Literature DB >> 21976756

Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC.

Søren Persson1, Joan N Jensen, Katharina E P Olsen.   

Abstract

A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA, tcdB, ctdA, and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC. The method has high specificity for PCR ribotype 027 and may identify other C. difficile strains of clinical and epidemiological importance.

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Year:  2011        PMID： 21976756      PMCID： PMC3232998          DOI： 10.1128/JCM.05161-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Outbreak of Clostridium difficile 027 in North Zealand, Denmark, 2008-2009.

Authors:  S Bacci; G St-Martin; B Olesen; B Bruun; K E P Olsen; E Møller Nielsen; K Mølbak
Journal:  Euro Surveill       Date:  2009-04-23

2.  Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

Authors:  P Bidet; V Lalande; B Salauze; B Burghoffer; V Avesani; M Delmée; A Rossier; F Barbut; J C Petit
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

3.  Clinical features of Clostridium difficile infection and molecular characterization of the isolated strains in a cohort of Danish hospitalized patients.

Authors:  L M Søes; I Brock; S Persson; J Simonsen; K E Pribil Olsen; M Kemp
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-07-10       Impact factor: 3.267

4.  Clinical features of Clostridium difficile-associated infections and molecular characterization of strains: results of a retrospective study, 2000-2004.

Authors:  Frédéric Barbut; Béatrice Gariazzo; Laetitia Bonné; Valérie Lalande; Béatrice Burghoffer; Ralucca Luiuz; Jean-Claude Petit
Journal:  Infect Control Hosp Epidemiol       Date:  2007-01-24       Impact factor: 3.254

5.  New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to a Danish strain collection.

Authors:  S Persson; M Torpdahl; K E P Olsen
Journal:  Clin Microbiol Infect       Date:  2008-11       Impact factor: 8.067

6.  Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection.

Authors:  Lynne M Sloan; Brian J Duresko; Daniel R Gustafson; Jon E Rosenblatt
Journal:  J Clin Microbiol       Date:  2008-04-23       Impact factor: 5.948

7.  Genetic relatedness of Clostridium difficile isolates from various origins determined by triple-locus sequence analysis based on toxin regulatory genes tcdC, tcdR, and cdtR.

Authors:  Philippe J M Bouvet; Michel R Popoff
Journal:  J Clin Microbiol       Date:  2008-10-01       Impact factor: 5.948

8.  Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans.

Authors:  Sylvia B Debast; Leo A M G van Leengoed; Abraham Goorhuis; Celine Harmanus; Ed J Kuijper; Aldert A Bergwerff
Journal:  Environ Microbiol       Date:  2009-02       Impact factor: 5.491

9.  Clostridium difficile toxin expression is inhibited by the novel regulator TcdC.

Authors:  Susana Matamouros; Patrick England; Bruno Dupuy
Journal:  Mol Microbiol       Date:  2007-06       Impact factor: 3.501

10.  Truncation in the tcdC region of the Clostridium difficile PathLoc of clinical isolates does not predict increased biological activity of Toxin B or Toxin A.

Authors:  Ruth Murray; Dave Boyd; Paul N Levett; Michael R Mulvey; Michelle J Alfa
Journal:  BMC Infect Dis       Date:  2009-06-28       Impact factor: 3.090
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  25 in total

1.  Diagnosis of Clostridium difficile: real-time PCR detection of toxin genes in faecal samples is more sensitive compared to toxigenic culture.

Authors:  M B F Jensen; K E P Olsen; X C Nielsen; A M Hoegh; R B Dessau; T Atlung; J Engberg
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-11-25       Impact factor: 3.267

2.  Clostridium difficile infections in Finland, 2008-2015: trends, diagnostics and ribotypes.

Authors:  S Mentula; S M Kotila; O Lyytikäinen; S Ibrahem; J Ollgren; A Virolainen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-05-30       Impact factor: 3.267

3.  U.S.-Based National Surveillance for Fidaxomicin Susceptibility of Clostridioides difficile-Associated Diarrheal Isolates from 2013 to 2016.

Authors:  C M Thorpe; L A McDermott; M K Tran; J Chang; S G Jenkins; E J C Goldstein; R Patel; B A Forbes; S Johnson; D N Gerding; D R Snydman
Journal:  Antimicrob Agents Chemother       Date:  2019-06-24       Impact factor: 5.191

4.  Premarket evaluations of the IMDx C. difficile for Abbott m2000 Assay and the BD Max Cdiff Assay.

Authors:  K A Stellrecht; A A Espino; V P Maceira; S M Nattanmai; S A Butt; D Wroblewski; G E Hannett; K A Musser
Journal:  J Clin Microbiol       Date:  2014-02-19       Impact factor: 5.948

5.  Investigation of toxin gene diversity and antimicrobial resistance of Clostridium difficile strains.

Authors:  Shanshan Zhu; Huaping Zhang; Xinsheng Zhang; Chao Wang; Guangming Fan; Weifeng Zhang; Gang Sun; Huihong Chen; Liming Zhang; Zhaoyun Li
Journal:  Biomed Rep       Date:  2014-07-08

6.  U.S.-Based National Sentinel Surveillance Study for the Epidemiology of Clostridium difficile-Associated Diarrheal Isolates and Their Susceptibility to Fidaxomicin.

Authors:  D R Snydman; L A McDermott; N V Jacobus; C Thorpe; S Stone; S G Jenkins; E J C Goldstein; R Patel; B A Forbes; S Mirrett; S Johnson; D N Gerding
Journal:  Antimicrob Agents Chemother       Date:  2015-08-03       Impact factor: 5.191

7.  Molecular characterization of toxigenic Clostridium difficile in a northern Italian hospital.

Authors:  Francesco G De Rosa; Paolo Cavallerio; Silvia Corcione; Caterina Parlato; Lucina Fossati; Roberto Serra; Giovanni Di Perri; Rossana Cavallo
Journal:  Curr Microbiol       Date:  2014-09-23       Impact factor: 2.188

8.  Simultaneous detection and characterization of toxigenic Clostridium difficile directly from clinical stool specimens.

Authors:  Hanjiang Lai; Chen Huang; Jian Cai; Julian Ye; Jun She; Yi Zheng; Liqian Wang; Yelin Wei; Weijia Fang; Xianjun Wang; Yi-Wei Tang; Yun Luo; Dazhi Jin
Journal:  Front Med       Date:  2017-10-17       Impact factor: 4.592

9.  Antimicrobial susceptibility and ribotypes of Clostridium difficile isolates from a Phase 2 clinical trial of ridinilazole (SMT19969) and vancomycin.

Authors:  David R Snydman; Laura A McDermott; Cheleste M Thorpe; Justin Chang; Jenna Wick; Seth T Walk; Richard J Vickers
Journal:  J Antimicrob Chemother       Date:  2018-08-01       Impact factor: 5.790

10.  Global Landscape of Clostridioides Difficile Phylogeography, Antibiotic Susceptibility, and Toxin Polymorphisms by Post-Hoc Whole-Genome Sequencing from the MODIFY I/II Studies.

Authors:  Hailong Zhao; David C Nickle; Zhen Zeng; Pierra Y T Law; Mark H Wilcox; Lan Chen; Ye Peng; Jie Meng; Ziqing Deng; Andrew Albright; Huanzi Zhong; Xun Xu; Shida Zhu; Judong Shen; Rebecca L Blanchard; Mary Beth Dorr; Peter M Shaw; Junhua Li
Journal:  Infect Dis Ther       Date:  2021-03-22
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