Literature DB >> 19134467

In vivo labeling method using a genetic construct for nanoscale resolution microscopy.

Jan Schröder1, Hélène Benink, Marcus Dyba, Georgyi V Los.   

Abstract

We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.

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Year:  2009        PMID： 19134467      PMCID： PMC2710050          DOI： 10.1016/j.bpj.2008.09.032

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  16 in total

1.  Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.

Authors:  T A Klar; S Jakobs; M Dyba; A Egner; S W Hell
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-18       Impact factor: 11.205

2.  STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.

Authors:  Katrin I Willig; Silvio O Rizzoli; Volker Westphal; Reinhard Jahn; Stefan W Hell
Journal:  Nature       Date:  2006-04-13       Impact factor: 49.962

3.  Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release.

Authors:  Robert J Kittel; Carolin Wichmann; Tobias M Rasse; Wernher Fouquet; Manuela Schmidt; Andreas Schmid; Dhananjay A Wagh; Christian Pawlu; Robert R Kellner; Katrin I Willig; Stefan W Hell; Erich Buchner; Manfred Heckmann; Stephan J Sigrist
Journal:  Science       Date:  2006-04-13       Impact factor: 47.728

Review 4.  Nanoscale resolution in GFP-based microscopy.

Authors:  Katrin I Willig; Robert R Kellner; Rebecca Medda; Birka Hein; Stefan Jakobs; Stefan W Hell
Journal:  Nat Methods       Date:  2006-09       Impact factor: 28.547

5.  Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy.

Authors:  R R Kellner; C J Baier; K I Willig; S W Hell; F J Barrantes
Journal:  Neuroscience       Date:  2006-10-16       Impact factor: 3.590

Review 6.  Interfering with leukocyte integrin activation--a novel concept in the development of anti-inflammatory drugs.

Authors:  Tiina J Hilden; Susanna M Nurmi; Susanna C Fagerholm; Carl G Gahmberg
Journal:  Ann Med       Date:  2006       Impact factor: 4.709

7.  Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.

Authors:  S W Hell; J Wichmann
Journal:  Opt Lett       Date:  1994-06-01       Impact factor: 3.776

8.  Alphavirus replicase protein NSP1 induces filopodia and rearrangement of actin filaments.

Authors:  P Laakkonen; P Auvinen; P Kujala; L Kääriäinen
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103

9.  The SNARE motif is essential for the formation of syntaxin clusters in the plasma membrane.

Authors:  Jochen J Sieber; Katrin I Willig; Rainer Heintzmann; Stefan W Hell; Thorsten Lang
Journal:  Biophys J       Date:  2006-01-27       Impact factor: 4.033

10.  Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.

Authors:  Soshana Svendsen; Chad Zimprich; Mark G McDougall; Dieter H Klaubert; Georgyi V Los
Journal:  BMC Cell Biol       Date:  2008-04-02       Impact factor: 4.241
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  16 in total

1.  STED nanoscopy in living cells using Fluorogen Activating Proteins.

Authors:  James A J Fitzpatrick; Qi Yan; Jochen J Sieber; Marcus Dyba; Ulf Schwarz; Chris Szent-Gyorgyi; Carol A Woolford; Peter B Berget; Alan S Waggoner; Marcel P Bruchez
Journal:  Bioconjug Chem       Date:  2009       Impact factor: 4.774

2.  HaloTag: a novel reporter gene for positron emission tomography.

Authors:  Hao Hong; Hélène A Benink; Yin Zhang; Yunan Yang; H Tetsuo Uyeda; Jonathan W Engle; Gregory W Severin; Mark G McDougall; Todd E Barnhart; Dieter H Klaubert; Robert J Nickles; Frank Fan; Weibo Cai
Journal:  Am J Transl Res       Date:  2011-07-28       Impact factor: 4.060

Review 3.  Fluorescence nanoscopy. Methods and applications.

Authors:  Jose Requejo-Isidro
Journal:  J Chem Biol       Date:  2013-06-04

Review 4.  Advances in chemical labeling of proteins in living cells.

Authors:  Qi Yan; Marcel P Bruchez
Journal:  Cell Tissue Res       Date:  2015-03-07       Impact factor: 5.249

Review 5.  Dynamic imaging of mitochondrial membrane proteins in specific sub-organelle membrane locations.

Authors:  Timo Appelhans; Karin B Busch
Journal:  Biophys Rev       Date:  2017-08-17

6.  Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.

Authors:  Davide Mazza; Timothy J Stasevich; Tatiana S Karpova; James G McNally
Journal:  Methods Mol Biol       Date:  2012

7.  STAQ: A route toward low power, multicolor nanoscopy.

Authors:  Tilman Rosales; Dan L Sackett; Jianhua Xu; Zhen-Dan Shi; Biying Xu; Haitao Li; Gurpreet Kaur; Erin Frohart; Nalini Shenoy; Sarah M Cheal; Haitao Wu; Andrés E Dulcey; Yulin Hu; Changhui Li; Kelly Lane; Gary L Griffiths; Jay R Knutson
Journal:  Microsc Res Tech       Date:  2015-03-12       Impact factor: 2.769

Review 8.  A guide to super-resolution fluorescence microscopy.

Authors:  Lothar Schermelleh; Rainer Heintzmann; Heinrich Leonhardt
Journal:  J Cell Biol       Date:  2010-07-19       Impact factor: 10.539

9.  HaloTag as a reporter gene: positron emission tomography imaging with (64)Cu-labeled second generation HaloTag ligands.

Authors:  Hao Hong; Hélène A Benink; H Tetsuo Uyeda; Hector F Valdovinos; Yin Zhang; Poncho Meisenheimer; Todd E Barnhart; Frank Fan; Weibo Cai
Journal:  Am J Transl Res       Date:  2013-04-19       Impact factor: 4.060

Review 10.  Single-Molecule Imaging in Living Plant Cells: A Methodological Review.

Authors:  Ai-Yu Guo; Ya-Mei Zhang; Liu Wang; Di Bai; Ya-Peng Xu; Wen-Qiang Wu
Journal:  Int J Mol Sci       Date:  2021-05-11       Impact factor: 5.923
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