Literature DB >> 2989228

In vitro development of human monoclonal antibody-secreting plasmacytomas.

A Doyle, T J Jones, J L Bidwell, B A Bradley.   

Abstract

A polyclonal human lymphoblastoid cell line transformed in vitro with Epstein-Barr virus produced specific anti-Rhesus D antibody. It was repeatedly enriched by rosetting procedures and subsequently cloned. The cloning conditions employed a combination of mouse macrophage feeder layers, antimycoplasma agents, and low density passage. Formal evidence of monoclonality was obtained in one case which was of human IgG1 isotype and was secreted at the level of 15-20 micrograms/ml. All clones showed long-term stability in culture after 10 months of continuous passage. Both polyclonal and monoclonal cell lines possessed antigens characteristic of highly differentiated B cells, yet they also expressed Epstein-Barr virus nuclear antigen (EBNA). This study exemplifies a simple method for obtaining monoclonal antibody secreting plasmacytomas of human origin.

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Year:  1985        PMID： 2989228     DOI： 10.1016/0198-8859(85)90012-6

Source DB:  PubMed          Journal:  Hum Immunol        ISSN： 0198-8859            Impact factor:   2.850


  7 in total

1.  Distinctive role of IgG1 and IgG3 isotypes in Fc gamma R-mediated functions.

Authors:  Z Rozsnyay; G Sármay; M Walker; K Maslanka; Z Valasek; R Jefferis; J Gergely
Journal:  Immunology       Date:  1989-04       Impact factor: 7.397

2.  Expression of a human monoclonal anti-(rhesus D) Fab fragment in Escherichia coli with the use of bacteriophage lambda vectors.

Authors:  R A Williamson; M A Persson; D R Burton
Journal:  Biochem J       Date:  1991-07-15       Impact factor: 3.857

3.  Identification and partial characterization of the human erythrocyte membrane component(s) that express the antigens of the LW blood-group system.

Authors:  G Mallinson; P G Martin; D J Anstee; M J Tanner; A H Merry; D Tills; H H Sonneborn
Journal:  Biochem J       Date:  1986-03-15       Impact factor: 3.857

4.  Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific.

Authors:  N Avent; P A Judson; S F Parsons; G Mallinson; D J Anstee; M J Tanner; P R Evans; E Hodges; A G Maciver; C Holmes
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

5.  Genetic variants of human red-cell membrane sialoglycoprotein beta. Study of the alterations occurring in the sialoglycoprotein-beta gene.

Authors:  M J Tanner; S High; P G Martin; D J Anstee; P A Judson; T J Jones
Journal:  Biochem J       Date:  1988-03-01       Impact factor: 3.857

6.  The Ina and Inb blood group antigens are located on a glycoprotein of 80,000 MW (the CDw44 glycoprotein) whose expression is influenced by the In(Lu) gene.

Authors:  F A Spring; R Dalchau; G L Daniels; G Mallinson; P A Judson; S F Parsons; J W Fabre; D J Anstee
Journal:  Immunology       Date:  1988-05       Impact factor: 7.397

7.  Reaction of rheumatoid factors with IgG3 monoclonal anti-Rh(D) antibodies: more frequent reactivity to a monoclonal antibody of the Gm allotype G3m(5) in rheumatoid patients negative for G3m(5).

Authors:  A H Puttick; E A Williamson; A H Merry; B M Kumpel; K M Thompson; V E Jones
Journal:  Ann Rheum Dis       Date:  1988-11       Impact factor: 19.103
  7 in total

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