Literature DB >> 20637662

1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins.

Eugene C Lin1, Chin H Wu, Yuan Yang, Christopher V Grant, Stanley J Opella.   

Abstract

By incorporating homonuclear decoupling on both the (1)H and (13)C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with (13)C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of (15)N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described. (c) 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20637662      PMCID： PMC2926282          DOI： 10.1016/j.jmr.2010.06.011

Source DB:  PubMed          Journal:  J Magn Reson        ISSN： 1090-7807            Impact factor:   2.229


  16 in total

1.  THE REPLICATION OF DNA IN ESCHERICHIA COLI.

Authors:  M Meselson; F W Stahl
Journal:  Proc Natl Acad Sci U S A       Date:  1958-07-15       Impact factor: 11.205

2.  Selective averaging for high-resolution solid-state NMR spectroscopy of aligned samples.

Authors:  Alexander A Nevzorov; Stanley J Opella
Journal:  J Magn Reson       Date:  2006-10-30       Impact factor: 2.229

3.  Shiftless nuclear magnetic resonance spectroscopy.

Authors:  Chin H Wu; Stanley J Opella
Journal:  J Chem Phys       Date:  2008-02-07       Impact factor: 3.488

Review 4.  Probes for high field solid-state NMR of lossy biological samples.

Authors:  Christopher V Grant; Chin H Wu; Stanley J Opella
Journal:  J Magn Reson       Date:  2010-03-31       Impact factor: 2.229

5.  SPINAL modulated decoupling in high field double- and triple-resonance solid-state NMR experiments on stationary samples.

Authors:  Neeraj Sinha; Christopher V Grant; Chin H Wu; Anna A De Angelis; Stanley C Howell; Stanley J Opella
Journal:  J Magn Reson       Date:  2005-08-30       Impact factor: 2.229

6.  Two- and three-dimensional 1H/13C PISEMA experiments and their application to backbone and side chain sites of amino acids and peptides.

Authors:  Z T Gu; S J Opella
Journal:  J Magn Reson       Date:  1999-10       Impact factor: 2.229

7.  Proton-detected separated local field spectroscopy.

Authors:  Chin H Wu; Stanley J Opella
Journal:  J Magn Reson       Date:  2007-10-10       Impact factor: 2.229

8.  Tailoring 13C labeling for triple-resonance solid-state NMR experiments on aligned samples of proteins.

Authors:  Neeraj Sinha; Fabian V Filipp; Lena Jairam; Sang Ho Park; Joel Bradley; Stanley J Opella
Journal:  Magn Reson Chem       Date:  2007-12       Impact factor: 2.447

9.  Structure of the coat protein in fd filamentous bacteriophage particles determined by solid-state NMR spectroscopy.

Authors:  Ana Carolina Zeri; Michael F Mesleh; Alexander A Nevzorov; Stanley J Opella
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-15       Impact factor: 11.205

10.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
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  4 in total

1.  Structure determination of a membrane protein in proteoliposomes.

Authors:  Bibhuti B Das; Henry J Nothnagel; George J Lu; Woo Sung Son; Ye Tian; Francesca M Marassi; Stanley J Opella
Journal:  J Am Chem Soc       Date:  2012-01-23       Impact factor: 15.419

2.  1H assisted 13C/15N heteronuclear correlation spectroscopy in oriented sample solid-state NMR of single crystal and magnetically aligned samples.

Authors:  Eugene C Lin; Stanley J Opella
Journal:  J Magn Reson       Date:  2011-04-09       Impact factor: 2.229

3.  Experiments optimized for magic angle spinning and oriented sample solid-state NMR of proteins.

Authors:  Bibhuti B Das; Eugene C Lin; Stanley J Opella
Journal:  J Phys Chem B       Date:  2013-10-07       Impact factor: 2.991

4.  Untuned broadband spiral micro-coils achieve sensitive multi-nuclear NMR TX/RX from microfluidic samples.

Authors:  Hossein Davoodi; Nurdiana Nordin; Hirokazu Munakata; Jan G Korvink; Neil MacKinnon; Vlad Badilita
Journal:  Sci Rep       Date:  2021-04-08       Impact factor: 4.379
  4 in total

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