| Literature DB >> 30657670 |
Joomyung V Jun1, Conor M Haney1, Richard J Karpowicz2, Sam Giannakoulias1, Virginia M-Y Lee2, E James Petersson1, David M Chenoweth1.
Abstract
Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide-alkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules.Entities:
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Year: 2019 PMID： 30657670 PMCID： PMC6732186 DOI： 10.1021/jacs.8b13094
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419