Literature DB >> 20976031

STED nanoscopy in living cells using Fluorogen Activating Proteins.

James A J Fitzpatrick1, Qi Yan, Jochen J Sieber, Marcus Dyba, Ulf Schwarz, Chris Szent-Gyorgyi, Carol A Woolford, Peter B Berget, Alan S Waggoner, Marcel P Bruchez.   

Abstract

We demonstrate the effectiveness of a genetically encoded Malachite Green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with FWHM of 110-122nm were observed. Depletion data however suggests a resolution of 70nm with the given instrument.

Entities:  

Year:  2009        PMID： 20976031      PMCID： PMC2957894          DOI： 10.1021/bc900249e

Source DB:  PubMed          Journal:  Bioconjug Chem        ISSN： 1043-1802            Impact factor:   4.774


  21 in total

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8.  Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.

Authors:  S W Hell; J Wichmann
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  22 in total

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6.  Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule.

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Review 7.  Advances in chemical labeling of proteins in living cells.

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