Literature DB >> 21543244

1H assisted 13C/15N heteronuclear correlation spectroscopy in oriented sample solid-state NMR of single crystal and magnetically aligned samples.

Eugene C Lin1, Stanley J Opella.   

Abstract

(1)H-irradiation under mismatched Hartmann-Hahn conditions provides an alternative mechanism for carrying out (15)N/(13)C transfers in triple-resonance heteronuclear correlation spectroscopy (HETCOR) on stationary samples of single crystals and aligned samples of biopolymers, which improve the efficiency especially when the direct (15)N-(13)C dipolar couplings are small. In many cases, the sensitivity is improved by taking advantage of the (13)C(α) labeled sites in peptides and proteins with (13)C detection. The similarities between experimental and simulated spectra demonstrate the validity of the recoupling mechanism and identify the potential for applying these experiments to virus particles or membrane proteins in phospholipid bilayers; however, further development is needed in order to derive quantitative distance and angular constraints from these measurements.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21543244      PMCID： PMC3236683          DOI： 10.1016/j.jmr.2011.03.020

Source DB:  PubMed          Journal:  J Magn Reson        ISSN： 1090-7807            Impact factor:   2.229


  27 in total

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2.  A solid-state NMR index of helical membrane protein structure and topology.

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Journal:  J Magn Reson       Date:  2000-12       Impact factor: 2.229

4.  Mismatched Hartmann-Hahn conditions cause proton-mediated intermolecular magnetization transfer between dilute low-spin nuclei in NMR of static solids.

Authors:  Alexander A Nevzorov
Journal:  J Am Chem Soc       Date:  2008-08-02       Impact factor: 15.419

5.  Three-dimensional solid-state NMR experiment that correlates the chemical shift and dipolar coupling frequencies of two heteronuclei.

Authors:  A Ramamoorthy; C H Wu; S J Opella
Journal:  J Magn Reson B       Date:  1995-04

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Journal:  Biochemistry       Date:  2010-09-07       Impact factor: 3.162

7.  On the performance of spin diffusion NMR techniques in oriented solids: prospects for resonance assignments and distance measurements from separated local field experiments.

Authors:  Nathaniel J Traaseth; T Gopinath; Gianluigi Veglia
Journal:  J Phys Chem B       Date:  2010-11-04       Impact factor: 2.991

8.  Dipolar waves as NMR maps of protein structure.

Authors:  Michael F Mesleh; Gianluigi Veglia; Tara M DeSilva; Francesca M Marassi; Stanley J Opella
Journal:  J Am Chem Soc       Date:  2002-04-24       Impact factor: 15.419

9.  1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins.

Authors:  Eugene C Lin; Chin H Wu; Yuan Yang; Christopher V Grant; Stanley J Opella
Journal:  J Magn Reson       Date:  2010-07-01       Impact factor: 2.229

10.  NMR studies of p7 protein from hepatitis C virus.

Authors:  Gabriel A Cook; Stanley J Opella
Journal:  Eur Biophys J       Date:  2009-09-02       Impact factor: 1.733
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  5 in total

Review 1.  Structure determination of membrane proteins in five easy pieces.

Authors:  Francesca M Marassi; Bibhuti B Das; George J Lu; Henry J Nothnagel; Sang Ho Park; Woo Sung Son; Ye Tian; Stanley J Opella
Journal:  Methods       Date:  2011-09-20       Impact factor: 3.608

2.  A spectroscopic assignment technique for membrane proteins reconstituted in magnetically aligned bicelles.

Authors:  Wenxing Tang; Robert W Knox; Alexander A Nevzorov
Journal:  J Biomol NMR       Date:  2012-09-14       Impact factor: 2.835

3.  Mechanism of dilute-spin-exchange in solid-state NMR.

Authors:  George J Lu; Stanley J Opella
Journal:  J Chem Phys       Date:  2014-03-28       Impact factor: 3.488

4.  Structure determination of a membrane protein in proteoliposomes.

Authors:  Bibhuti B Das; Henry J Nothnagel; George J Lu; Woo Sung Son; Ye Tian; Francesca M Marassi; Stanley J Opella
Journal:  J Am Chem Soc       Date:  2012-01-23       Impact factor: 15.419

Review 5.  The magic of bicelles lights up membrane protein structure.

Authors:  Ulrich H N Dürr; Melissa Gildenberg; Ayyalusamy Ramamoorthy
Journal:  Chem Rev       Date:  2012-08-24       Impact factor: 60.622
  5 in total

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