Fruits and vegetables consumption and the risk of histological subtypes of lung cancer in the European Prospective Investigation into Cancer and Nutrition (EPIC).

OBJECTIVE: To examine the association between fruit and vegetable consumption and risk of different histological subtypes of lung cancer among participants of the European Prospective Investigation into Cancer and Nutrition study.
METHODS: Multivariable Cox proportional hazard models were used to analyze the data. A calibration study in a subsample was used to reduce dietary measurement errors.
RESULTS: During a mean follow-up of 8.7 years, 1,830 incident cases of lung cancer (574 adenocarcinoma, 286 small cell, 137 large cell, 363 squamous cell, 470 other histologies) were identified. In line with our previous conclusions, we found that after calibration a 100 g/day increase in fruit and vegetables consumption was associated with a reduced lung cancer risk (HR 0.94; 95% CI 0.89-0.99). This was also seen among current smokers (HR 0.93; 95% CI 0.90-0.97). Risks of squamous cell carcinomas in current smokers were reduced for an increase of 100 g/day of fruit and vegetables combined (HR 0.85; 95% CI 0.76-0.94), while no clear effects were seen for the other histological subtypes.
CONCLUSION: We observed inverse associations between the consumption of vegetables and fruits and risk of lung cancer without a clear effect on specific histological subtypes of lung cancer. In current smokers, consumption of vegetables and fruits may reduce lung cancer risk, in particular the risk of squamous cell carcinomas.

Introduction

Lung cancer is one of the most common cancers in men. Age-adjusted rates of lung cancer are decreasing among men in many high-income countries due to decreased smoking, but increasing in some low-income countries. In women, incidence rates are lower (globally, the age-standardized incidence rate is 12.1 per 100,000 women compared with 35.5 per 100,000 men), but rates among women continue to rise in many countries [1-3].

Lung cancer can be divided into four major histological subtypes: adenocarcinoma, small cell carcinoma, large cell carcinoma, and squamous cell carcinoma. Squamous cell carcinoma is the predominant histological type among men while in women adenocarcinoma is the most common subtype. The trends in subtypes of lung cancer incidence also vary by gender. In men, the incidence of squamous and small cell cancer is decreasing, while the incidence of adenocarcinoma is stable or slightly increasing in western countries. For women, the incidence of all histological subtypes is increasing, although most rapidly for adenocarcinoma [1].

The major risk factor for lung cancer is tobacco smoking [1, 4]. Tobacco smoking is related to all histological subtypes of lung cancer, but the strength of the association differs with small cell carcinoma showing the strongest association followed by squamous cell carcinoma, while adenocarcinoma shows the weakest association with tobacco smoking [4]. Likewise, the effect of smoking cessation is the strongest for small cell carcinoma and the weakest for adenocarcinoma [5].

Vegetable and fruit consumption have also been hypothesized to influence lung cancer risk [6]. The 2007 WCRF/AICR expert report, ‘Food, Nutrition, Physical Activity, and the Prevention of Cancer: a Global Perspective’, concludes that fruits probably protect against lung cancer and that there is only limited evidence suggesting that non-starchy vegetables, selenium, and foods containing it protect against lung cancer. The 2007 WCRF/AICR expert report does not mention differences in effect of fruit and vegetable consumption between the different histological subtypes of lung cancer [2]. There are indications that the association of vegetables and fruits may vary among the histological subtypes of lung cancer, but study results are inconsistent. A suggestion of a stronger inverse association for total fruits and vegetable consumption and total fruits in adenocarcinomas and squamous cell carcinomas compared to small cell carcinomas was shown in a pooled analysis of eight prospective studies [7].

The purpose of this article is to describe the associations between fruit and vegetable consumption and risks of the different histological subtypes of lung cancer among participants in the European Prospective Investigation into Cancer and nutrition (EPIC) study. The relation between total lung cancer incidence and fruit and vegetable consumption was previously investigated within EPIC by Miller et al. [6] and Linseisen et al. [8]. They both found a reduced risk for lung cancer with a high consumption of fruit [6, 8]. Linseisen et al. [8], using a substantially larger number of cases than Miller et al. [6] (1,126 vs. 860 lung cancer cases), also found a reduced lung cancer risk with a high vegetable consumption in current smokers. With new follow-up data available, for the first time an adequate number of 1,830 lung cancer cases is available for the analyses of the association between fruit and vegetable consumption and risk of histological subtypes of lung cancer, overall and by smoking status.

Methods and materials

Study participants

The European Prospective Investigation into Cancer and Nutrition (EPIC) is an ongoing multicenter cohort study designed to investigate the relations between diet, lifestyle and environmental factors, and the incidence of cancer. The total cohort consists of cohorts of men and women recruited in 23 centers in 10 European countries: Denmark, France, Germany, Greece, Italy, the Netherlands, Norway, Spain, Sweden, and the United Kingdom. The populations and methods have been described in full elsewhere [9]. In brief, the EPIC cohort consists of 521,468 subjects, mostly aged 25–70 years, recruited during the period 1991–2000 from the general population residing in a specific geographic area, a town or a province. Exceptions were the French cohort, which was based on members of the health insurance for state school employees, the Utrecht (the Netherlands) and the Florence (Italy) cohorts, which were both based on women attending breast cancer screening, components of the Italian and Spanish cohorts which included members of local blood donor organizations, and half of the Oxford (United Kingdom) cohort that was based on vegetarian and health-conscious volunteers. In France, Norway, Utrecht (the Netherlands), and Naples (Italy) only women were recruited. Eligible subjects were invited to participate in the study by mail or by personal contact. As a rule, those who participated signed an informed consent form, and diet and lifestyle questionnaires were mailed to them, except in all Spanish centers, Greece, and Ragusa (Italy), where interviewer-administered questionnaires were used. In most countries, study subjects were invited to visit a center for blood collection and anthropometric measurements and to deliver the completed diet and lifestyle questionnaires [9].

Diet and lifestyle questionnaires

At baseline, usual diet before enrollment was measured by country-specific validated questionnaires designed to capture local dietary habits. Although the design of the questionnaires was based on the same general format, there were differences between the questionnaires used in several countries. Extensive self-administered quantitative dietary questionnaires were used in northern Italy, the Netherlands, Germany, and Greece. In France, Spain, and Ragusa (Italy), questionnaires similar to the dietary questionnaires, but structured by meals, were used. To increase the compliance, the centers in Spain and Ragusa performed a face-to-face dietary interview using a computerized dietary program. Semi-quantitative food frequency questionnaires with the same standard portion assigned to all participants were used in Denmark, Norway, Naples (Italy), and Umeå (Sweden). In Malmö (Sweden), a non-quantitative food frequency questionnaire was combined with a 14-day record on hot meals, and in the United Kingdom a semi-quantitative food frequency questionnaire and a 7-day record were used [9].

The food groups analyzed were vegetables and fresh fruits (excluding olives, nuts, seeds, and fruit juices). Analyses were also carried out for subgroups of vegetables (leafy vegetables, fruiting vegetables, cabbages, root vegetables, mushrooms, and garlic and onions) and subgroups of fresh fruits (hard fruit (including apples and pears), stone fruit (including cherry, mirabelle, plum, apricot, peach, and nectarine), berries, grapes, and citrus fruit (excluding and including citrus juices)). Additionally, legumes (including grain and pod vegetables) are analyzed as a separate group. Details of food items included in the selected vegetables and fruits subgroups used in the analysis have been reported in full by Agudo et al. [10].

Lifestyle questionnaires included questions on education, occupation, medical history, lifetime history of consumption of tobacco, alcoholic beverages, and physical activity [9].

Endpoints

Follow-up was based on population-based cancer registries in seven of the participating countries: Denmark, Italy, Netherlands, Spain, Sweden, United Kingdom, and Norway. In France, Germany, and Greece, a combination of methods was used, including health insurance records, cancer and pathology hospital registries, and active follow-up. Mortality data were also collected from registries at the regional or national level [9]. Censoring dates for complete follow-up were as follows: December 2002 (Granada); December 2003 (Florence, Varese, Naples, Murcia, Bilthoven and Denmark); December 2004 (Ragusa, Turin, Asturias, Navarra, United Kingdom, Utrecht, Malmo and Norway); June 2005 (France); December 2005 (San Sebastian and Umea). For Germany and Greece, the end of follow-up was considered to be the last known contact, the date of diagnosis or the date of death, whichever came first.

Cancer of the lung was defined as code C34 of the 10th revision of the International Statistical Classification of Diseases, Injuries, and Causes of Death (ICD). According to the morphology codes of the WHO International Histological Classification of Tumors, histological types were classified into four major histological types: squamous cell carcinoma (8052, 8070–8073, 8075, and 8123), small cell carcinoma (8041–8045 and 8246), large cell carcinoma (8012, 8020–8021, and 8082), and adenocarcinoma (8140, 8143, 8200, 8211, 8230, 8250–8251, 8260, 8300, 8310, 8480–8481, 8490, and 8550). Other histological types (8010–8011, 8022, 8030–8032, 8046, 8240, 8243, 8430, 8560, 8710, 8720, 8800–8801, 9120, 9133, 9590, 9591, 9671, and 9699) and unclassified histological types of carcinomas (8000–8001 and missing histological data) were placed into a miscellaneous category. Only first incident lung cancer cases were taken into account.

Statistical methods

Cox proportional hazards regression was used to analyze the association between fruit and vegetable consumption and risk of different histological subtypes of lung cancer. Age was used as the primary time variable in the models with entry time defined as age at recruitment and exit time as age at diagnosis, age at death, or age at end of follow-up, whichever came first. All analyses were stratified by age at recruitment (in 1-year categories) to control for length of follow-up, and by gender and center to control for country effects such as follow-up procedures, and questionnaire design. The proportional hazard assumption, that was tested by introducing an interaction term between time and the exposure variable, was met. Cases diagnosed after censoring date were considered as non-cases. When analyzing the different histological subgroups of lung cancers, the histological subtypes not of interest were censored at time of diagnosis.

Consumption of vegetables and fruits and of vegetable and fruit subgroups was divided into EPIC-wide quintiles, using the lowest quintile as reference category. The consumption of vegetables and fruits was also analyzed continuously (per 100 g/day increase). Subgroups of vegetables and fruit were analyzed per 25 g/day increase. Analyses were also performed separately by smoking status and by gender. Interaction (on the multiplicative scale) was tested using the interaction term of fruit and/or vegetable consumption (in quintiles) with gender and smoking status.

We controlled, in the overall model, for smoking status (current, former, never), duration of smoking (continues in years; former and current smokers), lifetime intensity of smoking (continues in cigarettes/day; former and current smokers), the number of cigarettes smoked at baseline (continues in cigarettes/day; current smokers), and time since quitting (continues in years; former smokers). Additionally, we included the number of cigarettes at baseline squared and two interaction terms, one for the duration of smoking and the number of cigarettes at baseline and one for the duration of smoking and age at start of smoking. Individuals with unknown smoking status (n = 7164; 1.5%) were excluded from the Cox regression analyses. Indicator variables were used for missing values related to the intensity (20% missing values), duration (5% missing values), and age at start of smoking (3% missing values). All models additionally included height (continues in cm), weight (continues in kg), energy intake from fat and non-fat sources (continues in kcal/day), alcohol consumption (continues in g/day), physical activity (inactive, moderately inactive, moderately active, active, missing), and highest educational level (none, primary school, technical/professional school, secondary school, university). Within the analyses of fruits, we also adjusted for the intake of vegetables and vice versa (continues in grams/day). When analyzing subgroups of vegetables and fruits, also other vegetables and fruits consumption were controlled for. All covariates were included as separate variables on a continuous scale except when stated differently.

We derived probability values for a linear trend across quintiles from regression models using the median consumption within the quintiles as a continuous variable, hereby taking the unequal distances of the quintiles into account [11].

To evaluate whether preclinical disease may have influenced results, additional analyses were conducted after exclusion of cases that were diagnosed within 2 years after recruitment. To separate early from late effects in the natural course, we conducted analyses stratified by median follow-up.

Calibration

To reduce systematic over- and underestimation of dietary intakes across participating centers and to reduce measurement bias in hazard ratios [12, 13], a calibration method was additionally applied as described in detail by Ferrari et al. 2008 [13]. In brief, 24-h recall data were collected from an 8% sample of the cohort. The 24-h recall values were regressed on the dietary questionnaire values for the main food groups and the subgroups in a linear calibration model [13]. Zero consumption values in the main dietary questionnaires were included in the regression calibration models. Data were weighted by day of the week and season of the year on which the 24-h recall data were collected. Country and sex-specific calibration models were used to obtain individual calibrated values of dietary exposure for all participants. Cox regression models were then applied using the calibrated values for each individual on a continuous scale. The standard error of the deattenuated coefficient was calculated with bootstrap sampling (n = 20 repetitions) in the calibration and disease models consecutively [13].

All analyses were performed using SAS version 9.1 (SAS Institute Inc. Cary, NC).

Results

For the analyses, we excluded participants with a history of cancer at baseline (n = 23,633), participants with incomplete follow-up information (n = 3,446), or participants with a ratio of energy intake versus energy expenditure in the top and bottom 1% (n = 15,834). After these exclusions, there were no individuals with missing dietary data. A total of 478,535 participants were left for analyses.

After a mean follow-up of 8.7 years, 1,830 participants were newly diagnosed with a first incident lung cancer, i.e., 574 were classified as adenocarcinomas, 286 as small cell carcinomas, 137 as large cell carcinomas, and 363 as squamous cell carcinomas; 256 cases had other specified histologies and the histology was not specified for 214 participants. Eighty percent of the tumors were microscopically confirmed of which 82% histologically confirmed (65% of the total number of cases).

Table 1 shows the frequency of lung cancers included in the analysis by country and gender. Adenocarcinomas were more common in women (41%) than in men (28%), while squamous cell carcinomas were more common among men (27%) than among women (14%). Overall, 89% of the lung cancer cases were ever smokers; 98% of the small cell carcinomas were ever smokers versus 87% of the adenocarcinoma cases.

Table 1

Incidence of lung cancer within the EPIC cohort, 1993–1998

Country	Person years	First incident lung cancer	Incidence rate per 100,000 person yearsa	Histology of the tumor n (%)
				Adenocarcinoma	Small cell carcinoma	Squamous cell carcinoma	Large cell carcinoma	Other histologies	Not specified
Women
France	7,41,203	129	18.4	4	0	0	0	1	124
Italy	2,57,356	60	29.2	29	7	9	0	9	6
Spain	2,41,319	23	10.3	12	0	1	8	2	0
Greece	1,08,501	11	2.1	4	1	0	1	2	3
United Kingdom	4,41,686	100	29.4	38	11	15	2	26	8
The Netherlandsb	2,28,924	99	45.0	41	16	16	16	9	1
Germany	2,27,267	41	24.9	18	7	3	2	10	1
Sweden	2,71,071	116	45.2	47	19	21	23	5	1
Denmark	2,16,031	219	91.1	85	47	30	11	41	5
Norway	2,10,300	68	46.0	29	15	7	0	15	2
Total	2,943,660	866	42.7	307 (41%)c	123 (17%)c	102 (14%)c	63 (9%)c	120 (16%)c	151 (4%)c
Men
Franced
Italy	1,18,770	77	97.0	27	11	17	4	11	7
Spain	1,53,853	109	86.2	29	18	30	16	9	7
Greece	73,446	79	107.2	18	11	15	1	12	22
United Kingdom	1,90,483	132	50.3	18	15	56	3	36	4
The Netherlandsb	81,362	38	141.6	14	7	11	5	1	0
Germany	1,74,196	145	105.4	42	39	28	5	20	11
Sweden	2,29,535	139	62.6	46	25	32	31	3	2
Denmark	1,95,820	245	116.7	73	37	72	9	44	10
Norwayd
Total	12,17,466	964	76.7	267 (28%)	163 (17%)	261 (27%)	74 (8%)	136 (14%)	63 (7%)

aFor each country (5-year) age-standardized (European standard population) incidence rates were computed for the common age band of 50–69 years of age

bOne of the two Dutch EPIC centers (Utrecht) consists of women only

cPercentages based on data without France because of the large number of non specified tumors

dThe France and Norwegian cohorts consist of women only

Selected characteristics across quintiles of total vegetables and total fruits intake are shown in Table 2. With increasing consumption of vegetables and of fruits, the percentage of women increases. A higher consumption of vegetables and fruits was related to a higher intake of energy but a lower consumption of red and processed meat. Those reporting higher consumption of vegetables and of fruits were more likely to be never smokers and to be physically active.

Table 2

Baseline characteristics by quintiles of observed intake of total vegetables and of total fruits in mean (SD)

	Full cohort	Total vegetable and fruit consumptiona
		1	2	3	4	5
Cut-off values quintiles (g/day)b	–	≤221	222–330	331–453	454–635	≥636
General characteristics
Men (%)	30	44	32	26	22	26
Age at recruitment (year)	51 (9.9)	50 (9.7)	51 (9.8)	51 (9.8)	52 (9.8)	52 (10.4)
BMI (kg/m2)	25.4 (4.3)	25.4 (4.1)	25.3 (4.1)	25.1 (4.1)	25.2 (4.3)	26.0 (4.6)
Height (cm)	166.0 (8.9)	169.0 (9.1)	167.2 (8.9)	165.8 (8.7)	164.4 (8.4)	163.5 (8.4)
Weight (kg)	70.2 (13.7)	72.8 (14.1)	70.9 (13.7)	69.3 (13.3)	68.3 (13.1)	69.7 (13.5)
Physically active (%)c	41.0	45.7	46.3	46.1	46.4	52.6
Diet
Energy (kcal/day)	2,084.6 (622.0)	1,909.3 (609.2)	2,006.7 (596.3)	2,068.8 (590.5)	2,142.6 (602.5)	2,295.4 (641.1)
Energy from fat sources (kcal/day)	749.0 (275.1)	694.7 (264.3)	716.7 (258.0)	729.3 (255.0)	754.4 (260.4)	849.8 (307.5)
Energy from non-fat sources (kcal/day)	1,335.6 (407.0)	1,214.6 (395.1)	1,290.0 (383.5)	1,339.5 (383.1)	1,388.2 (398.2)	1,445.5 (433.7)
Calibrated fruit consumption (g/day)	208.9 (108.5)	112.8 (47.4)	159.9 (56.9)	202.1 (66.4)	246.7 (77.3)	323.2 (129.6)
Calibrated vegetable consumption (g/day)	171.2 (54.3)	124.2 (29.0)	146.6 (31.9)	165.4 (36.8)	189.4 (41.9)	230.3 (55.7)
Alcohol non-consumers (%)	7.3	7.1	7.1	6.9	7.8	8.3
Alcohol consumption (g/day)d	6.6	7.1	7.1	6.9	6.5	5.3
Red and processed meat (g/day)	76.8 (51.7)	85.2 (52.7)	82.4 (52.1)	77.2 (51.3)	72.3 (50.2)	66.9 (50.0)
Smoking status
Never smokers (%)	49	38	45	51	55	57
Former smokers (%)	27	26	28	28	26	24
Lifetime number of cigarettes (cig/day)	13.1 (9.3)	13.4 (9.3)	12.3 (8.6)	12.2 (8.4)	12.6 (8.7)	15.1 (10.8)
Smoke duration (years)	18.4 (11.1)	18.5 (11.2)	18.2 (11.1)	18.1 (11.0)	18.0 (10.9)	18.7 (11.1)
Age at start of smoking (years)	18.9 (5.1)	18.1 (5.6)	18.2 (5.8)	18.2 (6.3)	18.1 (6.6)	18.2 (6.8)
Time since quitting smoking (years)	15.2 (10.3)	14.6 (10.1)	15.3 (10.2)	15.5 (10.3)	15.6 (10.3)	14.7 (10.2)
Current smokers (%)	22	34	25	20	17	17
Lifetime number of cigarettes (cig/day)	13.5 (7.5)	14.4 (7.3)	13.0 (6.9)	12.4 (6.9)	12.4 (7.3)	14.4 (8.9)
Smoke duration (years)	30.1 (10.1)	31.2 (9.8)	30.8 (10.0)	30.3 (10.1)	29.4 (10.1)	27.5 (10.7)
Age at start of smoking (years)	19.6 (6.3)	18.4 (5.9)	19.1 (6.6)	19.4 (7.1)	19.7 (7.4)	20.3 (7.7)
Unknown (%)	2	1	1	1	2	2
Education level (%)
None	4	2	2	3	5	9
Primary school	24	29	24	21	20	24
Technical/professional school	23	30	28	23	19	14
Secondary school	23	18	21	25	27	26
University degree	24	20	24	26	26	24
Not specified	2	1	2	2	3	3

aExcluding juices, nuts, seeds, and olives

bCalibrated interquintile range 258.2–486.1 g/day

cPhysically active as defined by the Combined Total Physical Activity Index that categorizes the population into two activity levels based on a cross-tabulation of occupational activity by household and recreational activity

dMedian consumption of alcohol excluding non-consumers

Fruits and vegetables combined

Increasing fruit and vegetable consumption by 100 g/day was associated with a borderline statistically significant hazard ratio (HR) for lung cancer of 0.98 with a 95% confidence interval (95% CI) of 0.96–1.00. After calibration, a 100 g/day increase in fruit and vegetables consumption was associated with a 6% reduction in lung cancer risk. None of the (un)calibrated risk estimates for the histological subtypes of cancer was statistically significant (Table 4). There was no heterogeneity by country (p for interaction with country was 0.94).

Table 4

Fully adjusted hazard ratios for different histological subtypes of lung cancer by increasing observed and calibrated total fruit and vegetable consumption (per 100 g/day) for the full cohort, by smoke status and by gender

	Lung cancer	Adenocarcinoma	Small cell carcinoma	Large cell carcinoma	Squamous cell carcinoma
Full cohort (478,535)	1,830	574	286	137	363
Fruit and vegetables	0.98 (0.96–1.00)	0.97 (0.93–1.02)	0.97 (0.91–1.05)	1.03 (0.94–1.13)	0.96 (0.90–1.02)
Calibrated	0.94 (0.89–0.99)	0.96 (0.88–1.05)	0.95 (0.83–1.09)	0.90 (0.75–1.08)	0.91 (0.81–1.02)
Fruit	0.97 (0.94–1.01)	0.96 (0.90–1.02)	0.94 (0.85–1.03)	1.06 (0.95–1.19)	0.93 (0.86–1.02)
Calibrated	0.94 (0.88–1.01)	0.93 (0.83–1.05)	0.94 (0.79–1.11)	1.01 (0.80–1.26)	0.89 (0.77–1.02)
Vegetables	0.99 (0.94–1.04)	0.99 (0.91–1.08)	1.04 (0.92–1.18)	0.95 (0.79–1.15)	1.00 (0.90–1.12)
Calibrated	0.94 (0.83–1.07)	1.04 (0.84–1.29)	1.03 (0.72–1.45)	0.69 (0.42–1.13)	0.93 (0.71–1.23)
Current smokers (107,415)	1,167	336	235	102	249
Fruit and vegetables	0.96 (0.93–0.99)	0.93 (0.87–0.99)	0.96 (0.88–1.04)	1.03 (0.92–1.14)	0.94 (0.87–1.02)
Calibrated	0.93 (0.90–0.97)	0.95 (0.90–1.01)	0.96 (0.88–1.06)	0.92 (0.76–1.12)	0.85 (0.76–0.94)
Fruit	0.97 (0.93–1.02)	0.94 (0.86–1.03)	0.93 (0.83–1.04)	1.05 (0.93–1.20)	0.92 (0.83–1.03)
Calibrated	0.97 (0.92–1.02)	0.99 (0.91–1.08)	0.97 (0.86–1.10)	0.97 (0.77–1.22)	0.87 (0.77–0.98)
Vegetables	0.94 (0.88–1.01)	0.90 (0.79–1.03)	1.02 (0.87–1.18)	0.95 (0.75–1.20)	0.98 (0.85–1.13)
Calibrated	0.89 (0.82–0.97)	0.89 (0.77–1.02)	0.98 (0.82–1.17)	0.87 (0.55–1.38)	0.85 (0.72–1.00)
Former smokers (127,530)	467	161	45 a	25 a	104
Fruit and vegetables	1.00 (0.96–1.05)	1.01 (0.94–1.09)			1.00 (0.90–1.10)
Calibrated	0.97 (0.83–1.15)	0.95 (0.69–1.30)			0.96 (0.68–1.36)
Fruit	0.97 (0.91–1.04)	0.99 (0.88–1.11)			0.96 (0.84–1.10)
Calibrated	0.95 (0.83–1.08)	0.91 (0.72–1.15)			0.98 (0.77–1.25)
Vegetables	1.04 (0.96–1.13)	1.04 (0.90–1.20)			1.06 (0.88–1.27)
Calibrated	1.06 (0.86–1.30)	1.12 (0.78–1.62)			0.97 (0.63–1.50)
Never smokers (236,426)	187	76 a	2 a	10 a	9 a
Fruit and vegetables	1.00 (0.93–1.07)
Calibrated	1.02 (0.86–1.21)
Fruit	1.01 (0.92–1.11)
Calibrated	1.03 (0.82–1.30)
Vegetables	0.99 (0.87–1.12)
Calibrated	1.00 (0.69–1.43)
Women (335,886)	866	307	123	63	102
Fruit and vegetables	0.97 (0.93–1.01)	0.97 (0.91–1.04)	0.94 (0.84–1.05)		0.91 (0.80–1.04)
Calibrated	0.91 (0.83–0.99)	0.88 (0.75–1.02)	0.92 (0.72–1.17)		0.78 (0.60–1.02)
Fruit	0.96 (0.91–102)	0.97 (0.88–1.06)	0.83 (0.71–0.99)		0.93 (0.78–1.11)
Calibrated	0.91 (0.80–1.03)	0.84 (0.67–1.05)	0.69 (0.47–1.02)		0.88 (0.60–1.29)
Vegetables	0.98 (0.91–1.05)	0.98 (0.87–1.11)	1.12 (0.93–1.34)		0.88 (0.68–1.13)
Calibrated	0.92 (0.77–1.10)	0.94 (0.71–1.25)	1.65 (0.94–2.90)		0.59 (0.35–0.99)
Men (142,649)	964	267	163	74	261
Fruit and vegetables	0.99 (0.96–1.02)	0.97 (0.91–1.04)	1.00 (0.92–1.09)		0.98 (0.91–1.04)
Calibrated	0.91 (0.85–0.98)	0.92 (0.79–1.06)	0.95 (0.78–1.15)		0.91 (0.79–1.05)
Fruit	0.98 (0.94–1.03)	0.95 (0.87–1.05)	1.00 (0.89–1.13)		0.94 (0.85–1.03)
Calibrated	0.92 (0.84–1.00)	0.88 (0.73–1.05)	1.01 (0.81–1.25)		0.87 (0.72–1.04)
Vegetables	1.00 (0.94–1.07)	1.01 (0.88–1.15)	1.00 (0.84–1.18)		1.04 (0.92–1.18)
Calibrated	0.94 (0.81–1.10)	1.10 (0.82–1.48)	0.87 (0.59–1.28)		1.05 (0.76–1.43)

Cox regression model adjusted for smoking status, duration of smoking, lifetime and baseline intensity of smoking, time since quitting, energy intake, weight, height, alcohol consumption, physical activity, and school level

aToo few cases to get reliable results

Among current smokers, a statistically significant inverse association was observed between consumption of fruit and vegetables and lung cancer risk that remained statistically significant after calibration (HR 0.93; 95% CI 0.90–0.97). After calibration, a 100 g/day increase in consumption of fruit and vegetables in current smokers was associated with a 15% reduction in risk of squamous cell carcinoma, while no effects were seen for the other histological subtypes of lung cancer (Table 4).

Fruits

In the categorical analyses, consumption of fruits was statistically significantly inversely associated with lung cancer risk (Table 3). Compared to the lowest quintile, the HR and 95% CI for those in the highest quintile of consumption was 0.80 (0.66–0.96) with a statistically significant test for trend (p-value 0.01). Suggestions of non-significant lower risks with increasing consumption of fruits were found for small cell, squamous cell, and adenocarcinomas.

Table 3

Fully adjusted hazard ratios for different histological subtypes of lung cancer by quintiles (cut point of the quintiles are (g/day): ≤90; 91–155; 156–238; 239–356; ≥357) of observed fruit consumption for the full cohort, by smoke status and by gender

Fruit consumption (gram per day)	Lung cancer	Adenocarcinoma	Small cell carcinoma	Large cell carcinoma	Squamous cell carcinoma
Full cohort (478,535)	1,830	574	286	137	363
Q1	1.00	1.00	1.00	1.00	1.00
Q2	0.97 (0.85–1.11)	1.12 (0.89–1.41)	1.04 (0.76–1.42)	1.00 (0.61–1.63)	1.00 (0.75–1.32)
Q3	0.82 (0.71–0.96)	0.89 (0.68–1.17)	0.74 (0.50–1.10)	0.74 (0.41–1.34)	0.76 (0.54–1.07)
Q4	0.88 (0.74–1.03)	0.91 (0.67–1.22)	0.85 (0.55–1.32)	1.70 (1.00–2.88)	0.82 (0.56–1.20)
Q5	0.80 (0.66–0.96)	0.85 (0.60–1.19)	0.77 (0.46–1.27)	1.07 (0.54–2.14)	0.77 (0.50–1.19)
p for trend	0.01	0.20	0.21	0.39	0.16
Current smokers (107,415)	1,167	336	235	102	249
Q1	1.00	1.00	1.00	1.00	1.00
Q2	0.96 (0.82–1.13)	0.94 (0.70–1.26)	1.16 (0.82–1.62)	1.06 (0.61–1.83)	0.99 (0.71–1.38)
Q3	0.74 (0.61–0.90)	0.84 (0.59–1.20)	0.70 (0.45–1.10)	0.72 (0.36–1.44)	0.67 (0.43–1.04)
Q4	0.89 (0.72–1.10)	0.82 (0.55–1.23)	0.88 (0.53–1.44)	1.88 (1.03–3.45)	0.82 (0.51–1.32)
Q5	0.79 (0.62–1.02)	0.86 (0.54–1.36)	0.85 (0.48–1.51)	0.90 (0.38–2.16)	0.61 (0.34–1.10)
p for trend	0.04	0.39	0.37	0.59	0.07
Former smokers (127,530)	467	161	45 a	25 a	104
Q1	1.00	1.00			1.00
Q2	0.99 (0.75–1.31)	1.71 (1.05–2.76)			1.09 (0.60–1.95)
Q3	0.92 (0.68–1.23)	1.00 (0.58–1.74)			1.17 (0.64–2.15)
Q4	0.77 (0.55–1.08)	0.93 (0.51–1.69)			0.85 (0.41–1.76)
Q5	0.84 (0.59–1.21)	1.09 (0.57–2.09)			1.13 (0.55–2.34)
p for trend	0.24	0.53			0.90
Never smokers (236,426)	187	76	2 a	10 a	9 a
Q1	1.00	1.00
Q2	1.18 (0.67–2.09)	1.36 (0.58–3.22)
Q3	1.24 (0.71–2.15)	1.41 (0.59–3.34)
Q4	1.46 (0.84–2.53)	1.64 (0.68–3.94)
Q5	0.94 (0.50–1.77)	0.80 (0.28–2.31)
p for trend	0.63	0.48
Women (335,886)	866	307	123	63	102
Q1	1.00	1.00	1.00	1.00	1.00
Q2	0.91 (0.75–1.11)	1.01 (0.73–1.39)	1.04 (0.66–1.64)	0.57 (0.26–1.29)	0.95 (0.55–1.63)
Q3	0.77 (0.62–0.96)	0.92 (0.65–1.32)	0.47 (0.25–0.88)	0.86 (0.39–1.93)	0.66 (0.34–1.28)
Q4	0.84 (0.67–1.06)	0.82 (0.55–1.22)	0.61 (0.32–1.15)	1.40 (0.66–2.98)	1.07 (0.57–2.00)
Q5	0.77 (0.59–1.00)	0.80 (0.51–1.27)	0.54 (0.25–1.19)	0.86 (0.31–2.37)	0.76 (0.33–1.76)
p for trend	0.06	0.23	0.04	0.61	0.67
Men (142,649)	964	267	163	74	261
Q1	1.00	1.00	1.00	1.00	1.00
Q2	1.03 (0.86–1.23)	1.25 (0.90–1.74)	0.99 (0.64–1.53)	1.40 (0.76–2.60)	1.02 (0.73–1.42)
Q3	0.88 (0.71–1.08)	0.85 (0.56–1.29)	1.06 (0.65–1.75)	0.54 (0.21–1.36)	0.82 (0.55–1.22)
Q4	0.92 (0.72–1.17)	1.06 (0.68–1.66)	1.16 (0.65–2.09)	1.81 (0.85–3.84)	0.71 (0.43–1.15)
Q5	0.82 (0.63–1.08)	0.93 (0.56–1.56)	1.04 (0.53–2.03)	1.12 (0.43–2.93)	0.77 (0.46–1.30)
p for trend	0.12	0.63	0.82	0.67	0.20

Cox regression model adjusted for vegetable consumption, smoking status, duration of smoking, lifetime and baseline intensity of smoking, time since quitting, energy intake, weight, height, alcohol consumption, physical activity, and school level

aToo few cases to get reliable results

For current smokers, results for fruits were consistent with those of the full cohort (p for trend 0.04), with strongest inverse association found for squamous cell carcinoma (HR 0.61; 95% CI 0.34–1.10 comparing highest quintile with the lowest; p-trend = 0.07). The test for interaction with smoking was borderline significant (p = 0.09). No clear associations in former and never smokers were seen, not overall nor for any of the subtypes of lung cancer for which adequate numbers were available. Stratification by gender showed somewhat stronger associations among women and a significant decreasing risk trend for small cell carcinoma (p for interaction with gender was 0.50).

Increasing fruit consumption with 100 g/day was associated with a small borderline statistically significant lower risk of lung cancer which was more pronounced for squamous cell carcinoma (Table 4). The calibrated risk estimates were somewhat stronger but not statistically significant. Because linear analyses are more sensitive to outliers, we performed a sensitivity analysis for total fruits in the full cohort by substituting values higher than 600 g/day with the value of 600 g/day (95 percentile of total fruit consumption). We found a slightly stronger uncalibrated hazard ratio of 0.96 with 95% CI of 0.92–1.00 (p-value = 0.03). After calibration, higher consumption of fruits was statistically significantly inversely associated with the risk of squamous cell carcinomas in current smokers (Table 4).

Out of all types of fruits tested, after calibration only consumption of berries was inversely associated with risk of lung cancer (HR 0.78; 95% CI 0.63–0.96), with no clear difference between subtypes of lung cancer. An inverse association was found between the consumption of citrus fruits and squamous cell carcinomas in current smokers, which was borderline statistically significant (Table 5).

Table 5

Fully adjusted hazard ratios for different histological subtypes of lung cancer by increasing observed and calibrated, total fruit (per 100 g/day) and fruit subgroup consumption (per 25 g/day) for the full cohort and current smokers separately

	Lung cancer (n = 1,830)	Adenocarcinoma (n = 574)	Small cell carcinoma (n = 286)	Large cell carcinoma (n = 137)	Squamous cell carcinoma (n = 363)
Full cohort
Hard fruit	0.99 (0.97–1.01)	0.99 (0.96–1.02)	1.00 (0.96–1.05)	1.00 (0.95–1.06)	1.01 (0.97–1.04)
Calibrated	0.99 (0.96–1.02)	0.97 (0.92–1.02)	1.01 (0.95–1.09)	1.01 (0.90–1.13)	1.02 (0.96–1.07)
Stone fruita	1.01 (0.97–1.06)	0.97 (0.89–1.06)	1.01 (0.88–1.16)	1.08 (0.92–1.27)	0.97 (0.86–1.10)
Calibrated	0.97 (0.90–1.06)	1.03 (0.91–1.18)	0.93 (0.75–1.15)	0.98 (0.73–1.32)	0.82 (0.68–1.00)
Berriesb	1.07 (0.96–1.19)	1.02 (0.84–1.24)	1.13 (0.89–1.44)	1.10 (0.83–1.45)	1.08 (0.84–1.39)
Calibrated	0.78 (0.63–0.96)	0.76 (0.51–1.13)	0.88 (0.50–1.54)	0.76 (0.29–1.98)	0.72 (0.38–1.35)
Grapesa,c	1.07 (0.99–1.15)	0.98 (0.82–1.18)	1.02 (0.78–1.32)	d	0.92 (0.72–1.18)
Calibrated	1.00 (0.91–1.10)	0.87 (0.71–1.05)	1.02 (0.77–1.36)	d	0.93 (0.75–1.14)
Citrus fruite	0.99 (0.96–1.02)	0.95 (0.90–1.01)	0.95 (0.87–1.02)	1.05 (0.97–1.14)	0.94 (0.87–1.01)
Calibrated	0.99 (0.94–1.04)	0.97 (0.90–1.05)	0.96 (0.86–1.07)	1.05 (0.89–1.23)	0.97 (0.89–1.07)
Citrus fruit incl juiceb,e,f	1.00 (0.98–1.01)	0.98 (0.95–1.01)	0.99 (0.96–1.03)	1.03 (0.98–1.09)	1.00 (0.96–1.04)
Calibrated	1.00 (0.98–1.03)	0.98 (0.93–1.02)	0.99 (0.95–1.04)	1.02 (0.94–1.09)	1.03 (0.99–1.08)
Current smokers
Hard fruit	1.00 (0.98–1.02)	0.99 (0.95–1.04)	1.00 (0.96–1.06)	d	1.02 (0.98–1.07)
Calibrated	0.99 (0.97–1.01)	1.01 (0.97–1.04)	1.01 (0.96–1.06)	d	0.99 (0.95–1.03)
Stone fruita	0.97 (0.91–1.04)	0.94 (0.83–1.08)	1.04 (0.89–1.20)	d	0.96 (0.82–1.13)
Calibrated	0.99 (0.95–1.04)	1.03 (0.96–1.09)	1.03 (0.91–1.17)	d	0.97 (0.88–1.07)
Berriesb	0.97 (0.83–1.12)	0.88 (0.65–1.19)	1.19 (0.91–1.55)	d	0.91 (0.62–1.34)
Calibrated	0.90 (0.78–1.04)	0.85 (0.69–1.04)	1.11 (0.76–1.62)	d	0.87 (0.61–1.26)
Grapesb,c	1.12 (1.03–1.21)	1.10 (0.93–1.31)	1.07 (0.80–1.42)	d	0.99 (0.72–1.38)
Calibrated	0.99 (0.94–1.04)	1.01 (0.93–1.10)	0.89 (0.75–1.05)	d	0.97 (0.85–1.10)
Citrus fruite	0.99 (0.95–1.02)	0.96 (0.89–1.03)	0.96 (0.88–1.05)	d	0.89 (0.80–0.98)
Calibrated	1.01 (0.98–1.03)	0.99 (0.94–1.04)	1.03 (0.94–1.12)	d	0.91 (0.84–1.00)
Citrus fruit incl juiceb,e,f	1.00 (0.98–1.02)	0.98 (0.94–1.02)	0.99 (0.94–1.03)	d	0.99 (0.94–1.04)
Calibrated	1.00 (0.98–1.02)	0.99 (0.96–1.04)	0.98 (0.94–1.02)	d	1.02 (0.97–1.06)

Cox regression model adjusted for vegetable consumption, smoking status, duration of smoking, lifetime and baseline intensity of smoking, time since quitting, energy intake, weight, height, alcohol consumption, physical activity, and school level

aUmea and Norway excluded because of missing data

bUnited Kingdom and Norway excluded because of missing data

cDenmark excluded because of missing data

dToo few cases to get reliable results

eSpain excluded because of missing data

fFrance and Naples excluded because of missing data

To control for potential changes in diet due to preclinical diseases, we excluded the first 2 years of follow-up. The inverse association between fruit consumption and lung cancer risk became somewhat stronger (HR 0.96; 95% CI 0.92–0.99 per 100 g/day increase in consumption) with a calibrated continuous risk estimate of 0.92 (95% CI 0.85–1.00) for each 100 g/d increase in consumption. Analyzing below and above median follow-up (8.5 years) separately showed results comparable to the overall analyses for below median follow-up (uncalibrated HR 0.98, 95% CI 0.94–1.02 per 100 g/day increase in consumption) whereas no association was seen for follow-up periods longer than the mean of 8.7 years (uncalibrated HR 1.00, 95% CI 0.91–1.10 per 100 g/day increase in consumption).

Vegetables

In categorical and continuous analyses, consumption of vegetables was not associated with risk of lung cancer nor with risk of any of the histological subtypes (Tables 4, 6). However, in current smokers after calibration, a statistically significantly inverse association with the consumption of total vegetables was seen, that was borderline statistically significant only for risk of squamous cell carcinomas (Table 4). The test for interaction with smoking was borderline significant (p 0.05).

Table 6

Fully adjusted hazard ratios for different histological subtypes of lung cancer by quintiles (cut point of the quintiles are (g/day): ≤97; 98–146; 147–208; 209–306; ≥307) of observed vegetable consumption for the full cohort, by smoke status and by gender

Vegetable consumption (gram per day)	Lung cancer	Adenocarcinoma	Small cell carcinoma	Large cell carcinoma	Squamous cell carcinoma
Full cohort (478,535)	1,830	574	286	137	363
Q1	1.00	1.00	1.00	1.00	1.00
Q2	1.00 (0.87–1.14)	0.89 (0.70–1.12)	1.17 (0.85–1.62)	0.76 (0.47–1.21)	1.05 (0.78–1.40)
Q3	0.94 (0.82–1.09)	0.86 (0.67–1.11)	1.09 (0.76–1.56)	0.84 (0.51–1.38)	0.83 (0.59–1.16)
Q4	0.94 (0.80–1.11)	0.91 (0.68–1.21)	1.18 (0.77–1.79)	0.81 (0.45–1.46)	0.97 (0.68–1.39)
Q5	0.96 (0.79–1.17)	1.10 (0.78–1.55)	1.17 (0.67–2.02)	0.81 (0.38–1.72)	0.96 (0.62–1.50)
p for trend	0.58	0.66	0.57	0.55	0.75
Current smokers (107,415)	1,167	336	235	102	249
Q1	1.00	1.00	1.00	1.00	1.00
Q2	1.13 (0.96–1.32)	0.98 (0.73–1.31)	1.23 (0.86–1.76)	0.95 (0.57–1.61)	1.31 (0.94–1.82)
Q3	0.90 (0.75–1.08)	0.83 (0.59–1.16)	1.19 (0.80–1.77)	0.90 (0.50–1.62)	0.69 (0.44–1.06)
Q4	0.94 (0.77–1.16)	0.94 (0.64–1.37)	1.41 (0.90–2.21)	0.79 (0.38–1.65)	1.00 (0.64–1.55)
Q5	0.87 (0.66–1.13)	0.89 (0.54–1.48)	0.84 (0.42–1.69)	0.80 (0.30–2.14)	0.87 (0.47–1.59)
p for trend	0.15	0.55	0.87	0.56	0.39
Former smokers (127,530)	467	161	45 a	25 a	104
Q1	1.00	1.00			1.00
Q2	0.79 (0.59–1.07)	0.90 (0.57–1.44)			0.54 (0.27–1.09)
Q3	1.00 (0.74–1.34)	0.85 (0.52–1.40)			1.23 (0.67–2.27)
Q4	0.81 (0.58–1.13)	0.70 (0.40–1.24)			0.89 (0.45–1.78)
Q5	1.04 (0.73–1.49)	1.13 (0.62–2.06)			1.06 (0.51–2.23)
p for trend	0.62	0.77			0.54
Never smokers (236,426)	187	76	2 a	10 a	9 a
Q1	1.00	1.00
Q2	0.59 (0.34–1.02)	0.46 (0.21–1.04)
Q3	0.96 (0.59–1.58)	0.96 (0.48–1.92)
Q4	0.99 (0.59–1.66)	1.04 (0.48–2.24)
Q5	0.81 (0.46–1.45)	1.32 (0.55–3.17)
p for trend	0.90	0.24
Women (335,886)	866	307	123	63	102
Q1	1.00	1.00	1.00	1.00	1.00
Q2	0.85 (0.70–1.05)	0.90 (0.65–1.25)	0.93 (0.57–1.54)	0.50 (0.23–1.12)	0.87 (0.51–1.49)
Q3	0.91 (0.74–1.12)	0.93 (0.66–1.30)	0.81 (0.47–1.40)	1.10 (0.55–2.20)	0.80 (0.44–1.45)
Q4	0.93 (0.74–1.16)	0.98 (0.67–1.44)	0.92 (0.49–1.75)	1.56 (0.72–3.35)	0.95 (0.48–1.85)
Q5	0.89 (0.68–1.17)	1.05 (0.66–1.69)	1.51 (0.71–3.21)	0.95 (0.31–2.97)	0.64 (0.24–1.70)
p for trend	0.60	0.76	0.45	0.41	0.45
Men (142,649)	964	267	163	74	261
Q1	1.00	1.00	1.00	1.00	1.00
Q2	1.14 (0.95–1.36)	0.88 (0.63–1.24)	1.42 (0.92–2.18)	1.02 (0.57–1.83)	1.15 (0.81–1.63)
Q3	0.97 (0.79–1.19)	0.78 (0.53–1.16)	1.38 (0.85–2.24)	0.65 (0.31–1.38)	0.85 (0.57–1.28)
Q4	0.95 (0.75–1.20)	0.84 (0.54–1.30)	1.48 (0.84–2.60)	0.36 (0.13–0.99)	1.01 (0.66–1.54)
Q5	1.04 (0.79–1.37)	1.20 (0.72–1.99)	0.97 (0.45–2.13)	0.65 (0.23–1.81)	1.09 (0.66–1.82)
p for trend	0.89	0.66	0.79	0.14	0.88

Cox regression model adjusted for fruit consumption, smoking status, duration of smoking, lifetime and baseline intensity of smoking, time since quitting, energy intake, weight, height, alcohol consumption, physical activity, and school level

aToo few cases to get reliable results

Only after calibration, consumption of leafy vegetables was inversely associated with lung cancer risk, while no statistically significant associations were seen for histological subtypes of lung cancer (Table 7). Also only after calibration, an increase in consumption of cabbages of 25 g/day was statistically significantly inversely associated with squamous cell carcinomas. In current smokers, none of the subtypes of vegetables was statistically significantly associated with any of the histological subtypes of lung cancer.

Table 7

Fully adjusted hazard ratios for different histological subtypes of lung cancer by increasing observed and calibrated total vegetables (per 100 g/day) and vegetable subgroup consumption (per 25 g/day) for the full cohort and current smokers separately

	Lung cancer (n = 1,830)	Adenocarcinoma (n = 574)	Small cell carcinoma (n = 286)	Large cell carcinoma (n = 137)	Squamous cell carcinoma (n = 363)
Full Cohort
Leafy vegetablesa	0.99 (0.94–1.03)	1.01 (0.93–1.11)	0.96 (0.82–1.13)	1.02 (0.87–1.18)	0.96 (0.85–1.08)
Calibrated	0.85 (0.74–0.98)	0.86 (0.67–1.11)	0.91 (0.62–1.33)	0.78 (0.49–1.25)	0.94 (0.70–1.26)
Fruiting vegetables	1.00 (0.98–1.03)	0.98 (0.94–1.04)	1.04 (0.97–1.10)	0.92 (0.82–1.02)	1.04 (0.98–1.10)
Calibrated	0.99 (0.94–1.05)	0.99 (0.89–1.10)	1.07 (0.92–1.24)	0.81 (0.64–1.02)	1.05 (0.92–1.21)
Cabbagea	1.00 (0.96–1.05)	1.03 (0.94–1.12)	1.01 (0.87–1.14)	0.97 (0.79–1.20)	0.98 (0.89–1.09)
Calibrated	1.06 (0.95–1.18)	1.24 (1.01–1.52)	1.11 (0.84–1.47)	0.87 (0.57–1.34)	0.77 (0.61–0.97)
Root vegetables	1.01 (0.97–1.06)	1.04 (0.97–1.12)	0.96 (0.84–1.09)	1.05 (0.90–1.23)	1.06 (0.97–1.17)
Calibrated	0.99 (0.89–1.11)	1.10 (0.95–1.27)	0.87 (0.68–1.11)	1.03 (0.66–1.60)	1.10 (0.91–1.33)
Mushroomsa,b	0.82 (0.68–1.00)	0.91 (0.64–1.29)	0.97 (0.60–1.57)	0.32 (0.09–1.10)	0.48 (0.28–0.81)
Calibrated	0.96 (0.62–1.50)	0.85 (0.39–1.83)	0.90 (0.32–2.53)	0.23 (0.03–1.98)	0.57 (0.22–1.45)
Onion and Garlica,b,c	0.95 (0.84–1.06)	1.00 (0.82–1.22)	1.05 (0.80–1.38)	0.70 (0.43–1.13)	0.90 (0.70–1.15)
Calibrated	0.92 (0.71–1.18)	1.14 (0.72–1.78)	0.71 (0.36–1.38)	0.59 (0.22–1.55)	0.89 (0.52–1.52)
Current smokers
Leafy vegetablesa	0.97 (0.91–1.04)	1.02 (0.90–1.15)	0.94 (0.79–1.12)	d	0.98 (0.84–1.13)
Calibrated	0.96 (0.90–1.02)	0.92 (0.81–1.05)	1.11 (0.91–1.35)	d	0.96 (0.80–1.14)
Fruiting vegetables	1.01 (0.97–1.04)	0.96 (0.90–1.04)	1.03 (0.95–1.12)	d	1.04 (0.97–1.11)
Calibrated	0.97 (0.94–1.00)	0.97 (0.93–1.02)	0.98 (0.91–1.05)	d	0.98 (0.92–1.04)
Cabbageb	1.00 (0.93–1.08)	1.07 (0.94–1.22)	0.96 (0.81–1.14)	d	0.90 (0.76–1.06)
Calibrated	0.96 (0.91–1.01)	0.99 (0.93–1.06)	1.06 (0.92–1.23)	d	0.94 (0.85–1.04)
Root vegetables	0.97 (0.91–1.04)	0.96 (0.85–1.08)	0.98 (0.85–1.14)	d	1.09 (0.97–1.23)
Calibrated	1.01 (0.94–1.08)	1.01 (0.92–1.10)	0.93 (0.78–1.10)	d	1.05 (0.91–1.21)
Mushroomsa,b	0.75 (0.57–0.99)	0.86 (0.53–1.40)	1.09 (0.63–1.91)	d	0.37 (0.18–0.78)
Calibrated	1.12 (0.86–1.46)	1.03 (0.58–1.83)	0.88 (0.42–1.84)	d	0.90 (0.48–1.67)
Onion and Garlica,b,c	0.95 (0.82–1.10)	0.98 (0.75–1.27)	1.13 (0.85–1.51)	d	1.00 (0.75–1.34)
Calibrated	0.98 (0.86–1.11)	0.93 (0.75–1.15)	1.01 (0.75–1.38)	d	1.04 (0.82–1.32)

Cox regression model adjusted for fruit consumption, smoking status, duration of smoking, lifetime and baseline intensity of smoking, time since quitting, energy intake, weight, height, alcohol consumption, physical activity, and school level

aNorway excluded because of missing data

bUmea excluded because of missing data

cFrance excluded because of missing data

dToo few cases to get reliable results

The results for vegetable consumption did not change when we excluded the first 2 years of follow-up nor when we analyzed the cohort below and above median follow-up (8.5 years) separately (data not shown).

Discussion

In line with the previous EPIC study on fruit and vegetable consumption and lung cancer risk [8], we too found inverse associations between the consumption of vegetables and fruits combined and of fruits and risk of lung cancer. In this study, we additionally looked at fruit and vegetable consumption and the different histological subtypes of lung cancer. However, we did not see a clear effect of consumption of fruit and/or vegetables on risks for specific histological subtypes of lung cancer. In current smokers, we found that the consumption of vegetables and fruits combined and separately may reduce lung cancer risk, in particular the risk of squamous cell carcinoma. Several inverse associations between subgroups of fruits and of vegetables and risk of (types of) lung cancer were seen, i.e., between leafy vegetables and berries and overall lung cancer; between cabbages and squamous cell carcinomas; in current smokers between citrus fruits and squamous cell carcinomas.

The 2007 WCRF/AICR expert report, including only the first EPIC publication on fruit and vegetable consumption and lung cancer risk by Miller et al. [6], concluded that the evidence of a inverse relationship between increased fruit consumption and decreased lung cancer risk is consistent and that there is a dose–response relationship both found in cohort and case–control studies [2]. Our study is in line with these findings. Intake of fruits was inversely related to the risk of lung cancer in categorical but not continuous analyses, but this difference could be explained by the effect of outliers. Out of 6 types of fruits, only intake of berries was inversely related to lung cancer risk. Two previous Finish cohort studies found opposite results for the effect of berries on lung cancer risk [14, 15]. On the other hand, studies on the intake of vegetables and risk of total lung cancer have been inconsistent. Some found an inverse association while others have not been able to reproduce these findings. A protective effect of total vegetable consumption was also not observed in the study by Miller et al. and other previous investigations [6, 16–19]. Vegetables are generally considered as food items that are not very easy to assess in food frequency questionnaires (as well as in other methods of dietary assessment). Indeed, within the EPIC validation studies, the correlation coefficients for total vegetable consumption were in general lower than those for fruits [20]. In our study, no evidence was seen for the consumption of total vegetables and overall lung cancer risk with HR 0.99 and 95% CI 0.94–1.04. After calibration, this effect was somewhat stronger but still not statistically significant. However, after calibration, we did find a 15% decrease in lung cancer risk with an increased consumption of leafy vegetables of 25 g/day. This is in agreement with the meta-analysis based on three cohort studies [16, 21] performed by WCRF/AICR that found an overall RR of 0.91 (95% CI 0.90–0.93) with increments of 1 serving/day of green leafy vegetables, although this result mainly depends on one large cohort study from Japan [21]. The expert panel of WCRF/AICR also found substantial evidence that food containing carotenoids, like carrots, probably protect against lung cancer. However, we did not find any association between increased root vegetable consumption and lung cancer risk.

Due to a larger number of cases, we were able to focus on risks of histological subgroups of lung cancer. Few previous cohort studies have analyzed the effect of fruits and vegetables on different histological subtypes of lung tumors [7, 22–24]. Most studies have divided lung tumors into two groups; Kreyberg I (comprising small cell carcinomas, squamous cell carcinomas, and large cell carcinomas) and Kreyberg II (adenocarcinomas). There were indications that fruits and vegetables were more protective for non-adenocarcinomas (Kreyberg I) than for adenocarcinomas (Kreyberg II). Voorrips et al. [22] found a protective effect of high intake of fruits in Kreyberg I tumors. In the study of Skuladottir et al. [23], a protective effect was found for fruits on squamous cell carcinomas, and an inverse association was found for vegetables on small cell carcinomas. No association for the intake of fruits and vegetables and any histological subtypes of lung cancer were reported by Liu et al. [17] and Feskanich et al.[16]. Some case–control studies have also reported that vegetables play a more beneficial role for non-adenocarcinomas than for adenocarcinomas [25, 26]. Still, it is difficult to compare the studies because of different classifications for lung cancer subtypes, and because most studies have only a small number of cases for analyses. In our study, only 80% of the tumors were microscopically confirmed of which 82% histologically. This left us with 470 cases that could not be categorized in one of the four histological subgroups of lung cancer. Higher microscopic and histological confirmation rates would have given our analyses more power. We did not observe statistically significant inverse associations between consumption of fruit and/or vegetables and risks of the histological subtypes of lung cancer.

Many studies (retrospective and prospective) have indicated a clear protective effect of fruit and vegetables on lung cancer risk among current smokers only [7]. These studies suggested that antioxidants from vegetables and fruits strongly reduce the oxidative stress due to smoking. By contrast, others have found a stronger protective effect of fruits among non-smokers. It is argued that the inverse association among current smokers seen in some studies might be due to residual confounding by smoking [23]. In our study, among current smokers, inverse effects were seen for fruit and vegetables consumption combined and separately and lung cancer risk, which is in line with the previous study of Linseisen et al. 2007 [8] within the EPIC cohort and with the pooled analyses of cohort studies published by Smith-Warner et al. 2003 [7]. Additionally, we were able to classify risks by smoking status. In current smokers, statistically (borderline) significant inverse associations emerged between the consumption of fruit and vegetables combined and separately, and of citrus fruits and risk of squamous cell carcinomas, the type of lung cancer most strongly related to smoking.

Important advantages of our cohort study are its size and the large heterogeneity of fruit and vegetable consumption, caused by the inclusion of participants living in countries from the north to the south of Europe. However, despite using EPIC-wide cut points for construction of categories because of methodological differences between the participating countries, analyses were stratified by study center. To some extent, this counteracts the advantage of the large heterogeneity in dietary exposures.

Although evidence is accumulating that a diet rich in vegetables and fruits may indeed protect against lung cancer especially risk of squamous cell carcinoma in current smokers, the many associations tested in our study need to be replicated in independent large cohort studies to further investigate the role of types of fruit and vegetables in the development of histological subtypes of lung cancer.

In conclusion, we found inverse associations between the consumption of vegetables and fruits combined and of fruits and risk of lung cancer without a clear effect on risks for histological subtypes of lung cancer. In current smokers, consumption of vegetables and fruits combined and separately may reduce lung cancer risk, in particular risk of squamous cell carcinomas although residual confounding by smoking cannot be ruled out.