Literature DB >> 24441935

Continuous throughput and long-term observation of single-molecule FRET without immobilization.

Swati Tyagi1, Virginia VanDelinder2, Niccolò Banterle3, Gustavo Fuertes3, Sigrid Milles3, Morgane Agez3, Edward A Lemke3.   

Abstract

We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.

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Year:  2014        PMID： 24441935     DOI： 10.1038/nmeth.2809

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  26 in total

1.  Fluorescence correlation spectroscopy.

Authors:  Joachim D Müller; Yan Chen; Enrico Gratton
Journal:  Methods Enzymol       Date:  2003       Impact factor: 1.600

2.  Labeling of fusion proteins with synthetic fluorophores in live cells.

Authors:  Antje Keppler; Horst Pick; Claudio Arrivoli; Horst Vogel; Kai Johnsson
Journal:  Proc Natl Acad Sci U S A       Date:  2004-06-28       Impact factor: 11.205

Review 3.  Single-molecule approaches embrace molecular cohorts.

Authors:  Taekjip Ha
Journal:  Cell       Date:  2013-08-15       Impact factor: 41.582

Review 4.  A practical guide to single-molecule FRET.

Authors:  Rahul Roy; Sungchul Hohng; Taekjip Ha
Journal:  Nat Methods       Date:  2008-06       Impact factor: 28.547

5.  Single-molecule fluorescence experiments determine protein folding transition path times.

Authors:  Hoi Sung Chung; Kevin McHale; John M Louis; William A Eaton
Journal:  Science       Date:  2012-02-24       Impact factor: 47.728

Review 6.  What precision-protein-tuning and nano-resolved single molecule sciences can do for each other.

Authors:  Sigrid Milles; Edward A Lemke
Journal:  Bioessays       Date:  2012-11-13       Impact factor: 4.345

7.  Facilitated aggregation of FG nucleoporins under molecular crowding conditions.

Authors:  Sigrid Milles; Khanh Huy Bui; Christine Koehler; Mikhail Eltsov; Martin Beck; Edward A Lemke
Journal:  EMBO Rep       Date:  2012-12-14       Impact factor: 8.807

8.  Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153.

Authors:  Sigrid Milles; Edward A Lemke
Journal:  Biophys J       Date:  2011-10-05       Impact factor: 4.033

9.  Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe.

Authors:  Xinghua Shi; John Lim; Taekjip Ha
Journal:  Anal Chem       Date:  2010-07-15       Impact factor: 6.986

10.  Extracellular transglutaminase 2 is catalytically inactive, but is transiently activated upon tissue injury.

Authors:  Matthew Siegel; Pavel Strnad; R Edward Watts; Kihang Choi; Bana Jabri; M Bishr Omary; Chaitan Khosla
Journal:  PLoS One       Date:  2008-03-26       Impact factor: 3.240
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  18 in total

Review 1.  High-throughput single-molecule studies of protein-DNA interactions.

Authors:  Aaron D Robison; Ilya J Finkelstein
Journal:  FEBS Lett       Date:  2014-05-21       Impact factor: 4.124

2.  An in vitro tag-and-modify protein sample generation method for single-molecule fluorescence resonance energy transfer.

Authors:  Kambiz M Hamadani; Jesse Howe; Madeleine K Jensen; Peng Wu; Jamie H D Cate; Susan Marqusee
Journal:  J Biol Chem       Date:  2017-07-28       Impact factor: 5.157

3.  Single-molecule motions enable direct visualization of biomolecular interactions in solution.

Authors:  Quan Wang; W E Moerner
Journal:  Nat Methods       Date:  2014-03-09       Impact factor: 28.547

4.  Optimal Background Estimators in Single-Molecule FRET Microscopy.

Authors:  Søren Preus; Lasse L Hildebrandt; Victoria Birkedal
Journal:  Biophys J       Date:  2016-09-20       Impact factor: 4.033

5.  ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling.

Authors:  Hugh Wilson; Quan Wang
Journal:  Nat Methods       Date:  2021-06-14       Impact factor: 28.547

Review 6.  Single-molecule fluorescence resonance energy transfer in molecular biology.

Authors:  Dibyendu K Sasmal; Laura E Pulido; Shan Kasal; Jun Huang
Journal:  Nanoscale       Date:  2016-12-08       Impact factor: 7.790

Review 7.  Macromolecular transport between the nucleus and the cytoplasm: Advances in mechanism and emerging links to disease.

Authors:  Elizabeth J Tran; Megan C King; Anita H Corbett
Journal:  Biochim Biophys Acta       Date:  2014-08-09

Review 8.  Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.

Authors:  Alexander C DeHaven; Ian S Norden; Aaron A Hoskins
Journal:  Wiley Interdiscip Rev RNA       Date:  2016-05-20       Impact factor: 9.957

Review 9.  Deciphering Complexity in Molecular Biophysics with Single-Molecule Resolution.

Authors:  Ashok A Deniz
Journal:  J Mol Biol       Date:  2015-12-19       Impact factor: 5.469

10.  Measuring DNA Hybridization Kinetics in Live Cells Using a Time-Resolved 3D Single-Molecule Tracking Method.

Authors:  Yuan-I Chen; Yin-Jui Chang; Trung Duc Nguyen; Cong Liu; Stephanie Phillion; Yu-An Kuo; Huong T Vu; Angela Liu; Yen-Liang Liu; Soonwoo Hong; Pengyu Ren; Thomas E Yankeelov; Hsin-Chih Yeh
Journal:  J Am Chem Soc       Date:  2019-09-26       Impact factor: 15.419
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