Literature DB >> 17369835

Subunit counting in membrane-bound proteins.

Maximilian H Ulbrich1, Ehud Y Isacoff.   

Abstract

The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.

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Year:  2007        PMID： 17369835      PMCID： PMC2744285          DOI： 10.1038/nmeth1024

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  16 in total

1.  Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.

Authors:  Y He; M Ruiz; J W Karpen
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

2.  Rod cyclic nucleotide-gated channels have a stoichiometry of three CNGA1 subunits and one CNGB1 subunit.

Authors:  Jie Zheng; Matthew C Trudeau; William N Zagotta
Journal:  Neuron       Date:  2002-12-05       Impact factor: 17.173

3.  Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.

Authors:  E R Liman; J Tytgat; P Hess
Journal:  Neuron       Date:  1992-11       Impact factor: 17.173

4.  Stoichiometry of recombinant N-methyl-D-aspartate receptor channels inferred from single-channel current patterns.

Authors:  L S Premkumar; A Auerbach
Journal:  J Gen Physiol       Date:  1997-11       Impact factor: 4.086

5.  Stoichiometry and turnover in single, functioning membrane protein complexes.

Authors:  Mark C Leake; Jennifer H Chandler; George H Wadhams; Fan Bai; Richard M Berry; Judith P Armitage
Journal:  Nature       Date:  2006-09-13       Impact factor: 49.962

6.  Functional stoichiometry of Shaker potassium channel inactivation.

Authors:  R MacKinnon; R W Aldrich; A W Lee
Journal:  Science       Date:  1993-10-29       Impact factor: 47.728

7.  Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits.

Authors:  Jon E Chatterton; Marc Awobuluyi; Louis S Premkumar; Hiroto Takahashi; Maria Talantova; Yeonsook Shin; Jiankun Cui; Shichun Tu; Kevin A Sevarino; Nobuki Nakanishi; Gang Tong; Stuart A Lipton; Dongxian Zhang
Journal:  Nature       Date:  2002-01-30       Impact factor: 49.962

8.  The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry.

Authors:  Haining Zhong; Laurie L Molday; Robert S Molday; King-Wai Yau
Journal:  Nature       Date:  2002-11-14       Impact factor: 49.962

9.  Distinct structural determinants of efficacy and sensitivity in the ligand-binding domain of cyclic nucleotide-gated channels.

Authors:  Edgar C Young; Natalia Krougliak
Journal:  J Biol Chem       Date:  2003-10-31       Impact factor: 5.157

10.  Structural rearrangements in single ion channels detected optically in living cells.

Authors:  Alois Sonnleitner; Lidia M Mannuzzu; Susumu Terakawa; Ehud Y Isacoff
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-12       Impact factor: 11.205
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  314 in total

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Journal:  Nano Lett       Date:  2012-06-08       Impact factor: 11.189

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Journal:  J Mol Struct       Date:  2014-11-05       Impact factor: 3.196

Review 3.  Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging.

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Journal:  Annu Rev Phys Chem       Date:  2012-01-30       Impact factor: 12.703

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Authors:  Trevor G Smart; Pierre Paoletti
Journal:  Cold Spring Harb Perspect Biol       Date:  2012-03-01       Impact factor: 10.005

5.  Ligand-gated ion channels: from genes to behaviour.

Authors:  Derek Bowie
Journal:  J Physiol       Date:  2012-01-01       Impact factor: 5.182

6.  Quantification of fluorophore copy number from intrinsic fluctuations during fluorescence photobleaching.

Authors:  Chitra R Nayak; Andrew D Rutenberg
Journal:  Biophys J       Date:  2011-11-01       Impact factor: 4.033

7.  Single molecule fluorescence study of the Bacillus thuringiensis toxin Cry1Aa reveals tetramerization.

Authors:  Nicolas Groulx; Hugo McGuire; Raynald Laprade; Jean-Louis Schwartz; Rikard Blunck
Journal:  J Biol Chem       Date:  2011-10-17       Impact factor: 5.157

8.  Polyarginine induces an antitumor immune response through binding to toll-like receptor 4.

Authors:  Yong Yang; Joy Wolfram; Xiaohong Fang; Haifa Shen; Mauro Ferrari
Journal:  Small       Date:  2013-12-10       Impact factor: 13.281

9.  Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging.

Authors:  Roubina Tatavosian; Chao Yu Zhen; Huy Nguyen Duc; Maggie M Balas; Aaron M Johnson; Xiaojun Ren
Journal:  J Biol Chem       Date:  2015-09-17       Impact factor: 5.157

10.  Regulation of Kv2.1 K(+) conductance by cell surface channel density.

Authors:  Philip D Fox; Rob J Loftus; Michael M Tamkun
Journal:  J Neurosci       Date:  2013-01-16       Impact factor: 6.167
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