| Literature DB >> 20887851 |
Priyanka D Abeyrathne1, Mohamed Chami, Radosav S Pantelic, Kenneth N Goldie, Henning Stahlberg.
Abstract
Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close to native conditions. In this review, we discuss the factors of sample preparation that are key to elucidating the atomic structure of membrane proteins using electron crystallography.Mesh：
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Year: 2010 PMID： 20887851 DOI： 10.1016/S0076-6879(10)81001-8
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600