Schiff. The specific activity was 8 X 10(9) of our laboratory units, corresponding to 2.4 X 10(9) NIH reference units.

Entities: Chemical Species

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Year: 1978 PMID： 342973 DOI： 10.1038/271622a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

29 in total

1. Stable antiviral expression in BALB/c 3T3 cells carrying a beta interferon sequence behind a major histocompatibility complex promoter fragment.

Authors: I Seif; E De Maeyer; I Riviere; J De Maeyer-Guignard
Journal: J Virol Date: 1991-02 Impact factor: 5.103

2. Synthesis and secretion of interferon by murine fibroblasts in response to intracellular Listeria monocytogenes.

Authors: E A Havell
Journal: Infect Immun Date: 1986-12 Impact factor: 3.441

3. Interferoids: in vitro and in vivo conversion of native interferons to lower molecular weight forms.

Authors: W E Stewart; T Chudzio; L S Lin; M Wiranowska-Stewart
Journal: Proc Natl Acad Sci U S A Date: 1978-10 Impact factor: 11.205

Review 4. Interferon receptors.

Authors: A A Branca
Journal: In Vitro Cell Dev Biol Date: 1988-03

10. Heterogeneity of interferon mRNA species from Sendai virus-induced human lymphoblastoid (Namalva) cells and Newcastle disease virus-induced murine fibroblastoid (L) cells.

Authors: A D Sagar; L A Pickering; P Sussman-Berger; W E Stewart; P B Sehgal
Journal: Nucleic Acids Res Date: 1981-01-10 Impact factor: 16.971

Purification of mouse interferon by sequential affinity chromatography on poly(U)--and antibody--agarose columns.

1. Stable antiviral expression in BALB/c 3T3 cells carrying a beta interferon sequence behind a major histocompatibility complex promoter fragment.

2. Synthesis and secretion of interferon by murine fibroblasts in response to intracellular Listeria monocytogenes.

3. Interferoids: in vitro and in vivo conversion of native interferons to lower molecular weight forms.

Review 4. Interferon receptors.

5. Inhibition of herpes simplex virus type 2-induced biochemical transformation by interferon.

6. The messenger RNA sequences in human fibroblast cells induced with poly rI.rC to produce interferon.

7. Electrophoretically pure mouse interferon exerts multiple biologic effects.

8. Human leukocyte interferon: relationship between molecular structure and species specificity.

9. Electrophoretically pure mouse interferon inhibits growth, induces liver and kidney lesions, and kills suckling mice.

10. Heterogeneity of interferon mRNA species from Sendai virus-induced human lymphoblastoid (Namalva) cells and Newcastle disease virus-induced murine fibroblastoid (L) cells.