Literature DB >> 2663916

Comparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis.

W LeBar1, B Herschman, C Jemal, J Pierzchala.   

Abstract

A total of 201 endocervical specimens were obtained from patients with a clinical or epidemiological history suggestive of chlamydial infection. These specimens were tested by DNA probe (Gen-Probe, San Diego, Calif.) and the IDEIA III (Boots-Celltech, Berkshire, United Kingdom) monoclonal antibody enzyme immunoassay and compared with cell culture for detection of Chlamydia trachomatis. Discrepancies between cell culture and antigen detection methods were resolved by direct fluorescent-antibody testing. In a population with a 17.4% prevalence, the sensitivities and specificities of these assays were 82.8 and 99.4%, respectively, for the DNA probe assay and 97.1 and 98.1%, respectively, for the IDEIA III.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2663916      PMCID： PMC267437          DOI： 10.1128/jcm.27.5.826-828.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Rapid diagnosis of sexually transmitted diseases--speed has a price.

Authors:  J Schachter
Journal:  Diagn Microbiol Infect Dis       Date:  1986-03       Impact factor: 2.803

2.  Comparison of three techniques for detection of Chlamydia trachomatis in endocervical specimens from asymptomatic women.

Authors:  J Lefebvre; H Laperrière; H Rousseau; R Massé
Journal:  J Clin Microbiol       Date:  1988-04       Impact factor: 5.948

3.  Immunocytochemical demonstration of Chlamydia infection in the urogenital tracts.

Authors:  T K Kobayashi; M Ueda; H Araki; K Toyoda; K Ohmori; I Sawaragi
Journal:  Diagn Cytopathol       Date:  1987-12       Impact factor: 1.582

4.  Comparison of two enzyme immunoassays and an immunofluorescence test for detection of Chlamydia trachomatis.

Authors:  L Grillner; S Beckman; H Hammar
Journal:  Eur J Clin Microbiol       Date:  1986-10       Impact factor: 3.267

5.  Rapid detection of Chlamydia trachomatis by an enzyme immunoassay method.

Authors:  R W Ryan; I Kwasnik; O Steingrimsson; J Gudmundsson; H Thorarinsson; R C Tilton
Journal:  Diagn Microbiol Infect Dis       Date:  1986-09       Impact factor: 2.803

6.  Enzyme amplified immunoassay: a novel technique applied to direct detection of Chlamydia trachomatis in clinical specimens.

Authors:  S F Pugh; R C Slack; E O Caul; I D Paul; P N Appleton; S Gatley
Journal:  J Clin Pathol       Date:  1985-10       Impact factor: 3.411

7.  Use of a direct fluorescent antibody test for detecting Chlamydia trachomatis cervical infection in women seeking routine gynecologic care.

Authors:  R S Phillips; P A Hanff; R S Kauffman; M D Aronson
Journal:  J Infect Dis       Date:  1987-10       Impact factor: 5.226

8.  Evaluation of an enzyme immunoassay for the diagnosis of chlamydial infections in urogenital specimens.

Authors:  K H Tjiam; B Y van Heijst; A van Zuuren; J H Wagenvoort; T van Joost; E Stolz; M F Michel
Journal:  J Clin Microbiol       Date:  1986-04       Impact factor: 5.948

9.  Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics.

Authors:  J W Smith; R E Rogers; B P Katz; J F Brickler; P L Lineback; B Van der Pol; R B Jones
Journal:  J Clin Microbiol       Date:  1987-05       Impact factor: 5.948

10.  Immunodiagnosis of sexually transmitted disease.

Authors:  J Schachter
Journal:  Yale J Biol Med       Date:  1985 Sep-Oct
View more
  19 in total

1.  Confirmatory polymerase chain reaction testing for Chlamydia trachomatis in first-void urine from asymptomatic and symptomatic men.

Authors:  J B Mahony; K E Luinstra; J W Sellors; D Jang; M A Chernesky
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

Review 2.  Molecular techniques in the diagnosis of human infectious diseases.

Authors:  U Desselberger; K Collingham
Journal:  Genitourin Med       Date:  1990-10

Review 3.  Molecular biology and respiratory disease. 6. Modern molecular biology and respiratory bacterial infections: a revolution on the horizon.

Authors:  T F Murphy
Journal:  Thorax       Date:  1990-07       Impact factor: 9.139

Review 4.  Laboratory techniques for the diagnosis of chlamydial infections.

Authors:  D Taylor-Robinson; B J Thomas
Journal:  Genitourin Med       Date:  1991-06

5.  Evaluation of acridinium-ester-labeled DNA probes for identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture.

Authors:  M Goto; S Oka; K Okuzumi; S Kimura; K Shimada
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

6.  Comparison of IDEIA III and cell culture for the detection of Chlamydia trachomatis in endocervical specimens.

Authors:  W D Lebar; H Schubiner; C Jemal; B R Herschman
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

7.  Performance characteristics of the Gen-Probe Probe Competition Assay used as a supplementary test for the Gen-Probe PACE 2 and 2C assays for detection of Chlamydia trachomatis.

Authors:  J L Beebe; T R Sharpton; S N Zanto; R S Steece; C Rogers; S L Mottice
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

8.  Evaluation of Syva enzyme immunoassay for detection of Chlamydia trachomatis in genital specimens.

Authors:  C A Gaydos; C A Reichart; J M Long; L E Welsh; T M Neumann; E W Hook; T C Quinn
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

9.  Evaluation of two rapid tests for the diagnosis of Chlamydia trachomatis genital infections.

Authors:  H H Schubiner; W D LeBar; S Joseph; C Taylor; C Jemal
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-06       Impact factor: 3.267

10.  Detection of Chlamydia trachomatis in urethral and urine samples from symptomatic and asymptomatic male patients by the polymerase chain reaction.

Authors:  A Stary; B Choueiri; I Hörting-Müller; P Halisch; L Teodorowicz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-06       Impact factor: 3.267
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.