Literature DB >> 22952270

Comparison of BD GeneOhm Cdiff and Seegene Seeplex ACE PCR assays using toxigenic Clostridium difficile culture for direct detection of tcdB from stool specimens.

Bo-Moon Shin1, Se Jin Mun, Soo Jin Yoo, Eun Young Kuak.   

Abstract

We evaluated the performances of 2 PCR assays (BD GeneOhm and Seegene ACE) for direct detection of tcdB from stool specimens. The concordance rate between BD and Seegene was 96.3%. The sensitivities, specificities, positive predictive values (PPVs), and negative predictive values (NPVs) of BD and Seegene were 95.7%, 96.5%, 91.8%, and 98.2% and 90.0%, 97.1%, 92.6%, and 96.0%, respectively.

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Year:  2012        PMID： 22952270      PMCID： PMC3486254          DOI： 10.1128/JCM.01440-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Detection of toxigenic Clostridium difficile: comparison of the cell culture neutralization, Xpert C. difficile, Xpert C. difficile/Epi, and Illumigene C. difficile assays.

Authors:  P Pancholi; C Kelly; M Raczkowski; J M Balada-Llasat
Journal:  J Clin Microbiol       Date:  2012-01-25       Impact factor: 5.948

2.  Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients.

Authors:  Renate J van den Berg; Lesla S Bruijnesteijn van Coppenraet; Hendrik-Jan Gerritsen; Hubert P Endtz; Eric R van der Vorm; Ed J Kuijper
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

3.  Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile.

Authors:  Ludovic Lemee; Anne Dhalluin; Sabrina Testelin; Marie-Andre Mattrat; Karine Maillard; Jean-François Lemeland; Jean-Louis Pons
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

4.  Evaluation of the Cepheid Xpert Clostridium difficile Epi assay for diagnosis of Clostridium difficile infection and typing of the NAP1 strain at a cancer hospital.

Authors:  N Esther Babady; Jeffrey Stiles; Phyllis Ruggiero; Perminder Khosa; David Huang; Susan Shuptar; Mini Kamboj; Timothy E Kiehn
Journal:  J Clin Microbiol       Date:  2010-10-13       Impact factor: 5.948

5.  Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples.

Authors:  Paul D Stamper; Romina Alcabasa; Deborah Aird; Wisal Babiker; Jennifer Wehrlin; Ijeoma Ikpeama; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

6.  Algorithm combining toxin immunoassay and stool culture for diagnosis of Clostridium difficile infection.

Authors:  Bo-Moon Shin; Eun Young Kuak; Eun Joo Lee; J Glenn Songer
Journal:  J Clin Microbiol       Date:  2009-07-22       Impact factor: 5.948

7.  Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile.

Authors:  Megan E Reller; Clara A Lema; Trish M Perl; Mian Cai; Tracy L Ross; Kathleen A Speck; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2007-09-05       Impact factor: 5.948

8.  Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection.

Authors:  Elizabeth J Kvach; David Ferguson; Paul F Riska; Marie L Landry
Journal:  J Clin Microbiol       Date:  2009-10-28       Impact factor: 5.948

9.  Evaluation of a new commercial TaqMan PCR assay for direct detection of the clostridium difficile toxin B gene in clinical stool specimens.

Authors:  Paul D Stamper; Wisal Babiker; Romina Alcabasa; Deborah Aird; Jennifer Wehrlin; Ijeoma Ikpeama; Linda Gluck; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2009-10-21       Impact factor: 5.948

10.  Comparison of a rapid molecular method, the BD GeneOhm Cdiff assay, to the most frequently used laboratory tests for detection of toxin-producing Clostridium difficile in diarrheal feces.

Authors:  Gabriella Terhes; Edit Urbán; József Sóki; Eniko Nacsa; Elisabeth Nagy
Journal:  J Clin Microbiol       Date:  2009-09-30       Impact factor: 5.948
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  6 in total

1.  Evaluation of a new automated homogeneous PCR assay, GenomEra C. difficile, for rapid detection of Toxigenic Clostridium difficile in fecal specimens.

Authors:  Jari J Hirvonen; Silja Mentula; Suvi-Sirkku Kaukoranta
Journal:  J Clin Microbiol       Date:  2013-06-26       Impact factor: 5.948

2.  Comparison of BD Max Cdiff and GenomEra C. difficile molecular assays for detection of toxigenic Clostridium difficile from stools in conventional sample containers and in FecalSwabs.

Authors:  J J Hirvonen; S-S Kaukoranta
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2015-01-24       Impact factor: 3.267

Review 3.  Current knowledge on the laboratory diagnosis of Clostridium difficile infection.

Authors:  Adrián Martínez-Meléndez; Adrián Camacho-Ortiz; Rayo Morfin-Otero; Héctor Jesús Maldonado-Garza; Licet Villarreal-Treviño; Elvira Garza-González
Journal:  World J Gastroenterol       Date:  2017-03-07       Impact factor: 5.742

4.  Laboratory Diagnostic Methods for Clostridioides difficile Infection: the First Systematic Review and Meta-analysis in Korea.

Authors:  Hae-Sun Chung; Jeong Su Park; Bo-Moon Shin
Journal:  Ann Lab Med       Date:  2021-03-01       Impact factor: 3.464

5.  Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.

Authors:  Bo-Moon Shin; Eun Joo Lee
Journal:  Ann Lab Med       Date:  2013-12-06       Impact factor: 3.464

6.  Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens.

Authors:  Bo-Moon Shin; Sun Mee Yoo; Won Chang Shin
Journal:  Ann Lab Med       Date:  2016-03       Impact factor: 3.464
  6 in total

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