Literature DB >> 27121590

Sparse (13)C labelling for solid-state NMR studies of P. pastoris expressed eukaryotic seven-transmembrane proteins.

Jing Liu1,2,3, Chang Liu2, Ying Fan4,5, Rachel A Munro5,6, Vladimir Ladizhansky5,6, Leonid S Brown5,6, Shenlin Wang7,8,9.   

Abstract

We demonstrate a novel sparse (13)C labelling approach for methylotrophic yeast P. pastoris expression system, towards solid-state NMR studies of eukaryotic membrane proteins. The labelling scheme was achieved by co-utilizing natural abundance methanol and specifically (13)C labelled glycerol as carbon sources in the expression medium. This strategy improves the spectral resolution by 1.5 fold, displays site-specific labelling patterns, and has advantages for collecting long-range distance restraints for structure determination of large eukaryotic membrane proteins by solid-state NMR.

Entities:  

Keywords:  Eukaryotic seven-transmembrane proteins; Magic-angle-spinning solid-state NMR; P. pastoris expression system; Sparse 13C isotope labelling

Mesh：

Substances：

Year:  2016        PMID： 27121590     DOI： 10.1007/s10858-016-0033-1

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  35 in total

1.  Selective and extensive 13C labeling of a membrane protein for solid-state NMR investigations.

Authors:  M Hong; K Jakes
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

2.  High-resolution membrane protein structure by joint calculations with solid-state NMR and X-ray experimental data.

Authors:  Ming Tang; Lindsay J Sperling; Deborah A Berthold; Charles D Schwieters; Anna E Nesbitt; Andrew J Nieuwkoop; Robert B Gennis; Chad M Rienstra
Journal:  J Biomol NMR       Date:  2011-09-22       Impact factor: 2.835

3.  Cellular solid-state nuclear magnetic resonance spectroscopy.

Authors:  Marie Renault; Ria Tommassen-van Boxtel; Martine P Bos; Jan Andries Post; Jan Tommassen; Marc Baldus
Journal:  Proc Natl Acad Sci U S A       Date:  2012-02-13       Impact factor: 11.205

4.  Paramagnetic shifts in solid-state NMR of proteins to elicit structural information.

Authors:  Stéphane Balayssac; Ivano Bertini; Anusarka Bhaumik; Moreno Lelli; Claudio Luchinat
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-06       Impact factor: 11.205

5.  Membrane-protein structure determination by solid-state NMR spectroscopy of microcrystals.

Authors:  Shakeel Ahmad Shahid; Benjamin Bardiaux; W Trent Franks; Ludwig Krabben; Michael Habeck; Barth-Jan van Rossum; Dirk Linke
Journal:  Nat Methods       Date:  2012-11-11       Impact factor: 28.547

6.  Solid-state NMR and SAXS studies provide a structural basis for the activation of alphaB-crystallin oligomers.

Authors:  Stefan Jehle; Ponni Rajagopal; Benjamin Bardiaux; Stefan Markovic; Ronald Kühne; Joseph R Stout; Victoria A Higman; Rachel E Klevit; Barth-Jan van Rossum; Hartmut Oschkinat
Journal:  Nat Struct Mol Biol       Date:  2010-08-29       Impact factor: 15.369

7.  Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra.

Authors:  Sanaz Emami; Ying Fan; Rachel Munro; Vladimir Ladizhansky; Leonid S Brown
Journal:  J Biomol NMR       Date:  2013-01-24       Impact factor: 2.835

8.  Molecular structure of β-amyloid fibrils in Alzheimer's disease brain tissue.

Authors:  Jun-Xia Lu; Wei Qiang; Wai-Ming Yau; Charles D Schwieters; Stephen C Meredith; Robert Tycko
Journal:  Cell       Date:  2013-09-12       Impact factor: 41.582

9.  Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.

Authors:  Oriol Cos; Ramón Ramón; José Luis Montesinos; Francisco Valero
Journal:  Microb Cell Fact       Date:  2006-04-06       Impact factor: 5.328

10.  Structure of the chemokine receptor CXCR1 in phospholipid bilayers.

Authors:  Sang Ho Park; Bibhuti B Das; Fabio Casagrande; Ye Tian; Henry J Nothnagel; Mignon Chu; Hans Kiefer; Klaus Maier; Anna A De Angelis; Francesca M Marassi; Stanley J Opella
Journal:  Nature       Date:  2012-10-21       Impact factor: 49.962
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  6 in total

Review 1.  On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies.

Authors:  Lindsay Clark; Igor Dikiy; Daniel M Rosenbaum; Kevin H Gardner
Journal:  J Biomol NMR       Date:  2018-08-18       Impact factor: 2.835

2.  Enhancing sensitivity of Double Electron-Electron Resonance (DEER) by using Relaxation-Optimized Acquisition Length Distribution (RELOAD) scheme.

Authors:  Sergey Milikisiyants; Maxim A Voinov; Antonin Marek; Morteza Jafarabadi; Jing Liu; Rong Han; Shenlin Wang; Alex I Smirnov
Journal:  J Magn Reson       Date:  2018-12-05       Impact factor: 2.229

3.  Gd3+-chelated lipid accelerates solid-state NMR spectroscopy of seven-transmembrane proteins.

Authors:  Chang Liu; Jing Liu; Xiaojun Xu; ShengQi Xiang; Shenlin Wang
Journal:  J Biomol NMR       Date:  2017-05-30       Impact factor: 2.835

4.  Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system.

Authors:  Rika Suzuki; Masayoshi Sakakura; Masaki Mori; Moe Fujii; Satoko Akashi; Hideo Takahashi
Journal:  J Biomol NMR       Date:  2018-06-05       Impact factor: 2.835

5.  Codon Harmonization of a Kir3.1-KirBac1.3 Chimera for Structural Study Optimization.

Authors:  Evan van Aalst; Maryam Yekefallah; Anil K Mehta; Isaac Eason; Benjamin Wylie
Journal:  Biomolecules       Date:  2020-03-10

6.  Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in Pichia pastoris for Solid-State NMR.

Authors:  Rachel Munro; Jeffrey de Vlugt; Vladimir Ladizhansky; Leonid S Brown
Journal:  Biomolecules       Date:  2020-03-11
  6 in total

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