A J O'Neill1. 1. Antimicrobial Research Centre and Institute of Molecular and Cellular Biology, University of Leeds, Leeds, UK. a.j.oneill@leeds.ac.uk
Abstract
AIMS: To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS: Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequenced ancestral strain, Staph. aureus NCTC 8325. PCR amplification and DNA sequencing were employed to determine which of the genetic polymorphisms identified were also present in Staph. aureus 8325-4, a direct derivative of 8325 and the parent strain of SH1000. Aside from known genetic differences between these strains, Staph. aureus SH1000 harboured 15 single-nucleotide polymorphisms compared with 8325 (of which 12 were also found in 8325-4), and a 63-bp deletion upstream of the spa gene not present in either 8325 or 8325-4. CONCLUSIONS: Staphylococcus aureus SH1000 and 8325-4 contain a number of genetic polymorphisms relative to the progenitor strain of the lineage (8325) and to each other. SIGNIFICANCE AND IMPACT OF THE STUDY: The comparative genome sequences of SH1000 and 8325-4 presented here define the genotypes of two key strains in staphylococcal laboratory research and reveal genetic polymorphisms that may impact their phenotypic properties.
AIMS: To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS: Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequenced ancestral strain, Staph. aureus NCTC 8325. PCR amplification and DNA sequencing were employed to determine which of the genetic polymorphisms identified were also present in Staph. aureus 8325-4, a direct derivative of 8325 and the parent strain of SH1000. Aside from known genetic differences between these strains, Staph. aureus SH1000 harboured 15 single-nucleotide polymorphisms compared with 8325 (of which 12 were also found in 8325-4), and a 63-bp deletion upstream of the spa gene not present in either 8325 or 8325-4. CONCLUSIONS:Staphylococcus aureus SH1000 and 8325-4 contain a number of genetic polymorphisms relative to the progenitor strain of the lineage (8325) and to each other. SIGNIFICANCE AND IMPACT OF THE STUDY: The comparative genome sequences of SH1000 and 8325-4 presented here define the genotypes of two key strains in staphylococcal laboratory research and reveal genetic polymorphisms that may impact their phenotypic properties.
Authors: Arya Gupta; Carmine Monteferrante; Dace Rasina; Gundars Leitis; Christopher P Randall; Jennifer H Tomlinson; Aigars Jirgensons; Wil H F Goessens; John P Hays; Alex J O'Neill Journal: Antimicrob Agents Chemother Date: 2016-04-22 Impact factor: 5.191
Authors: Jennifer Campbell; Atul K Singh; Jonathan G Swoboda; Michael S Gilmore; Brian J Wilkinson; Suzanne Walker Journal: Antimicrob Agents Chemother Date: 2012-01-30 Impact factor: 5.191
Authors: Halie K Miller; Ronan K Carroll; Whittney N Burda; Christina N Krute; Jessica E Davenport; Lindsey N Shaw Journal: J Bacteriol Date: 2012-06-08 Impact factor: 3.490
Authors: Kaat De Cremer; Nicolas Delattin; Katrijn De Brucker; Annelies Peeters; Soña Kucharíková; Evelien Gerits; Natalie Verstraeten; Jan Michiels; Patrick Van Dijck; Bruno P A Cammue; Karin Thevissen Journal: Antimicrob Agents Chemother Date: 2014-10-06 Impact factor: 5.191