Literature DB >> 22740125

A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.

Carole Gardiennet1, Anne K Schütz, Andreas Hunkeler, Britta Kunert, Laurent Terradot, Anja Böckmann, Beat H Meier.   

Abstract

Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 22740125     DOI： 10.1002/anie.201200779

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  39 in total

1.  Practical considerations over spectral quality in solid state NMR spectroscopy of soluble proteins.

Authors:  Marco Fragai; Claudio Luchinat; Giacomo Parigi; Enrico Ravera
Journal:  J Biomol NMR       Date:  2013-08-30       Impact factor: 2.835

2.  SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.

Authors:  Lucio Ferella; Claudio Luchinat; Enrico Ravera; Antonio Rosato
Journal:  J Biomol NMR       Date:  2013-11-17       Impact factor: 2.835

3.  Topical Developments in High-Field Dynamic Nuclear Polarization.

Authors:  Vladimir K Michaelis; Ta-Chung Ong; Matthew K Kiesewetter; Derik K Frantz; Joseph J Walish; Enrico Ravera; Claudio Luchinat; Timothy M Swager; Robert G Griffin
Journal:  Isr J Chem       Date:  2014-02-13       Impact factor: 3.333

4.  On the use of ultracentrifugal devices for sedimented solute NMR.

Authors:  Ivano Bertini; Frank Engelke; Leonardo Gonnelli; Benno Knott; Claudio Luchinat; David Osen; Enrico Ravera
Journal:  J Biomol NMR       Date:  2012-08-08       Impact factor: 2.835

5.  Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology.

Authors:  Ioannis Gelis; Veronika Vitzthum; Neha Dhimole; Marc A Caporini; Andreas Schedlbauer; Diego Carnevale; Sean R Connell; Paola Fucini; Geoffrey Bodenhausen
Journal:  J Biomol NMR       Date:  2013-05-21       Impact factor: 2.835

6.  Dynamics in the solid-state: perspectives for the investigation of amyloid aggregates, membrane proteins and soluble protein complexes.

Authors:  Rasmus Linser; Riddhiman Sarkar; Alexey Krushelnitzky; Andi Mainz; Bernd Reif
Journal:  J Biomol NMR       Date:  2014-03-05       Impact factor: 2.835

7.  Insights into the structure and dynamics of measles virus nucleocapsids by 1H-detected solid-state NMR.

Authors:  Emeline Barbet-Massin; Michele Felletti; Robert Schneider; Stefan Jehle; Guillaume Communie; Nicolas Martinez; Malene Ringkjøbing Jensen; Rob W H Ruigrok; Lyndon Emsley; Anne Lesage; Martin Blackledge; Guido Pintacuda
Journal:  Biophys J       Date:  2014-08-19       Impact factor: 4.033

8.  Segmental isotope labelling and solid-state NMR of a 12 × 59 kDa motor protein: identification of structural variability.

Authors:  Thomas Wiegand; Riccardo Cadalbert; Christine von Schroetter; Frédéric H-T Allain; Beat H Meier
Journal:  J Biomol NMR       Date:  2018-06-12       Impact factor: 2.835

9.  Gd3+-chelated lipid accelerates solid-state NMR spectroscopy of seven-transmembrane proteins.

Authors:  Chang Liu; Jing Liu; Xiaojun Xu; ShengQi Xiang; Shenlin Wang
Journal:  J Biomol NMR       Date:  2017-05-30       Impact factor: 2.835

10.  Protein-nucleotide contacts in motor proteins detected by DNP-enhanced solid-state NMR.

Authors:  Thomas Wiegand; Wei-Chih Liao; Ta Chung Ong; Alexander Däpp; Riccardo Cadalbert; Christophe Copéret; Anja Böckmann; Beat H Meier
Journal:  J Biomol NMR       Date:  2017-11-08       Impact factor: 2.835
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