| Literature DB >> 27865050 |
Jan Stanek1, Loren B Andreas1, Kristaps Jaudzems1, Diane Cala1, Daniela Lalli1, Andrea Bertarello1, Tobias Schubeis1, Inara Akopjana2, Svetlana Kotelovica2, Kaspars Tars2, Andrea Pica3, Serena Leone3, Delia Picone3, Zhi-Qiang Xu4, Nicholas E Dixon4, Denis Martinez5, Mélanie Berbon5, Nadia El Mammeri5, Abdelmajid Noubhani5, Sven Saupe6, Birgit Habenstein5, Antoine Loquet5, Guido Pintacuda1.
Abstract
We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid-state NMR spectroscopy with 100-111 kHz magic-angle spinning (MAS). The excellent resolution in the Cα-Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, a capsid and amyloid fibrils. A set of 3D spectra based on a Cα-Hα detection block was developed and applied for the sequence-specific backbone and aliphatic side-chain resonance assignment using only 500 μg of sample. These developments accelerate structural studies of biomolecular assemblies available in submilligram quantities without the need of protein deuteration.Entities:
Keywords: magic-angle spinning; proton detection; resonance assignment; solid-state NMR spectroscopy
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Year: 2016 PMID： 27865050 DOI： 10.1002/anie.201607084
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336