PURPOSE: The use prosthetic materials for the surgical repair of abdominal wall defects has become almost standard practice. This study was designed to assess the expression of different growth factors (VEGF/TGF-β1) and macrophages during the early host tissue incorporation of several polypropylene lightweight (PP-LW)-including one partially absorbable-and heavyweight (PP-HW) prosthetic meshes. METHODS: Ventral defects were created in the anterior abdominal wall of New Zealand rabbits and repaired by fixing PP-LW meshes of different pore size and a low porosity PP-HW mesh to the edges of the defect. Following killing 14 days after implant, specimens were taken to examine TGF-β1/VEGF gene and protein expression by qRT-PCR and immunohistochemistry. The macrophage response was also assessed. RESULTS: All the materials showed good host tissue incorporation, with a more severe inflammatory reaction and greater numbers of macrophages recorded in the partially absorbable LW implants. Relative amounts of VEGF mRNA were significantly lower for the LW partially absorbable implants compared with the remaining LW meshes. Protein expression of VEGF showed undetectable or minimum staining in the different groups. TGF-β1 mRNA levels were also lower in the partially absorbable group compared with one of PP-LW type of mesh. Gene expression patterns were consistent with the TGF-β1 protein levels detected. CONCLUSIONS: The results suggest that VEGF and TGF-β1 expression were independent of mesh pore size. The expression of both growth factors and the macrophage response were correlated with the presence of biodegradable material in the mesh. The presence of absorbable material in the LW mesh gave rise to a more intense inflammatory reaction and the reduced synthesis of growth factors known to contribute to neotissue maturation.
PURPOSE: The use prosthetic materials for the surgical repair of abdominal wall defects has become almost standard practice. This study was designed to assess the expression of different growth factors (VEGF/TGF-β1) and macrophages during the early host tissue incorporation of several polypropylene lightweight (PP-LW)-including one partially absorbable-and heavyweight (PP-HW) prosthetic meshes. METHODS: Ventral defects were created in the anterior abdominal wall of New Zealand rabbits and repaired by fixing PP-LW meshes of different pore size and a low porosity PP-HW mesh to the edges of the defect. Following killing 14 days after implant, specimens were taken to examine TGF-β1/VEGF gene and protein expression by qRT-PCR and immunohistochemistry. The macrophage response was also assessed. RESULTS: All the materials showed good host tissue incorporation, with a more severe inflammatory reaction and greater numbers of macrophages recorded in the partially absorbable LW implants. Relative amounts of VEGF mRNA were significantly lower for the LW partially absorbable implants compared with the remaining LW meshes. Protein expression of VEGF showed undetectable or minimum staining in the different groups. TGF-β1 mRNA levels were also lower in the partially absorbable group compared with one of PP-LW type of mesh. Gene expression patterns were consistent with the TGF-β1 protein levels detected. CONCLUSIONS: The results suggest that VEGF and TGF-β1 expression were independent of mesh pore size. The expression of both growth factors and the macrophage response were correlated with the presence of biodegradable material in the mesh. The presence of absorbable material in the LW mesh gave rise to a more intense inflammatory reaction and the reduced synthesis of growth factors known to contribute to neotissue maturation.
Authors: G Di Vita; S Milano; M Frazzetta; R Patti; V Palazzolo; C Barbera; V Ferlazzo; P Leo; E Cillari Journal: Am J Surg Date: 2000-09 Impact factor: 2.565
Authors: Jaime A Cavallo; Andres A Roma; Mateusz S Jasielec; Jenny Ousley; Jennifer Creamer; Matthew D Pichert; Sara Baalman; Margaret M Frisella; Brent D Matthews; Corey R Deeken Journal: Surg Endosc Date: 2014-01-18 Impact factor: 4.584
Authors: C G Pereira-lucena; R Artigiani Neto; D T de Rezende; G de J Lopes-Filho; D Matos; M M Linhares Journal: Hernia Date: 2013-12-27 Impact factor: 4.739
Authors: Giulia Gigliobianco; Sabiniano Roman Regueros; Nadir I Osman; Julio Bissoli; Anthony J Bullock; Chris R Chapple; Sheila MacNeil Journal: Biomed Res Int Date: 2015-04-21 Impact factor: 3.411
Authors: Rosalia Patti; Anna Maria Caruso; Paolo Aiello; Giuseppe Livio Angelo; Salvatore Buscemi; Gaetano Di Vita Journal: BMC Surg Date: 2014-11-15 Impact factor: 2.102
Authors: Carlos Alberto Lima Utrabo; Nicolau Gregori Czeczko; Cesar Roberto Busato; Mário Rodrigues Montemór-Netto; Leandro Lipinski; Osvaldo Malafaia Journal: Arq Bras Cir Dig Date: 2021-06-11
Authors: Marcello Donati; Giovanna Brancato; Giuseppe Grosso; Giovanni Li Volti; Giuseppina La Camera; Francesco Cardì; Francesco Basile; Angelo Donati Journal: Medicine (Baltimore) Date: 2016-06 Impact factor: 1.889