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Literature DB >> 2531466

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.

W D Huse¹, L Sastry, S A Iverson, A S Kang, M Alting-Mees, D R Burton, S J Benkovic, R A Lerner.

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

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Year: 1989 PMID： 2531466 DOI： 10.1126/science.2531466

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

144 in total

Review 1. Generation of recombinant antibodies.

Authors: S M Kipriyanov; M Little
Journal: Mol Biotechnol Date: 1999-09 Impact factor: 2.695

2. N-terminal mutations in the anti-estradiol Fab 57-2 modify its hapten binding properties.

Authors: P Saviranta PJauria; U Lamminmäki; J Hellman; S Eriksson; T Lövgren
Journal: Protein Sci Date: 2000-12 Impact factor: 6.725

3. Molecular basis of lutropin recognition by the mannose/GalNAc-4-SO4 receptor.

Authors: D S Roseman; J U Baenziger
Journal: Proc Natl Acad Sci U S A Date: 2000-08-29 Impact factor: 11.205

4. A binary plasmid system for shuffling combinatorial antibody libraries.

Authors: T A Collet; P Roben; R O'Kennedy; C F Barbas; D R Burton; R A Lerner
Journal: Proc Natl Acad Sci U S A Date: 1992-11-01 Impact factor: 11.205

Review 5. The polymerase chain reaction and other amplification techniques in immunological research and diagnosis.

Authors: A M Lew; R B Brandon; M Panaccio; C J Morrow
Journal: Immunology Date: 1992-01 Impact factor: 7.397

6. In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells.

Authors: M J Embleton; G Gorochov; P T Jones; G Winter
Journal: Nucleic Acids Res Date: 1992-08-11 Impact factor: 16.971

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.

Review 1. Generation of recombinant antibodies.

2. N-terminal mutations in the anti-estradiol Fab 57-2 modify its hapten binding properties.

3. Molecular basis of lutropin recognition by the mannose/GalNAc-4-SO4 receptor.

4. A binary plasmid system for shuffling combinatorial antibody libraries.

Review 5. The polymerase chain reaction and other amplification techniques in immunological research and diagnosis.

6. In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells.

Review 7. Adapting antibodies for clinical use.

Review 8. Molecular engineering of antibodies for therapeutic and diagnostic purposes.

9. Therapeutic antibodies, vaccines and antibodyomes.

10. Production and Characterization of Neutralizing Antibodies against Bungarus multicinctus Snake Venom.