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Literature DB >> 16934461

Genetic introduction of a diketone-containing amino acid into proteins.

Huaqiang Zeng¹, Jianming Xie, Peter G Schultz.

Abstract

An orthogonal tRNA/aminoacyl-tRNA synthetase pair was evolved that makes possible the site-specific incorporation of an unnatural amino acid bearing a beta-diketone side chain into proteins in Escherichia coli with high translational efficiency and fidelity. Proteins containing this unnatural amino acid can be efficiently and selectively modified with hydroxylamine derivatives of fluorophores and other biophysical probes.

Entities: Chemical Species

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Year: 2006 PMID： 16934461 DOI： 10.1016/j.bmcl.2006.07.094

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

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Cited

10 in total

Review 1. Designer proteins: applications of genetic code expansion in cell biology.

Authors: Lloyd Davis; Jason W Chin
Journal: Nat Rev Mol Cell Biol Date: 2012-02-15 Impact factor: 94.444

2. A genetically encoded aldehyde for rapid protein labelling.

Authors: Alfred Tuley; Yan-Jiun Lee; Bo Wu; Zhiyong U Wang; Wenshe R Liu
Journal: Chem Commun (Camb) Date: 2014-07-18 Impact factor: 6.222

Review 3. Oximes and Hydrazones in Bioconjugation: Mechanism and Catalysis.

Authors: Dominik K Kölmel; Eric T Kool
Journal: Chem Rev Date: 2017-06-22 Impact factor: 60.622

4. A genetically encoded acrylamide functionality.

Authors: Yan-Jiun Lee; Bo Wu; Jeffrey E Raymond; Yu Zeng; Xinqiang Fang; Karen L Wooley; Wenshe R Liu
Journal: ACS Chem Biol Date: 2013-06-18 Impact factor: 5.100

Review 5. Synthesis at the interface of chemistry and biology.

Authors: Xu Wu; Peter G Schultz
Journal: J Am Chem Soc Date: 2009-09-09 Impact factor: 15.419

6. Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant.

Authors: Yane-Shih Wang; Xinqiang Fang; Hsueh-Ying Chen; Bo Wu; Zhiyong U Wang; Christian Hilty; Wenshe R Liu
Journal: ACS Chem Biol Date: 2012-11-19 Impact factor: 5.100

Genetic introduction of a diketone-containing amino acid into proteins.

Review 1. Designer proteins: applications of genetic code expansion in cell biology.

2. A genetically encoded aldehyde for rapid protein labelling.

Review 3. Oximes and Hydrazones in Bioconjugation: Mechanism and Catalysis.

4. A genetically encoded acrylamide functionality.

Review 5. Synthesis at the interface of chemistry and biology.

6. Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant.

7. Efficient Amber Suppression via Ribosomal Skipping for In Situ Synthesis of Photoconditional Nanobodies.

8. Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction.

Review 9. Designing logical codon reassignment - Expanding the chemistry in biology.

10. An overview of chemo- and site-selectivity aspects in the chemical conjugation of proteins.