Literature DB >> 24136966

Genomically recoded organisms expand biological functions.

Marc J Lajoie1, Alexis J Rovner, Daniel B Goodman, Hans-Rudolf Aerni, Adrian D Haimovich, Gleb Kuznetsov, Jaron A Mercer, Harris H Wang, Peter A Carr, Joshua A Mosberg, Nadin Rohland, Peter G Schultz, Joseph M Jacobson, Jesse Rinehart, George M Church, Farren J Isaacs.   

Abstract

We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.

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Year:  2013        PMID： 24136966      PMCID： PMC4924538          DOI： 10.1126/science.1241459

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  25 in total

1.  Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code.

Authors:  J D Bain; C Switzer; A R Chamberlin; S A Benner
Journal:  Nature       Date:  1992-04-09       Impact factor: 49.962

2.  Red queen dynamics of protein translation.

Authors:  David C Krakauer; Vincent A A Jansen
Journal:  J Theor Biol       Date:  2002-09-07       Impact factor: 2.691

3.  Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome.

Authors:  Heinz Neumann; Kaihang Wang; Lloyd Davis; Maria Garcia-Alai; Jason W Chin
Journal:  Nature       Date:  2010-02-14       Impact factor: 49.962

Review 4.  Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.

Authors:  G Eggertsson; D Söll
Journal:  Microbiol Rev       Date:  1988-09

5.  Expanding the genetic code of Escherichia coli with phosphoserine.

Authors:  Hee-Sung Park; Michael J Hohn; Takuya Umehara; Li-Tao Guo; Edith M Osborne; Jack Benner; Christopher J Noren; Jesse Rinehart; Dieter Söll
Journal:  Science       Date:  2011-08-26       Impact factor: 47.728

6.  An expanded genetic code with a functional quadruplet codon.

Authors:  J Christopher Anderson; Ning Wu; Stephen W Santoro; Vishva Lakshman; David S King; Peter G Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-11       Impact factor: 11.205

7.  Programming cells by multiplex genome engineering and accelerated evolution.

Authors:  Harris H Wang; Farren J Isaacs; Peter A Carr; Zachary Z Sun; George Xu; Craig R Forest; George M Church
Journal:  Nature       Date:  2009-07-26       Impact factor: 49.962

8.  Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection.

Authors:  Peter A Carr; Harris H Wang; Bram Sterling; Farren J Isaacs; Marc J Lajoie; George Xu; George M Church; Joseph M Jacobson
Journal:  Nucleic Acids Res       Date:  2012-05-25       Impact factor: 16.971

9.  The establishment of genetically engineered canola populations in the U.S.

Authors:  Meredith G Schafer; Andrew A Ross; Jason P Londo; Connie A Burdick; E Henry Lee; Steven E Travers; Peter K Van de Water; Cynthia L Sagers
Journal:  PLoS One       Date:  2011-10-05       Impact factor: 3.240

10.  RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites.

Authors:  David B F Johnson; Jianfeng Xu; Zhouxin Shen; Jeffrey K Takimoto; Matthew D Schultz; Robert J Schmitz; Zheng Xiang; Joseph R Ecker; Steven P Briggs; Lei Wang
Journal:  Nat Chem Biol       Date:  2011-09-18       Impact factor: 15.040
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  274 in total

1.  Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation.

Authors:  Daniel Rösner; Tatjana Schneider; Daniel Schneider; Martin Scheffner; Andreas Marx
Journal:  Nat Protoc       Date:  2015-09-24       Impact factor: 13.491

Review 2.  Recent advances and versatility of MAGE towards industrial applications.

Authors:  Vijai Singh; Darren Braddick
Journal:  Syst Synth Biol       Date:  2015-11-07

3.  Addicting diverse bacteria to a noncanonical amino acid.

Authors:  Drew S Tack; Jared W Ellefson; Ross Thyer; Bo Wang; Jimmy Gollihar; Matthew T Forster; Andrew D Ellington
Journal:  Nat Chem Biol       Date:  2016-01-18       Impact factor: 15.040

4.  Systematic Evaluation of Soluble Protein Expression Using a Fluorescent Unnatural Amino Acid Reveals No Reliable Predictors of Tolerability.

Authors:  Zachary M Hostetler; John J Ferrie; Marc R Bornstein; Itthipol Sungwienwong; E James Petersson; Rahul M Kohli
Journal:  ACS Chem Biol       Date:  2018-09-20       Impact factor: 5.100

5.  Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads.

Authors:  J A Moore; M Nemat-Gorgani; A C Madison; M A Sandahl; S Punnamaraju; A E Eckhardt; M G Pollack; F Vigneault; G M Church; R B Fair; M A Horowitz; P B Griffin
Journal:  Biomicrofluidics       Date:  2017-02-03       Impact factor: 2.800

6.  Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation.

Authors:  Qing Cheng; Elias S J Arnér
Journal:  J Biol Chem       Date:  2017-02-13       Impact factor: 5.157

Review 7.  Semisynthetic Organisms with Expanded Genetic Codes.

Authors:  Yorke Zhang; Floyd E Romesberg
Journal:  Biochemistry       Date:  2018-04-06       Impact factor: 3.162

8.  FFAT motif phosphorylation controls formation and lipid transfer function of inter-organelle contacts.

Authors:  Thomas Di Mattia; Arthur Martinet; Souade Ikhlef; Alastair G McEwen; Yves Nominé; Corinne Wendling; Pierre Poussin-Courmontagne; Laetitia Voilquin; Pascal Eberling; Frank Ruffenach; Jean Cavarelli; John Slee; Timothy P Levine; Guillaume Drin; Catherine Tomasetto; Fabien Alpy
Journal:  EMBO J       Date:  2020-10-30       Impact factor: 11.598

9.  Improved bacterial recombineering by parallelized protein discovery.

Authors:  Timothy M Wannier; Akos Nyerges; Helene M Kuchwara; Márton Czikkely; Dávid Balogh; Gabriel T Filsinger; Nathaniel C Borders; Christopher J Gregg; Marc J Lajoie; Xavier Rios; Csaba Pál; George M Church
Journal:  Proc Natl Acad Sci U S A       Date:  2020-05-28       Impact factor: 11.205

10.  A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection.

Authors:  Benjamin Schwessinger; Xiang Li; Thomas L Ellinghaus; Leanne Jade G Chan; Tong Wei; Anna Joe; Nicholas Thomas; Rory Pruitt; Paul D Adams; Maw Sheng Chern; Christopher J Petzold; Chang C Liu; Pamela C Ronald
Journal:  Integr Biol (Camb)       Date:  2015-11-27       Impact factor: 2.192
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