Literature DB >> 1830475

Expression of a human monoclonal anti-(rhesus D) Fab fragment in Escherichia coli with the use of bacteriophage lambda vectors.

R A Williamson1, M A Persson, D R Burton.   

Abstract

A human anti-(rhesus D) antibody (IgG1 lambda) Fab fragment was cloned from an Epstein-Barr-virus-transformed cell line and expressed in Escherichia coli with the use of bacteriophage lambda vectors. The cloned protein is active in binding to human erythrocytes and permits the development of a recombinant reagent for the prevention of haemolytic disease of the newborn. The method offers a rapid and effective means of rescuing human Fabs from potentially unstable cell lines secreting human antibodies.

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Year:  1991        PMID： 1830475      PMCID： PMC1151271          DOI： 10.1042/bj2770561

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  Expression of engineered antibodies and antibody fragments in microorganisms.

Authors:  M Better; A H Horwitz
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

2.  In vitro development of human monoclonal antibody-secreting plasmacytomas.

Authors:  A Doyle; T J Jones; J L Bidwell; B A Bradley
Journal:  Hum Immunol       Date:  1985-07       Impact factor: 2.850

3.  Rh(D) haemolytic disease of the newborn: the changing scene.

Authors:  S J Urbaniak
Journal:  Br Med J (Clin Res Ed)       Date:  1985-07-06

Review 4.  Probing the human B-cell repertoire with EBV: polyreactive antibodies and CD5+ B lymphocytes.

Authors:  P Casali; A L Notkins
Journal:  Annu Rev Immunol       Date:  1989       Impact factor: 28.527

5.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

6.  Senescence of a human lymphoblastoid clone producing anti-Rhesus(D).

Authors:  M D Melamed; J Gordon; S J Ley; D Edgar; N C Hughes-Jones
Journal:  Eur J Immunol       Date:  1985-07       Impact factor: 5.532

7.  Production of human monoclonal antibody to rhesus D antigen.

Authors:  D H Crawford; M J Barlow; J F Harrison; L Winger; E R Huehns
Journal:  Lancet       Date:  1983-02-19       Impact factor: 79.321

8.  Human monoclonal antibodies to Rh0(D).

Authors:  S K Foung; J A Blunt; P S Wu; P Ahearn; L C Winn; E G Engleman; F C Grumet
Journal:  Vox Sang       Date:  1987       Impact factor: 2.144

9.  Human monoclonal antibodies against blood group antigens. Preparation of a series of stable EBV immortalized B clones producing high levels of antibody of different isotypes and specificities.

Authors:  D Goossens; F Champomier; P Rouger; C Salmon
Journal:  J Immunol Methods       Date:  1987-08-03       Impact factor: 2.303

10.  Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.

Authors:  W D Huse; L Sastry; S A Iverson; A S Kang; M Alting-Mees; D R Burton; S J Benkovic; R A Lerner
Journal:  Science       Date:  1989-12-08       Impact factor: 47.728
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  5 in total

1.  Obtaining a functional recombinant anti-rhesus (D) antibody using the baculovirus-insect cell expression system.

Authors:  L Edelman; C Margaritte; H Chaabihi; E Monchâtre; D Blanchard; A Cardona; F Morin; G Dumas; S Petres; M Kaczorek
Journal:  Immunology       Date:  1997-05       Impact factor: 7.397

2.  A recombinant human Fab expressed in Escherichia coli neutralizes rabies virus.

Authors:  S C Cheung; B Dietzschold; H Koprowski; A L Notkins; R F Rando
Journal:  J Virol       Date:  1992-11       Impact factor: 5.103

3.  Human combinatorial antibody libraries to hepatitis B surface antigen.

Authors:  S L Zebedee; C F Barbas; Y L Hom; R H Caothien; R Graff; J DeGraw; J Pyati; R LaPolla; D R Burton; R A Lerner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

4.  Inhibition of hepatitis C virus nonstructural protein, helicase activity, and viral replication by a recombinant human antibody clone.

Authors:  Ramesh Prabhu; Nutan Khalap; Roberto Burioni; Massimo Clementi; Robert F Garry; Srikanta Dash
Journal:  Am J Pathol       Date:  2004-10       Impact factor: 4.307

5.  Incomplete Antibodies May Reduce ABO Cross-Match Incompatibility: A Pilot Study.

Authors:  Mehmet Özen; Soner Yılmaz; Tülin Özkan; Yeşim Özer; Aliye Aysel Pekel; Asuman Sunguroğlu; Günhan Gürman; Önder Arslan
Journal:  Turk J Haematol       Date:  2017-05-23       Impact factor: 1.831
  5 in total

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