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Literature DB >> 25625321

Inteins as traceless purification tags for unnatural amino acid proteins.

Solongo Batjargal¹, Christopher R Walters, E James Petersson.

Abstract

The expression of proteins containing unnatural amino acids through suppression of a stop codon can be limited by truncation due to competition with release factors. When the site of incorporation is near the C-terminus, it may not be feasible to separate the full-length unnatural amino acid protein from the truncated form. We report a simple, traceless procedure that allows one to isolate the desired protein using a C-terminal intein fusion.

Entities: CellLine Chemical Disease Gene Species

Mesh：

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Year: 2015 PMID： 25625321 PMCID： PMC4473854 DOI： 10.1021/ja5103019

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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6. ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference.

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