Literature DB >> 2440339

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

P Chomczynski, N Sacchi.   

Abstract

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

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Year:  1987        PMID： 2440339     DOI： 10.1006/abio.1987.9999

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  2000 in total

1.  Volvoxrhodopsin, a light-regulated sensory photoreceptor of the spheroidal green alga Volvox carteri.

Authors:  E Ebnet; M Fischer; W Deininger; P Hegemann
Journal:  Plant Cell       Date:  1999-08       Impact factor: 11.277

2.  Rheumatoid factors in primary Sjögren's syndrome (pSS) use diverse VH region genes, the majority of which show no evidence of somatic hypermutation.

Authors:  K E Elagib; M Børretzen; R Jonsson; H J Haga; J Thoen; K M Thompson; J B Natvig
Journal:  Clin Exp Immunol       Date:  1999-08       Impact factor: 4.330

3.  Expression of GDNF and GDNFR-alpha mRNAs in muscles of patients with motor neuron diseases.

Authors:  M Yamamoto; N Mitsuma; A Inukai; Y Ito; M Li; T Mitsuma; G Sobue
Journal:  Neurochem Res       Date:  1999-06       Impact factor: 3.996

4.  The expression of several mitochondrial and nuclear genes encoding the subunits of electron transport chain enzyme complexes, cytochrome c oxidase, and NADH dehydrogenase, in different brain regions in Alzheimer's disease.

Authors:  M Y Aksenov; H M Tucker; P Nair; M V Aksenova; D A Butterfield; S Estus; W R Markesbery
Journal:  Neurochem Res       Date:  1999-06       Impact factor: 3.996

5.  The GDVII strain of Theiler's virus spreads via axonal transport.

Authors:  C Martinat; N Jarousse; M C Prévost; M Brahic
Journal:  J Virol       Date:  1999-07       Impact factor: 5.103

6.  Cloning and characterization of the dihydrolipoamide S-acetyltransferase subunit of the plastid pyruvate dehydrogenase complex (E2) from Arabidopsis.

Authors:  B P Mooney; J A Miernyk; D D Randall
Journal:  Plant Physiol       Date:  1999-06       Impact factor: 8.340

7.  A strong negative transcriptional regulatory region between the human cytomegalovirus UL127 gene and the major immediate-early enhancer.

Authors:  C A Lundquist; J L Meier; M F Stinski
Journal:  J Virol       Date:  1999-11       Impact factor: 5.103

8.  Developmental genetics of the essential Drosophila nucleoporin nup154: allelic differences due to an outward-directed promoter in the P-element 3' end.

Authors:  A A Kiger; S Gigliotti; M T Fuller
Journal:  Genetics       Date:  1999-10       Impact factor: 4.562

9.  Diversity and distribution of nicotinic acetylcholine receptors in the locus ceruleus neurons.

Authors:  C Léna; A de Kerchove D'Exaerde; M Cordero-Erausquin; N Le Novère; M del Mar Arroyo-Jimenez; J P Changeux
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

10.  Integrin-mediated adhesion of endothelial cells induces JAK2 and STAT5A activation: role in the control of c-fos gene expression.

Authors:  M F Brizzi; P Defilippi; A Rosso; M Venturino; G Garbarino; A Miyajima; L Silengo; G Tarone; L Pegoraro
Journal:  Mol Biol Cell       Date:  1999-10       Impact factor: 4.138
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