Literature DB >> 19246005

Photocleavage of the polypeptide backbone by 2-nitrophenylalanine.

Francis B Peters1, Ansgar Brock, Jiangyun Wang, Peter G Schultz.   

Abstract

Photocleavage of the polypeptide backbone is potentially a powerful and general method to activate or deactivate functional peptides and proteins with high spatial and temporal resolution. Here we show that 2-nitrophenylalanine is able to photochemically cleave the polypeptide backbone by an unusual cinnoline-forming reaction. This unnatural amino acid was genetically encoded in E. coli, and protein containing 2-nitrophenylalanine was expressed and site-specifically photocleaved.

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Year:  2009        PMID： 19246005      PMCID： PMC2714363          DOI： 10.1016/j.chembiol.2009.01.013

Source DB:  PubMed          Journal:  Chem Biol        ISSN： 1074-5521


  14 in total

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