Literature DB >> 2476110

Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication.

H Puchta1, H L Sänger.   

Abstract

The amount of viroid RNA required for sequence analysis can be reduced by five to six orders of magnitude when a modified "polymerase chain reaction" (PCR) is used for the amplification of the reversely transcribed, overlapping viroid cDNAs. By applying this procedure it is possible to establish the molecular structure also of those viroids which are present only in extremely low amounts in various crop plants and ornamentals.

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Year:  1989        PMID： 2476110     DOI： 10.1007/bf01313962

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  15 in total

1.  Complete nucleotide sequence of a viroid isolated from Etrog citron, a new member of hop stunt viroid group.

Authors:  T Sano; T Hataya; E Shikata
Journal:  Nucleic Acids Res       Date:  1988-01-11       Impact factor: 16.971

2.  Nucleotide sequence of a hop stunt viroid isolate from the German grapevine cultivar 'Riesling'.

Authors:  H Puchta; K Ramm; H L Sänger
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

3.  An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.

Authors:  H Puchta; H L Sänger
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

4.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

5.  Isolation of three viroids and a circular RNA from grapevines.

Authors:  M A Rezaian; A M Koltunow; L R Krake
Journal:  J Gen Virol       Date:  1988-02       Impact factor: 3.891

6.  How to build an inexpensive cyclotherm instrument for automated polymerase chain reaction.

Authors:  M Collasius; H Falk; C Ciesler; G Valet
Journal:  Anal Biochem       Date:  1989-08-15       Impact factor: 3.365

7.  A two-dimensional electrophoretic technique for the detection of circular viroids and virusoids.

Authors:  J Schumacher; J W Randles; D Riesner
Journal:  Anal Biochem       Date:  1983-12       Impact factor: 3.365

8.  A microscale procedure for isolating and sequencing the viroid RNA present in one gram of infected leaf tissue.

Authors:  M Tabler; I Günther; R Kern; H L Sänger
Journal:  J Virol Methods       Date:  1989-02       Impact factor: 2.014

9.  Nucleotide sequence of a hop stunt viroid (HSVd) isolate from the German grapevine rootstock 5BB as determined by PCR-mediated sequence analysis.

Authors:  H Puchta; K Ramm; R Luckinger; K Freimüller; H L Sänger
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

10.  The sequence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid.

Authors:  F García-Arenal; V Pallás; R Flores
Journal:  Nucleic Acids Res       Date:  1987-05-26       Impact factor: 16.971
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  4 in total

1.  Sequencing of double-stranded PCR products.

Authors:  S Gal
Journal:  Mol Biotechnol       Date:  1996-04       Impact factor: 2.695

2.  Direct sequencing of double-stranded DNA PCR products via removing the complementary strand with single-stranded DNA of an M13 clone.

Authors:  S Gal; B Hohn
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

3.  A new method for designing PCR primers specific for groups of sequences and its application to plant viruses.

Authors:  J Antoniw
Journal:  Mol Biotechnol       Date:  1995-10       Impact factor: 2.695

4.  An appraisal of different methods for the detection of the walnut strain of cherry leafroll virus.

Authors:  M J Borja; F Ponz
Journal:  J Virol Methods       Date:  1992-01       Impact factor: 2.014
  4 in total

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