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Literature DB >> 24608179

Single-molecule motions enable direct visualization of biomolecular interactions in solution.

Abstract

Biomolecular interactions are generally accompanied by modifications in size and charge of biomolecules at the nanometer scale. Here we describe a single-molecule method to sense these changes in real time based on statistical learning of diffusive and electric field-induced motion parameters of a trapped molecule in solution. We demonstrate the approach by resolving a monomer-trimer mixture along a protein dissociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule.

Mesh：

Substances：
Solutions
Solvents
DNA

Year: 2014 PMID： 24608179 DOI： 10.1038/nmeth.2882

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

20 in total

Review 1. Polymer wall coatings for capillary electrophoresis.

Authors: J Horvath; V Dolník
Journal: Electrophoresis Date: 2001 Impact factor: 3.535

2. Polyelectrolyte surface interface for single-molecule fluorescence studies of DNA polymerase.

Authors: Emil P Kartalov; Marc A Unger; Stephen R Quake
Journal: Biotechniques Date: 2003-03 Impact factor: 1.993

Review 3. Single-molecule approaches to characterizing kinetics of biomolecular interactions.

Authors: Antoine M van Oijen
Journal: Curr Opin Biotechnol Date: 2010-10-29 Impact factor: 9.740

4. Nonblinking and long-lasting single-molecule fluorescence imaging.

Authors: Ivan Rasnik; Sean A McKinney; Taekjip Ha
Journal: Nat Methods Date: 2006-10-01 Impact factor: 28.547

5. Subunit counting in membrane-bound proteins.

Authors: Maximilian H Ulbrich; Ehud Y Isacoff
Journal: Nat Methods Date: 2007-03-18 Impact factor: 28.547

6. Suppressing Brownian motion of individual biomolecules in solution.

Authors: Adam E Cohen; W E Moerner
Journal: Proc Natl Acad Sci U S A Date: 2006-03-14 Impact factor: 11.205

7. An Adaptive Anti-Brownian ELectrokinetic trap with real-time information on single-molecule diffusivity and mobility.

Authors: Quan Wang; W E Moerner
Journal: ACS Nano Date: 2011-06-03 Impact factor: 15.881

8. Electrokinetic trapping at the one nanometer limit.

Authors: Alexander P Fields; Adam E Cohen
Journal: Proc Natl Acad Sci U S A Date: 2011-05-11 Impact factor: 11.205

9. Continuous throughput and long-term observation of single-molecule FRET without immobilization.

Authors: Swati Tyagi; Virginia VanDelinder; Niccolò Banterle; Gustavo Fuertes; Sigrid Milles; Morgane Agez; Edward A Lemke
Journal: Nat Methods Date: 2014-01-19 Impact factor: 28.547

10. DINAMelt web server for nucleic acid melting prediction.

Authors: Nicholas R Markham; Michael Zuker
Journal: Nucleic Acids Res Date: 2005-07-01 Impact factor: 16.971

32 in total

1. Dissecting pigment architecture of individual photosynthetic antenna complexes in solution.

Authors: Quan Wang; W E Moerner
Journal: Proc Natl Acad Sci U S A Date: 2015-10-05 Impact factor: 11.205

2. Improving z-tracking accuracy in the two-photon single-particle tracking microscope.

Authors: C Liu; Y-L Liu; E P Perillo; N Jiang; A K Dunn; H-C Yeh
Journal: Appl Phys Lett Date: 2015-10-12 Impact factor: 3.791

3. Transition from stochastic events to deterministic ensemble average in electron transfer reactions revealed by single-molecule conductance measurement.

Authors: Yueqi Li; Hui Wang; Zixiao Wang; Yanjun Qiao; Jens Ulstrup; Hong-Yuan Chen; Gang Zhou; Nongjian Tao
Journal: Proc Natl Acad Sci U S A Date: 2019-02-08 Impact factor: 11.205

Single-molecule motions enable direct visualization of biomolecular interactions in solution.

Review 1. Polymer wall coatings for capillary electrophoresis.

2. Polyelectrolyte surface interface for single-molecule fluorescence studies of DNA polymerase.

Review 3. Single-molecule approaches to characterizing kinetics of biomolecular interactions.

4. Nonblinking and long-lasting single-molecule fluorescence imaging.

5. Subunit counting in membrane-bound proteins.

6. Suppressing Brownian motion of individual biomolecules in solution.

7. An Adaptive Anti-Brownian ELectrokinetic trap with real-time information on single-molecule diffusivity and mobility.

8. Electrokinetic trapping at the one nanometer limit.

9. Continuous throughput and long-term observation of single-molecule FRET without immobilization.

10. DINAMelt web server for nucleic acid melting prediction.

1. Dissecting pigment architecture of individual photosynthetic antenna complexes in solution.

2. Improving z-tracking accuracy in the two-photon single-particle tracking microscope.

3. Transition from stochastic events to deterministic ensemble average in electron transfer reactions revealed by single-molecule conductance measurement.

4. Unraveling the Thousand Word Picture: An Introduction to Super-Resolution Data Analysis.

Review 5. Feedback traps for virtual potentials.

6. Single-molecule electrometry.

7. 3D single-molecule tracking enables direct hybridization kinetics measurement in solution.

8. Single-molecule methods leap ahead.

9. Single-Molecule Tracking and Its Application in Biomolecular Binding Detection.

Review 10. Single-molecule spectroscopy and imaging over the decades.