Literature DB >> 20729840

Rapid selection of transgenic C. elegans using antibiotic resistance.

Jennifer I Semple1, Rosa Garcia-Verdugo, Ben Lehner.   

Abstract

Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.

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Year:  2010        PMID： 20729840     DOI： 10.1038/nmeth.1495

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  12 in total

1.  Ballistic transformation of Caenorhabditis elegans.

Authors:  T Wilm; P Demel; H U Koop; H Schnabel; R Schnabel
Journal:  Gene       Date:  1999-03-18       Impact factor: 3.688

2.  Mutant Caenorhabditis elegans RNA polymerase II with a 20,000-fold reduced sensitivity to alpha-amanitin.

Authors:  T M Rogalski; M Golomb; D L Riddle
Journal:  Genetics       Date:  1990-12       Impact factor: 4.562

Review 3.  Evolution of development in nematodes related to C. elegans.

Authors:  Ralf J Sommer
Journal:  WormBook       Date:  2005-12-14

4.  Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.

Authors:  J P Morgenstern; H Land
Journal:  Nucleic Acids Res       Date:  1990-06-25       Impact factor: 16.971

5.  Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.

Authors:  V Praitis; E Casey; D Collar; J Austin
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

Review 6.  DNA transformation.

Authors:  C Mello; A Fire
Journal:  Methods Cell Biol       Date:  1995       Impact factor: 1.441

Review 7.  Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system.

Authors:  Rock Pulak
Journal:  Methods Mol Biol       Date:  2006

8.  Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

Authors:  C C Mello; J M Kramer; D Stinchcomb; V Ambros
Journal:  EMBO J       Date:  1991-12       Impact factor: 11.598

9.  Single-copy insertion of transgenes in Caenorhabditis elegans.

Authors:  Christian Frøkjaer-Jensen; M Wayne Davis; Christopher E Hopkins; Blake J Newman; Jason M Thummel; Søren-Peter Olesen; Morten Grunnet; Erik M Jorgensen
Journal:  Nat Genet       Date:  2008-10-26       Impact factor: 38.330

10.  The DAF-3 Smad binds DNA and represses gene expression in the Caenorhabditis elegans pharynx.

Authors:  J D Thatcher; C Haun; P G Okkema
Journal:  Development       Date:  1999-01       Impact factor: 6.868
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  41 in total

Review 1.  Modeling human neurodegenerative diseases in transgenic systems.

Authors:  Miguel A Gama Sosa; Rita De Gasperi; Gregory A Elder
Journal:  Hum Genet       Date:  2011-12-14       Impact factor: 4.132

2.  pTransgenesis: a cross-species, modular transgenesis resource.

Authors:  Nick R Love; Raphael Thuret; Yaoyao Chen; Shoko Ishibashi; Nitin Sabherwal; Roberto Paredes; Juliana Alves-Silva; Karel Dorey; Anna M Noble; Matthew J Guille; Yoshiki Sasai; Nancy Papalopulu; Enrique Amaya
Journal:  Development       Date:  2011-12       Impact factor: 6.868

3.  Generating transgenic nematodes by bombardment and antibiotic selection.

Authors:  Jennifer I Semple; Laura Biondini; Ben Lehner
Journal:  Nat Methods       Date:  2012-01-30       Impact factor: 28.547

Review 4.  The early bird catches the worm: new technologies for the Caenorhabditis elegans toolkit.

Authors:  Xiao Xu; Stuart K Kim
Journal:  Nat Rev Genet       Date:  2011-10-04       Impact factor: 53.242

Review 5.  From genes to function: the C. elegans genetic toolbox.

Authors:  Thomas Boulin; Oliver Hobert
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2011-11-28       Impact factor: 5.814

Review 6.  From "the Worm" to "the Worms" and Back Again: The Evolutionary Developmental Biology of Nematodes.

Authors:  Eric S Haag; David H A Fitch; Marie Delattre
Journal:  Genetics       Date:  2018-10       Impact factor: 4.562

Review 7.  Forward and reverse mutagenesis in C. elegans.

Authors:  Lena M Kutscher; Shai Shaham
Journal:  WormBook       Date:  2014-01-17

8.  High-throughput optical quantification of mechanosensory habituation reveals neurons encoding memory in Caenorhabditis elegans.

Authors:  Takuma Sugi; Yasuko Ohtani; Yuta Kumiya; Ryuji Igarashi; Masahiro Shirakawa
Journal:  Proc Natl Acad Sci U S A       Date:  2014-11-17       Impact factor: 11.205

9.  Puromycin- and methotrexate-resistance cassettes and optimized Cre-recombinase expression plasmids for use in yeast.

Authors:  Chris MacDonald; Robert C Piper
Journal:  Yeast       Date:  2015-03-19       Impact factor: 3.239

Review 10.  Genetic and epigenetic effects on centromere establishment.

Authors:  Yick Hin Ling; Zhongyang Lin; Karen Wing Yee Yuen
Journal:  Chromosoma       Date:  2019-11-28       Impact factor: 4.316
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