| Literature DB >> 20541999 |
Kyoko L Yap1, Side Li, Ana M Muñoz-Cabello, Selina Raguz, Lei Zeng, Shiraz Mujtaba, Jesús Gil, Martin J Walsh, Ming-Ming Zhou.
Abstract
Expression of the INK4b/ARF/INK4a tumor suppressor locus in normal and cancerous cell growth is controlled by methylation of histone H3 at lysine 27 (H3K27me) as directed by the Polycomb group proteins. The antisense noncoding RNA ANRIL of the INK4b/ARF/INK4a locus is also important for expression of the protein-coding genes in cis, but its mechanism has remained elusive. Here we report that chromobox 7 (CBX7) within the polycomb repressive complex 1 binds to ANRIL, and both CBX7 and ANRIL are found at elevated levels in prostate cancer tissues. In concert with H3K27me recognition, binding to RNA contributes to CBX7 function, and disruption of either interaction impacts the ability of CBX7 to repress the INK4b/ARF/INK4a locus and control senescence. Structure-guided analysis reveals the molecular interplay between noncoding RNA and H3K27me as mediated by the conserved chromodomain. Our study suggests a mechanism by which noncoding RNA participates directly in epigenetic transcriptional repression. Copyright (c) 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20541999 PMCID： PMC2886305 DOI： 10.1016/j.molcel.2010.03.021
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970