| Literature DB >> 19441086 |
Mustafa K Khokha1, Vladimir Krylov, Michael J Reilly, Joseph G Gall, Dipankan Bhattacharya, Chung Yan J Cheung, Sarah Kaufman, Dang Khoa Lam, Jaroslav Macha, Catherine Ngo, Neha Prakash, Philip Schmidt, Tereza Tlapakova, Toral Trivedi, Lucie Tumova, Anita Abu-Daya, Timothy Geach, Elisenda Vendrell, Holly Ironfield, Ludivine Sinzelle, Amy K Sater, Dan E Wells, Richard M Harland, Lyle B Zimmerman.
Abstract
Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (c) 2009 Wiley-Liss, Inc.Entities:
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Year: 2009 PMID： 19441086 PMCID： PMC2962985 DOI： 10.1002/dvdy.21965
Source DB: PubMed Journal: Dev Dyn ISSN： 1058-8388 Impact factor: 3.780