Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Single-round, multiplexed antibody mimetic design through mRNA display.

Literature DB >> 23125174

Single-round, multiplexed antibody mimetic design through mRNA display.

C Anders Olson¹, Jeff Nie, Jonathan Diep, Ibrahim Al-Shyoukh, Terry T Takahashi, Laith Q Al-Mawsawi, Jennifer M Bolin, Angela L Elwell, Scott Swanson, Ron Stewart, James A Thomson, H Tom Soh, Richard W Roberts, Ren Sun.

Abstract

In a single round: By combining the high-efficiency enrichment through the continuous-flow magnetic separation (CFMS) technique with the analytical power of next-generation sequencing, the generation of antibody mimetics with a single round of mRNA display is made possible. This approach eliminates iterative selection cycles and provides a path to fully automated ligand generation (see picture).

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 2012 PMID： 23125174 PMCID： PMC3832244 DOI： 10.1002/anie.201207005

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

23 in total

1. Convergent solutions to binding at a protein-protein interface.

Authors: W L DeLano; M H Ultsch; A M de Vos; J A Wells
Journal: Science Date: 2000-02-18 Impact factor: 47.728

2. Comparative study of IgG binding to proteins G and A: nonequilibrium kinetic and binding constant determination with the acoustic waveguide device.

Authors: K Saha; F Bender; E Gizeli
Journal: Anal Chem Date: 2003-02-15 Impact factor: 6.986

3. Accelerated screening of phage-display output with alkaline phosphatase fusions.

Authors: Zhaozhong Han; Ece Karatan; Michael D Scholle; John McCafferty; Brian K Kay
Journal: Comb Chem High Throughput Screen Date: 2004-02 Impact factor: 1.339

4. Aptamer selection by high-throughput sequencing and informatic analysis.

Authors: Shawn Hoon; Bin Zhou; Kim D Janda; Sydney Brenner; Jonathan Scolnick
Journal: Biotechniques Date: 2011-12 Impact factor: 1.993

5. In vitro selection of protein and peptide libraries using mRNA display.

Authors: Terry T Takahashi; Richard W Roberts
Journal: Methods Mol Biol Date: 2009

Review 6. FN3: a new protein scaffold reaches the clinic.

Authors: Laird Bloom; Valerie Calabro
Journal: Drug Discov Today Date: 2009-07-02 Impact factor: 7.851

7. mRNA display selection of a high-affinity, modification-specific phospho-IkappaBalpha-binding fibronectin.

Authors: C Anders Olson; Hsiang-I Liao; Ren Sun; Richard W Roberts
Journal: ACS Chem Biol Date: 2008-07-01 Impact factor: 5.100

8. RNA-peptide fusions for the in vitro selection of peptides and proteins.

Authors: R W Roberts; J W Szostak
Journal: Proc Natl Acad Sci U S A Date: 1997-11-11 Impact factor: 11.205

9. Construction, bacterial expression, and characterization of hapten-specific single-chain Fv and alkaline phosphatase fusion protein.

Authors: C Suzuki; H Ueda; E Suzuki; T Nagamune
Journal: J Biochem Date: 1997-08 Impact factor: 3.387

10. Robust, quantitative analysis of proteins using peptide immunoreagents, in vitro translation, and an ultrasensitive acoustic resonant sensor.

Authors: Farzad Jalali-Yazdi; Jasmine M Corbin; Terry T Takahashi; Richard W Roberts
Journal: Anal Chem Date: 2014-05-02 Impact factor: 6.986

Single-round, multiplexed antibody mimetic design through mRNA display.

1. Convergent solutions to binding at a protein-protein interface.

2. Comparative study of IgG binding to proteins G and A: nonequilibrium kinetic and binding constant determination with the acoustic waveguide device.

3. Accelerated screening of phage-display output with alkaline phosphatase fusions.

4. Aptamer selection by high-throughput sequencing and informatic analysis.

5. In vitro selection of protein and peptide libraries using mRNA display.

Review 6. FN3: a new protein scaffold reaches the clinic.

7. mRNA display selection of a high-affinity, modification-specific phospho-IkappaBalpha-binding fibronectin.

8. RNA-peptide fusions for the in vitro selection of peptides and proteins.

9. Construction, bacterial expression, and characterization of hapten-specific single-chain Fv and alkaline phosphatase fusion protein.

10. Acyclic identification of aptamers for human alpha-thrombin using over-represented libraries and deep sequencing.

1. High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing.

2. Identification of target-binding peptide motifs by high-throughput sequencing of phage-selected peptides.

3. A comprehensive biophysical description of pairwise epistasis throughout an entire protein domain.

Review 4. Peptide aptamers: development and applications.

5. RasIns: Genetically Encoded Intrabodies of Activated Ras Proteins.

Review 6. Conceptual and methodological advances in cell-free directed evolution.

Review 7. Advances in the directed evolution of proteins.

Review 8. Directing evolution of novel ligands by mRNA display.

9. Reverse Engineering of Vaccine Antigens Using High Throughput Sequencing-enhanced mRNA Display.

10. Robust, quantitative analysis of proteins using peptide immunoreagents, in vitro translation, and an ultrasensitive acoustic resonant sensor.