Literature DB >> 2843147

An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.

H Puchta1, H L Sänger.   

Abstract

The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multi-ion transformation is applied.

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Year:  1988        PMID： 2843147     DOI： 10.1007/BF01314659

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  12 in total

1.  Mutational analysis of potato spindle tuber viroid reveals complex relationships between structure and infectivity.

Authors:  R W Hammond; R A Owens
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

2.  Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization.

Authors:  R A Owens; T O Diener
Journal:  Science       Date:  1981-08-07       Impact factor: 47.728

3.  Molecular cloning of potato spindle tuber viroid (PSTV) cDNA synthesized by enzymatic elongation of PSTV-specific DNA primers: a general strategy for viroid cloning.

Authors:  M Tabler; M Schnölzer; H L Sänger
Journal:  Biosci Rep       Date:  1985-02       Impact factor: 3.840

4.  Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity.

Authors:  J E Visvader; R H Symons
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

5.  Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid.

Authors:  T Sano; I Uyeda; E Shikata; T Ohno; Y Okada
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971

6.  The molecular structure of hop latent viroid (HLV), a new viroid occurring worldwide in hops.

Authors:  H Puchta; K Ramm; H L Sänger
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971

7.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

8.  Construction of infectious potato spindle tuber viroid cDNA clones.

Authors:  D E Cress; M C Kiefer; R A Owens
Journal:  Nucleic Acids Res       Date:  1983-10-11       Impact factor: 16.971

9.  Infectivity studies on different potato spindle tuber viroid (PSTV) RNAs synthesized in vitro with the SP6 transcription system.

Authors:  M Tabler; H L Sänger
Journal:  EMBO J       Date:  1985-09       Impact factor: 11.598

10.  Linear oligomeric potato spindle tuber viroid (PSTV) RNAs are accurately processed in vitro to the monomeric circular viroid proper when incubated with a nuclear extract from healthy potato cells.

Authors:  M Tsagris; M Tabler; H P Mühlbach; H L Sänger
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
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  3 in total

1.  Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication.

Authors:  H Puchta; H L Sänger
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

2.  Analysis of the population structure of three phenotypically different PSTVd isolates.

Authors:  A Góra; T Candresse; W Zagórski
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

3.  Primary and secondary structure of a 360-nucleotide isolate of potato spindle tuber viroid.

Authors:  D K Lakshman; S M Tavantzis
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574
  3 in total

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