Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Fluorescence nanoscopy. Methods and applications.

Literature DB >> 24432127

Fluorescence nanoscopy. Methods and applications.

Abstract

Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.

Keywords: Fluorescence nanoscopy; PALM; SIM; STED; STORM; Subdiffraction imaging; Super-resolution

Year: 2013 PMID： 24432127 PMCID： PMC3691399 DOI： 10.1007/s12154-013-0096-3

Source DB: PubMed Journal: J Chem Biol ISSN： 1864-6158

205 in total

1. Cell type-specific β2-adrenergic receptor clusters identified using photoactivated localization microscopy are not lipid raft related, but depend on actin cytoskeleton integrity.

Authors: Marco Scarselli; Paolo Annibale; Aleksandra Radenovic
Journal: J Biol Chem Date: 2012-03-22 Impact factor: 5.157

2. High-density mapping of single-molecule trajectories with photoactivated localization microscopy.

Authors: Suliana Manley; Jennifer M Gillette; George H Patterson; Hari Shroff; Harald F Hess; Eric Betzig; Jennifer Lippincott-Schwartz
Journal: Nat Methods Date: 2008-01-13 Impact factor: 28.547

3. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.

Authors: Gleb Shtengel; James A Galbraith; Catherine G Galbraith; Jennifer Lippincott-Schwartz; Jennifer M Gillette; Suliana Manley; Rachid Sougrat; Clare M Waterman; Pakorn Kanchanawong; Michael W Davidson; Richard D Fetter; Harald F Hess
Journal: Proc Natl Acad Sci U S A Date: 2009-02-06 Impact factor: 11.205

Fluorescence nanoscopy. Methods and applications.

1. Cell type-specific β2-adrenergic receptor clusters identified using photoactivated localization microscopy are not lipid raft related, but depend on actin cytoskeleton integrity.

2. High-density mapping of single-molecule trajectories with photoactivated localization microscopy.

3. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.

4. Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy.

5. STED nanoscopy of actin dynamics in synapses deep inside living brain slices.

6. Quantitative analysis of photoactivated localization microscopy (PALM) datasets using pair-correlation analysis.

7. The SNARE motif is essential for the formation of syntaxin clusters in the plasma membrane.

8. Maturation-dependent HIV-1 surface protein redistribution revealed by fluorescence nanoscopy.

9. Simultaneous multiple-emitter fitting for single molecule super-resolution imaging.

10. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.

1. Correlated confocal and super-resolution imaging by VividSTORM.

Review 2. Inside single cells: quantitative analysis with advanced optics and nanomaterials.

3. Superresolution live imaging of plant cells using structured illumination microscopy.

4. Single-cell in situ imaging of palmitoylation in fatty-acylated proteins.

Review 5. Recent Progress of Rare-Earth Doped Upconversion Nanoparticles: Synthesis, Optimization, and Applications.

6. The use of nanoscale fluorescence microscopic to decipher cell wall modifications during fungal penetration.

Review 7. Functional imaging for regenerative medicine.

8. KRAS regulation by small non-coding RNAs and SNARE proteins.