Literature DB >> 19668194

Cell-selective metabolic labeling of proteins.

John T Ngo1, Julie A Champion, Alborz Mahdavi, I Caglar Tanrikulu, Kimberly E Beatty, Rebecca E Connor, Tae Hyeon Yoo, Daniela C Dieterich, Erin M Schuman, David A Tirrell.   

Abstract

Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

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Year:  2009        PMID： 19668194      PMCID： PMC3176724          DOI： 10.1038/nchembio.200

Source DB:  PubMed          Journal:  Nat Chem Biol        ISSN： 1552-4450            Impact factor:   15.040


  12 in total

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Review 3.  Metabolic labeling of proteins for proteomics.

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Review 4.  Functional and quantitative proteomics using SILAC.

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Review 5.  Chemistry in living systems.

Authors:  Jennifer A Prescher; Carolyn R Bertozzi
Journal:  Nat Chem Biol       Date:  2005-06       Impact factor: 15.040

6.  Fluorescence visualization of newly synthesized proteins in mammalian cells.

Authors:  Kimberly E Beatty; Julie C Liu; Fang Xie; Daniela C Dieterich; Erin M Schuman; Qian Wang; David A Tirrell
Journal:  Angew Chem Int Ed Engl       Date:  2006-11-13       Impact factor: 15.336

7.  Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids.

Authors:  A James Link; Mandy K S Vink; Nicholas J Agard; Jennifer A Prescher; Carolyn R Bertozzi; David A Tirrell
Journal:  Proc Natl Acad Sci U S A       Date:  2006-06-26       Impact factor: 11.205

8.  Selective dye-labeling of newly synthesized proteins in bacterial cells.

Authors:  Kimberly E Beatty; Fang Xie; Qian Wang; David A Tirrell
Journal:  J Am Chem Soc       Date:  2005-10-19       Impact factor: 15.419

9.  Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging.

Authors:  Daniela C Dieterich; Jennifer J Lee; A James Link; Johannes Graumann; David A Tirrell; Erin M Schuman
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

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Authors:  Christian W Tornøe; Caspar Christensen; Morten Meldal
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  76 in total

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Review 3.  Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications.

Authors:  Jeremiah A Johnson; Ying Y Lu; James A Van Deventer; David A Tirrell
Journal:  Curr Opin Chem Biol       Date:  2010-11-09       Impact factor: 8.822

Review 4.  Proteomics of the Synapse--A Quantitative Approach to Neuronal Plasticity.

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Review 5.  Cell-selective proteomics for biological discovery.

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Journal:  Curr Opin Chem Biol       Date:  2017-01-12       Impact factor: 8.822

Review 6.  Optimizing non-natural protein function with directed evolution.

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Review 9.  The Growing Toolbox for Protein Synthesis Studies.

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10.  Discrimination of colon cancer stem cells using noncanonical amino acid.

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Journal:  Chem Commun (Camb)       Date:  2012-07-30       Impact factor: 6.222
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