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Literature DB >> 27701014

MicroED opens a new era for biological structure determination.

Abstract

In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.

Entities: Chemical

Mesh：

Substances：
Proteins

Year: 2016 PMID： 27701014 PMCID： PMC5656569 DOI： 10.1016/j.sbi.2016.09.007

Source DB: PubMed Journal: Curr Opin Struct Biol ISSN： 0959-440X Impact factor: 6.809

50 in total

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Journal: Methods Mol Biol Date: 2007

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Journal: Proc Natl Acad Sci U S A Date: 2015-02-17 Impact factor: 11.205

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Journal: Acta Crystallogr D Biol Crystallogr Date: 2011-03-18

9. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.

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Authors: Tamir Gonen
Journal: Methods Mol Biol Date: 2013

16 in total

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Journal: Methods Date: 2017-05-12 Impact factor: 3.608

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Journal: Nat Methods Date: 2019-04-29 Impact factor: 28.547

Review 5. The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.

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6. Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights.

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Journal: Acta Crystallogr F Struct Biol Commun Date: 2016-11-25 Impact factor: 1.056