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Literature DB >> 27554600

Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.

Piyush K Jain¹, Vyas Ramanan¹, Arnout G Schepers¹, Nisha S Dalvie², Apekshya Panda², Heather E Fleming¹, Sangeeta N Bhatia^3,4,5,6,7.

Abstract

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

Entities: CellLine Chemical Disease Gene Species

Keywords: CRISPR; DNA cleavage; gene technology; nucleic acids; photochemistry

Mesh：

Substances：

Year: 2016 PMID： 27554600 PMCID： PMC5864249 DOI： 10.1002/anie.201606123

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

30 in total

Review 1. Regulating gene expression with light-activated oligonucleotides.

Authors: XinJing Tang; Ivan J Dmochowski
Journal: Mol Biosyst Date: 2006-11-20

2. Light-controlled gene silencing in zebrafish embryos.

Authors: Ilya A Shestopalov; Surajit Sinha; James K Chen
Journal: Nat Chem Biol Date: 2007-08-23 Impact factor: 15.040

3. Optical Control of CRISPR/Cas9 Gene Editing.

Authors: James Hemphill; Erin K Borchardt; Kalyn Brown; Aravind Asokan; Alexander Deiters
Journal: J Am Chem Soc Date: 2015-04-23 Impact factor: 15.419

4. A split-Cas9 architecture for inducible genome editing and transcription modulation.

Authors: Bernd Zetsche; Sara E Volz; Feng Zhang
Journal: Nat Biotechnol Date: 2015-02 Impact factor: 54.908

5. The role of CD33 as therapeutic target in acute myeloid leukemia.

Authors: Roland B Walter
Journal: Expert Opin Ther Targets Date: 2014-04-21 Impact factor: 6.902

Review 6. Genome editing. The new frontier of genome engineering with CRISPR-Cas9.

Authors: Jennifer A Doudna; Emmanuelle Charpentier
Journal: Science Date: 2014-11-28 Impact factor: 47.728

7. Genome engineering using the CRISPR-Cas9 system.

Authors: F Ann Ran; Patrick D Hsu; Jason Wright; Vineeta Agarwala; David A Scott; Feng Zhang
Journal: Nat Protoc Date: 2013-10-24 Impact factor: 13.491

8. Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia.

Authors: Qian Liu; Mangju Wang; Yang Hu; Haizhou Xing; Xue Chen; Ying Zhang; Ping Zhu
Journal: Leuk Lymphoma Date: 2013-08-20

9. Photoactivated Spatiotemporally-Responsive Nanosensors of in Vivo Protease Activity.

Authors: Jaideep S Dudani; Piyush K Jain; Gabriel A Kwong; Kelly R Stevens; Sangeeta N Bhatia
Journal: ACS Nano Date: 2015-11-13 Impact factor: 15.881

10. In vivo genome editing using Staphylococcus aureus Cas9.

Authors: F Ann Ran; Le Cong; Winston X Yan; David A Scott; Jonathan S Gootenberg; Andrea J Kriz; Bernd Zetsche; Ophir Shalem; Xuebing Wu; Kira S Makarova; Eugene V Koonin; Phillip A Sharp; Feng Zhang
Journal: Nature Date: 2015-04-01 Impact factor: 49.962

38 in total

Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.

Review 1. Regulating gene expression with light-activated oligonucleotides.

2. Light-controlled gene silencing in zebrafish embryos.

3. Optical Control of CRISPR/Cas9 Gene Editing.

4. A split-Cas9 architecture for inducible genome editing and transcription modulation.

5. The role of CD33 as therapeutic target in acute myeloid leukemia.

Review 6. Genome editing. The new frontier of genome engineering with CRISPR-Cas9.

7. Genome engineering using the CRISPR-Cas9 system.

8. Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia.

9. Photoactivated Spatiotemporally-Responsive Nanosensors of in Vivo Protease Activity.

10. In vivo genome editing using Staphylococcus aureus Cas9.

1. Very fast CRISPR on demand.

2. Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light-Activated Guide RNA.

Review 3. Optochemical Control of Biological Processes in Cells and Animals.

4. A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9.

5. Diffusion of DNA-Binding Species in the Nucleus: A Transient Anomalous Subdiffusion Model.

6. Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing.

7. Rational design of semiconducting polymer brushes as cancer theranostics.

8. Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNA^Gln-processing system in human cells.

Review 9. Precision Control of CRISPR-Cas9 Using Small Molecules and Light.

10. Efficient Synthesis of Light-Triggered Circular Antisense Oligonucleotides Targeting Cellular Protein Expression.