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Literature DB >> 16537418

Suppressing Brownian motion of individual biomolecules in solution.

Abstract

Single biomolecules in free solution have long been of interest for detailed study by optical methods, but Brownian motion prevents the observation of one single molecule for extended periods. We have used an anti-Brownian electrokinetic (ABEL) trap to trap individual protein molecules in free solution, under ambient conditions, without requiring any attachment to beads or surfaces. We also demonstrate trapping and manipulation of single virus particles, lipid vesicles, and fluorescent semiconductor nanocrystals.

Entities: Chemical

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Year: 2006 PMID： 16537418 PMCID： PMC1450176 DOI： 10.1073/pnas.0509976103

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

12 in total

Suppressing Brownian motion of individual biomolecules in solution.

1. Tracking single proteins within cells.

2. Osmotic pressure inhibition of DNA ejection from phage.

3. Protein patterns at lipid bilayer junctions.

4. 3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells.

5. Microfabricated arrays of femtoliter chambers allow single molecule enzymology.

6. Single-molecule measurement of protein folding kinetics.

7. Observation of a single-beam gradient force optical trap for dielectric particles.

8. Optical trapping and manipulation of viruses and bacteria.

9. Orientation effects on the electrophoretic mobility of rod-shaped molecules in free solution.

10. Electrical manipulation of glycan-phosphatidyl inositol-tethered proteins in planar supported bilayers.

1. Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase.

2. A single-molecule view of chaperonin cooperativity.

3. Hydrodynamic trapping of molecules in lipid bilayers.

4. Optimal strategy for trapping single fluorescent molecules in solution using the ABEL trap.

5. Single-molecule spectroscopy: caught in a trap.

6. Geometry-induced electrostatic trapping of nanometric objects in a fluid.

7. Dissecting pigment architecture of individual photosynthetic antenna complexes in solution.

8. Temperature-independent porous nanocontainers for single-molecule fluorescence studies.

9. Utilizing the information content in two-state trajectories.

10. Single-Molecule Tracking and Its Application in Biomolecular Binding Detection.