Literature DB >> 16896340

Nanoscale resolution in GFP-based microscopy.

Katrin I Willig1, Robert R Kellner, Rebecca Medda, Birka Hein, Stefan Jakobs, Stefan W Hell.   

Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

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Year:  2006        PMID： 16896340     DOI： 10.1038/nmeth922

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  93 in total

1.  Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution.

Authors:  E Hesper Rego; Lin Shao; John J Macklin; Lukman Winoto; Göran A Johansson; Nicholas Kamps-Hughes; Michael W Davidson; Mats G L Gustafsson
Journal:  Proc Natl Acad Sci U S A       Date:  2011-12-12       Impact factor: 11.205

2.  Two-color STED microscopy of living synapses using a single laser-beam pair.

Authors:  Jan Tønnesen; Fabien Nadrigny; Katrin I Willig; Roland Wedlich-Söldner; U Valentin Nägerl
Journal:  Biophys J       Date:  2011-11-15       Impact factor: 4.033

3.  Single-image diffusion coefficient measurements of proteins in free solution.

Authors:  Shannon Kian Zareh; Michael C DeSantis; Jonathan M Kessler; Je-Luen Li; Y M Wang
Journal:  Biophys J       Date:  2012-04-03       Impact factor: 4.033

4.  3D visualization of HIV transfer at the virological synapse between dendritic cells and T cells.

Authors:  Richard L Felts; Kedar Narayan; Jacob D Estes; Dan Shi; Charles M Trubey; Jing Fu; Lisa M Hartnell; Gordon T Ruthel; Douglas K Schneider; Kunio Nagashima; Julian W Bess; Sina Bavari; Bradley C Lowekamp; Donald Bliss; Jeffrey D Lifson; Sriram Subramaniam
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-12       Impact factor: 11.205

Review 5.  Imaging plasmodesmata.

Authors:  Karen Bell; Karl Oparka
Journal:  Protoplasma       Date:  2010-11-12       Impact factor: 3.356

6.  Visualization of the immunological synapse by dual color time-gated stimulated emission depletion (STED) nanoscopy.

Authors:  Emily M Mace; Jordan S Orange
Journal:  J Vis Exp       Date:  2014-03-24       Impact factor: 1.355

7.  Single-molecule mountains yield nanoscale cell images.

Authors:  W E Moerner
Journal:  Nat Methods       Date:  2006-10       Impact factor: 28.547

Review 8.  Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.

Authors:  Sohum Mehta; Jin Zhang
Journal:  Annu Rev Biochem       Date:  2011       Impact factor: 23.643

9.  New directions in single-molecule imaging and analysis.

Authors:  W E Moerner
Journal:  Proc Natl Acad Sci U S A       Date:  2007-07-30       Impact factor: 11.205

10.  Features of endogenous cardiomyocyte chromatin revealed by super-resolution STED microscopy.

Authors:  Scherise Mitchell-Jordan; Haodong Chen; Sarah Franklin; Enrico Stefani; Laurent A Bentolila; Thomas M Vondriska
Journal:  J Mol Cell Cardiol       Date:  2012-07-28       Impact factor: 5.000
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