| Literature DB >> 23084833 |
Mary T Couvillion1, Gergana Bounova, Elizabeth Purdom, Terence P Speed, Kathleen Collins.
Abstract
Emerging evidence suggests that Argonaute (Ago)/Piwi proteins have diverse functions in the nucleus and cytoplasm, but the molecular mechanisms employed in the nucleus remain poorly defined. The Tetrahymena thermophila Ago/Piwi protein Twi12 is essential for growth and functions in the nucleus. Twi12-bound small RNAs (sRNAs) are 3' tRNA fragments that contain modified bases and thus are attenuated for base pairing to targets. We show that Twi12 assembles an unexpected complex with the nuclear exonuclease Xrn2. Twi12 functions to stabilize and localize Xrn2, as well as to stimulate its exonuclease activity. Twi12 function depends on sRNA binding, which is required for its nuclear import. Depletion of Twi12 or Xrn2 induces a cellular ribosomal RNA processing defect known to result from limiting Xrn2 activity in other organisms. Our findings suggest a role for an Ago/Piwi protein and 3' tRNA fragments in nuclear RNA metabolism.Entities:
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Year: 2012 PMID： 23084833 PMCID： PMC3513674 DOI： 10.1016/j.molcel.2012.09.010
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970