| Literature DB >> 31509386 |
Yuan-I Chen1, Yin-Jui Chang2, Trung Duc Nguyen1, Cong Liu1, Stephanie Phillion1, Yu-An Kuo1, Huong T Vu1, Angela Liu1, Yen-Liang Liu1, Soonwoo Hong1, Pengyu Ren1, Thomas E Yankeelov1,3,4,5,6, Hsin-Chih Yeh1,7.
Abstract
Single-molecule detection enables direct characterization of annealing/melting kinetics of nucleic acids without the need for synchronization of molecular states, but the current experiments are not carried out in a native cellular context. Here we describe an integrated 3D single-molecule tracking and lifetime measurement method that can follow individual DNA molecules diffusing inside a mammalian cell and observe multiple annealing and melting events on the same molecules. By comparing the hybridization kinetics of the same DNA strand in vitro, we found the association constants can be 13- to 163-fold higher in the molecular crowding cellular environment.Entities:
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Year: 2019 PMID： 31509386 PMCID： PMC7179573 DOI： 10.1021/jacs.9b08036
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419